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Rabbit anti adam10

Manufactured by Abcam
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Rabbit anti-ADAM10 is a primary antibody that recognizes the ADAM10 protein. ADAM10 is a member of the ADAM (a disintegrin and metalloproteinase) family of proteins, which are involved in the proteolytic processing of cell surface proteins. The antibody can be used to detect and study the expression and localization of ADAM10 in various biological samples.

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Lab products found in correlation

Rabbit anti n cadherin, by Cell Signaling Technology (1 mentions) Hrp conjugated swine anti rabbit secondary antibody, by Agilent Technologies (1 mentions) Mouse anti 6e10, by BioLegend (1 mentions) Mouse anti tau5, by Thermo Fisher Scientific (1 mentions) Rabbit anti adam17, by Abcam (1 mentions) Enhanced chemiluminescence kit, by Bio-Rad (1 mentions) Anti rabbit and anti mouse hrp conjugated secondary antibodies, by Cell Signaling Technology (1 mentions) Mouse anti map2, by Merck Group (1 mentions) Mouse anti nestin, by Santa Cruz Biotechnology (1 mentions) Mouse anti vinculin, by Merck Group (1 mentions) Mouse anti gapdh, by Thermo Fisher Scientific (1 mentions) Iq imagequant tl software, by GE Healthcare (1 mentions) Las 4000 mini, by GE Healthcare (1 mentions) Rabbit anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Pageruler plus prestained protein ladder, by Thermo Fisher Scientific (1 mentions) Bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Abcam (1 mentions) Rabbit anti vamp2, by Cell Signaling Technology (1 mentions) Goat anti notch1, by Santa Cruz Biotechnology (1 mentions) Rabbit anti foxp2, by Abcam (1 mentions)

Close spelling variants of this product

Anti-ADAM10 ADAM10 Rabbit anti-

5 protocols using rabbit anti adam10

1

Protein Transfer and Immunoblotting

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After electrophoresis on 12% acrylamide gel, proteins were transferred on nitrocellulose PVDF membrane (Perkin Elmer Life Sciences, Waltham, MA, USA). Primary antibodies used were rabbit anti-ADAM10 (Abcam; 1/1000) or rabbit anti-N-cadherin (4061, Cell signalling; 1/500). Revelation was performed by chemiluminescence after incubation with HRP-conjugated swine anti-rabbit secondary antibody (Dako, 1/3000). Actin or GAPDH were detected as a loading control.
Sépult C., Bellefroid M., Rocks N., Donati K., Gérard C., Gilles C., Ludwig A., Duysinx B., Noël A, & Cataldo D. (2019). ADAM10 mediates malignant pleural mesothelioma invasiveness. Oncogene, 38(18), 3521-3534.
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2

Quantification of Alzheimer's Biomarkers

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We used the following primary antibodies for this study: mouse anti-4G8 (1:1,000, BioLegend, San Diego, CA, USA), mouse anti-6E10 (1:1,000, BioLegend, San Diego, CA, USA), rabbit anti-APP N-terminus (1:1,000, Sigma, Ronkonkoma, NY, USA), rabbit anti-ADAM8 (1:200, LSBio, Seattle, WA, USA), rabbit anti-ADAM9 (1:200, Abcam, UK), rabbit anti-ADAM10 (1:200, Abcam, UK), rabbit anti-ADAM12 (1:200, Abcam, UK), rabbit anti-ADAM17 (1:200, Abcam, UK), mouse anti-BACE1 (1:200, Abcam, UK), rabbit anti-neprilysin (1:200, Millipore, Burlington, MA, USA), mouse anti-AT100 (1:500, Invitrogen, Carlsbad, CA, USA), mouse anti-AT180 (1:500, Invitrogen, Carlsbad, CA, USA), and mouse anti-Tau5 (1:500, Invitrogen, Carlsbad, CA, USA).
Nam Y., Joo B., Lee J.Y., Han K.M., Ryu K.Y., Koh Y.H., Kim J., Koo J.W., We Y.M, & Hoe H.S. (2019). ALWPs Improve Cognitive Function and Regulate Aβ Plaque and Tau Hyperphosphorylation in a Mouse Model of Alzheimer’s Disease. Frontiers in Molecular Neuroscience, 12, 192.
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3

Western Blot Analysis of Protein Targets

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Standard methodologies were used. Protein extracts were separated by 10% or 8% SDS-PAGE and transferred to a PVDF membrane. Membranes were incubated using the following specific antibodies, including mouse anti-puromycin (1:500, DSHB), mouse anti-Vinculin (1:2000, Merck), mouse anti-GAPDH (1:2000, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-APP (1:2000, Merck), rabbit anti-ADAM10 (1:500, Abcam, Cambridge, UK), mouse anti-sAPPα (1:500, IBL America, Minneapolis, MN, USA), rabbit anti-OCT3/4 (1:1000, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-MAP2 (1:2000, Merck), mouse anti-Nestin (1:1000 Santa Cruz Biotechnology), mouse anti-SAP97 (1:1000, ENZO Life Sciences, Farmingdale, NY, USA) and rabbit anti-FMRP (1:1000, produced in house PZ1 [52 (link)]), HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (1:5000, Cell Signaling Technology, Danvers, MA, USA). Proteins were revealed using an enhanced chemiluminescence kit (Bio-Rad, Hercules, CA, USA) and the imaging system LAS-4000 mini (GE Healthcare, Chicago, IL, USA). Quantification was performed using the IQ ImageQuant TL software (GE Healthcare). Detection of GAPDH, Vinculin, and Coomassie staining were used as normalizers. For all SDS-PAGE PageRuler™ Plus Prestained Protein Ladder (10 to 250 kDa, Thermo Fisher Scientific) was used.
Cencelli G., Pacini L., De Luca A., Messia I., Gentile A., Kang Y., Nobile V., Tabolacci E., Jin P., Farace M.G, & Bagni C. (2023). Age-Dependent Dysregulation of APP in Neuronal and Skin Cells from Fragile X Individuals. Cells, 12(5), 758.
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4

Quantitative Protein Analysis of DRG Neurons

Cited 1 time
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Total proteins from cultured DRG neurons were isolated on DIV6 according to published protocols [25 (link), 27 (link)]. Total protein from DRG, sciatic nerve and foot pad tissues of db/db and db/m mice were extracted using the same methods. In vitro samples from 4 individual microfluidic chambers were pooled for one Western blot. The protein concentration was measured using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific). Western blot was performed according to previously described methods [25 (link), 27 (link)]. Briefly, equal amounts of proteins were loaded. Primary antibodies were rabbit anti-ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10, 1:1000, Abcam, Cambridge, UK), rabbit anti-DCX (doublecortin, 1:500, Abcam), rabbit anti-c-MET (tyrosine-protein kinase met, 1:500, Abcam), rabbit anti-FOXP2 (1:1000, Abcam), goat anti-NOTCH1 (1:1000, Santa Cruz), rabbit anti-ROCK1 (Rho associated coiled-coil containing protein kinase 1, 1:500, Abcam), rabbit anti-SYNJ1 (Synaptojanin 1, 1:1000, Sigma-Aldrich), rabbit anti-VAMP2 (vesicle-associated membrane protein 2, 1:1000, Cell Signaling Technology, Danvers, MA, USA), goat anti-VAT1 (1:1000, Santa Cruz), and mouse anti-β-actin (1:10000; Abcam). The optical density of protein bands was measured and calculated by means of Fluorchem E instrument (ProteinSimple, San Jose, CA, USA).
Jia L., Chopp M., Wang L., Lu X., Zhang Y., Szalad A, & Zhang Z.G. (2018). MiR-34a Regulates Axonal Growth of Dorsal Root Ganglia Neurons by Targeting FOXP2 and VAT1 in Postnatal and Adult Mouse. Molecular neurobiology, 55(12), 9089-9099.
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5

Antibody Panel for Protein Analysis

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The following antibodies were used: mouse anti-beta-actin (Santa Cruz, catalogue number sc-47778; WB 1:2000), rabbit anti-TACE/ADAM17 (Abcam, catalogue number ab39161; WB 1:2000), rabbit anti-HA (Santa-Cruz, catalogue number sc-805; IF 1:1000), mouse anti-HA (ENZO, catalogue number ENZ-ABS120-0200; PM IP 1:1000), mouse anti-transferrin receptor (Invitrogen, catalogue number 13–6800; 1:1000), rabbit anti-phosphoserine (Invitrogen, catalogue number 618100; WB 1:1000), rat HA-HRP, clone 3F10 (Roche, catalogue number 12013819001; WB 1:2000), mouse anti-p97 (Pierce/Thermo, catalogue number MA1-21412; WB 1:1000), rabbit anti-pan14-3-3 (Cell Signalling, catalogue number 8312; WB 1:1000), mouse anti-GM130 (BD Transduction labs, catalogue number 610823; IF 1:1000), rabbit anti-pan-cadherin (Cell Signalling, catalogue number 4068S; WB 1:1000), rabbit anti-ADAM10 (Abcam, catalogue number ab1997; WB 1:1000), rabbit anti-ERK1/2 (Cell Signalling, catalogue number 9102, WB 1:1000), rabbit anti-pERK1/2 (Cell Signalling, catalogue number 4377, WB 1:1000). Corresponding species-specific HRP or fluorescently coupled secondary antibodies were used from Santa Cruz and Cell Signaling (WB) or Invitrogen (IF).
Grieve A.G., Xu H., Künzel U., Bambrough P., Sieber B, & Freeman M. (2017). Phosphorylation of iRhom2 at the plasma membrane controls mammalian TACE-dependent inflammatory and growth factor signalling. eLife, 6, e23968.
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