The FTIR spectra were collected in the 4000–650 cm−1 range, with a resolution of 5 cm−1 at room temperature by using a Jasco FT/IR-4600 spectrometer provided with single ATR accessory (Jasco ATR PRO ONE). The number of accumulated scans for each recorded spectrum was automatic whit an average exposure around 1.5 min. All the experiments were performed at room temperature.
Atr pro one
Manufactured by Jasco
Sourced in Japan, United States
The ATR PRO ONE is a compact, portable Attenuated Total Reflectance (ATR) accessory designed for use with Fourier Transform Infrared (FTIR) spectrometers. It provides a simple and efficient way to analyze solid and liquid samples without the need for extensive sample preparation.
Automatically generated - may contain errors
Lab products found in correlation
Ft ir 4700, by Jasco (5 mentions)
Labspec, by Horiba (3 mentions)
Ft ir 4600 spectrometer, by Jasco (2 mentions)
Ft ir 4200, by Jasco (2 mentions)
Ft ir 4600, by Jasco (2 mentions)
Origin 8, by OriginLab (2 mentions)
Originpro 8, by OriginLab (2 mentions)
Jem 1400, by JEOL (1 mentions)
D2 phaser, by Bruker (1 mentions)
Spectra manager, by Jasco (1 mentions)
Spectra analysis, by Jasco (1 mentions)
Magnetic stirrer, by Heidolph (1 mentions)
Ethanol bioultra, by Merck Group (1 mentions)
Atr ft ir 4600, by Jasco (1 mentions)
Viscosimeter brookfield dv2t, by Ametek (1 mentions)
Densimeter da 100m, by Mettler Toledo (1 mentions)
Discover sp d oven, by CEM Corporation (1 mentions)
V 730, by Jasco (1 mentions)
Uv vis 1700 spectrophotometer, by Shimadzu (1 mentions)
Diamond prism, by Jasco (1 mentions)
23 protocols using atr pro one
1
FTIR Spectroscopy of Samples
2
FTIR Analysis of Powder Samples
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FTIR measurements were carried out using an FTIR spectrometer (Jasco, 6800 FV, Tokyo, Japan) equipped with a Diamond ATR device (Jasco, ATR Pro One, Tokyo, Japan). The effective dimensions of the diamond are 1.8 mm diameter. The refractive index of the diamond is 2.4 and the angle of incidence in our device was 45 degree, generating 1 reflection. The calculated depth of penetration is ~2 μm. The radiation from the IR source of the spectrometer was focused into the ATR diamond and the output radiation (from the other side of the diamond) was focused onto a DLaTGS (Deuterated Lanthanum α Alanine doped TriGlycine Sulphate) detector. Powder samples were placed on the diamond ATR and pressure (700 kg/cm2) was applied to produce better contact between the sample and the diamond. To trace the PDL in the samples, we compared the samples’ spectra with the spectrum of PDL powder. Measurements were carried out in the spectral range of 650–4000 cm−1. Each spectrum was an average of 64 scans to increase the signal to noise ratio (SNR). The spectra were analyzed using Spectra AnalysisTM (Jasco, Tokyo, Japan).
3
Characterization of Oleylamine-Capped Silver Nanoparticles
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The surface ligands of the OAm-Ag NP powders were determined by Fourier transform infrared (FT-IR) spectroscopy (FT/IR-4200, JASCO Corporation, Tokyo, Japan; measurement range: 500–4000 cm−1, resolution: 4 cm−1, number of scans: 128) using an attenuated total reflection (ATR) instrument (ATR PRO ONE, JASCO Corporation, Tokyo, Japan). The crystal structures of the OAm-Ag NP powders were determined by X-ray diffraction (XRD) analysis (D2 Phaser, Bruker AXS GmbH, Karlsruhe, Germany, 2θ range: 10–80°, Cu-Kα radiation source (λ = 1.5406 Å) at 30 kV accelerating voltage and 10 mA current). Transmission electron microscopy (TEM) of the OAm-Ag NPs was conducted using a microscope (JEOL JEM1400, Tokyo, Japan) operated at 120 kV.
4
FTIR Analysis of PSI Fibers and Composites
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For the FTIR studies, a JASCO 4700A device equipped with an ATR diamond crystal (JASCO Ltd., Budapest, Hungary, ATR Pro ONE) and with a DTGS detector was used. For taking the spectra, 128 parallel measurements were made in the 4000–400 cm−1 range, with a 4 cm−1 resolution in all cases. Final spectra were obtained after water, CO2 and baseline corrections. In addition, the FTIR spectra of non-treated PSI fibres, iron oxide and PSI-DAB-Magn samples were taken.
5
Infrared Spectroscopic Analysis of Galled Leaves
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Randomly collected fresh control (ungalled) and galled leaf samples were subjected to ATR analysis following the method of Luz [15 (link)] with slight modifications. Being the preferred site for mite infestation, the abaxial/lower leaf surface was subjected to IR measurement. The leaves were placed in direct contact with ZnSe/Diamond crystal of FTIR 4600 equipped with accessory Jasco ATR Pro One device. All samples were placed in the same position to ensure optimum contact with crystal, using standard pressing mechanism of the instrument, maintaining constant pressure for all samples. Spectra reading was made thrice for each sample to avoid noise absorption peaks and average spectral details were used for later analysis. The spectra were collected over 4000 to 400 cm− 1 range (wave numbers), with resolution 4 cm− 1 and total of 25 scans per each sample with three replicas.
6
FTIR Spectroscopy Analysis of Samples
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Fourier Transformed Infra-Red spectroscopy (FTIR) spectra were collected at room temperature using an FTIR spectrometer (ATR-FTIR, FTIR-4700 with ATR PRO ONE equipped with a diamond prism, Jasco Co., Tokyo, Japan) with a Michelson 28-degree interferometer with corner-cube mirrors with a range between 250,000 and 5 cm−1. The aperture size was 200 × 200 µm2 the acquisition time was fixed to 30 s. The instrument was operated using (Spectra Manager, JASCO, Tokyo, Japan) software. A total of three samples of each type were scanned from 400 and 4000 cm−1 at 5 different locations. The spectra were analyzed with (OriginLab Co., Northampton, MA, USA, and LabSpec, Horiba/Jobin-Yvon, Kyoto, Japan) software.
7
Decalcification and FTIR Analysis of Shell
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One shell fragment (∼1 cm2) was immersed in a decalcifying and etching solution (0.05M EDTA + 0.25 M HEPES + 2.5% Glutaraldehyde) until complete decalcification. The organic residue was washed with ultrapure distilled water (milli Q) and oven-dried. It was analyzed with an attenuated total reflection (ATR) diamond unit (ATR Pro One, Jasco) (Department of Mineralogy, UGR). The IR spectra were recorded at a 2 cm-1 resolution over 100 scans using a Fourier transform infrared (FTIR) spectrometer Jasco 6600. Four measurements were carried out in different areas of the membrane to obtain a coherent assertion (standard deviation and error) of the results.
8
FTIR Analysis of Cell Suspensions
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Measurements were carried out using an FTIR spectrometer (Jasco, 6800 FV, Tokyo, Japan) equipped with a Diamond ATR device (Jasco, ATR Pro One, Tokyo, Japan). The effective dimensions of the diamond are 1.8 mm diameter. The refractive index of the diamond is 2.4 and the angle of incidence in our device was 45°, generating 1 reflection. The calculated depth of penetration is ~ 2 μm. The radiation from the IR source of the spectrometer was focused into the ATR diamond, and the output radiation (from the other side of the diamond) was focused onto a DLaTGS (Deuterated Lanthanum α Alanine doped TriGlycine Sulphate) detector. 10–20 µl of cell suspensions of specific lineages were placed on the diamond ATR. Pressure (700 kg/cm2) was applied to produce better contact between the sample and the diamond. Measurements were carried out in the spectral range of 4000–650 cm−1. Each spectrum at acquisition (see Supplementary) was an average of 64 scans to increase the signal to noise ratio (SNR). The spectra were analyzed using Spectra Analysis™ (Jasco, Tokyo, Japan).
9
Aqueous Peptide-NaOH-UO2 FTIR Spectroscopy
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An aqueous sample solution (1 mL) dissolving peptide, NaOH, and UO 2 2+ in a selected molar ratio was dropped onto a diamond prism of an ATR attachment (JASCO ATR PRO ONE) of an FTIR spectrophotometer (JASCO FT/IR-4700). After drying the sample solution under airflow at room temperature, the IR spectrum of the residue on the prism was obtained 64 times and merged.
10
Chitosan Membrane Characterization by ATR-FTIR
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The attenuated total reflection–Fourier transform infrared (ATR-FTIR) spectrum analysis (JASCO FT/IR-4600 type A with ATR-PRO-ONE and TGS (ABL&E-JASCO Poland, Kraków, Poland)) was applied to characterise the structures of chitosan membranes. The spectra were collected over the range of 300–4000 cm−1 with resolution 4 cm−1 and at a scanning speed of 2 mm/s.
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