Stem material from wild‐type and pC4H::schl::qsuB‐1 plants was extracted and ball‐milled for 3 h per 500 mg of sample (in 10 min on/10 min off cycles) using a PM100 ball mill (Retsch, Newtown, PA, USA) vibrating at 600 rpm in zirconium dioxide vessels (50 mL) containing ZrO2 ball bearings (10 × 10 mm). Ball‐milled walls were digested four times over 3 days at 50 °C with the polysaccharidases Cellic CTec2 and HTec2 (Novozymes, Davis, CA, USA) and pectinase from Aspergillus niger (Sigma‐Aldrich, St. Louis, MO, USA) in sodium citrate buffer (pH 5.0). The obtained cellulolytic lignin was washed with deionized water and lyophilized overnight.
Pectinase from aspergillus niger
Manufactured by Merck Group
Sourced in United States, Germany
Pectinase from Aspergillus niger is an enzyme that catalyzes the breakdown of pectin, a complex polysaccharide found in the cell walls of plants. It is derived from the filamentous fungus Aspergillus niger. Pectinase hydrolyzes the glycosidic bonds in pectin, which can be used in various industrial processes, such as fruit juice clarification, wine production, and textile processing.
Automatically generated - may contain errors
Lab products found in correlation
D galacturonic acid monohydrate, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Calcium chloride, by Avantor (2 mentions)
Sodium azide, by Merck Group (2 mentions)
Cellic ctec2, by Novozymes (1 mentions)
Htec2, by Novozymes (1 mentions)
D glucose, by Merck Group (1 mentions)
Sodium potassium tartrate, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Phenol, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Lach-Ner (1 mentions)
Xylose, by Merck Group (1 mentions)
Sodium carboxymethyl cellulose, by Merck Group (1 mentions)
Beechwood xylan, by Biosynth (1 mentions)
Cellulase from aspergillus niger, by Merck Group (1 mentions)
Pectin from citrus peel, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Cyanidin chloride, by Merck Group (1 mentions)
10 protocols using pectinase from aspergillus niger
1
Isolation and Characterization of Lignocellulosic Biomass
2
Comprehensive Chemical Reagents Inventory
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following chemicals were used: 3,5-dinitrosalicylic acid (DNSA) (98%, Thermo Fisher Scientific, Maharahstra, India), sodium sulphite (Lach-ner, Brno, Chez Republic), sodium hydroxide (Lach-ner, Brno, Chez Republic), phenol (99 + %, Thermo Fisher Scientific, Maharahstra, India), potassium sodium tartrate (Sigma-Aldrich, Buchs, Switzerland), Folin–Ciocalteu reagent (Sigma-Aldrich, Buchs, Switzerland), anhydrous sodium carbonate (Lach-ner, Brno, Chez Republic), gallic acid (anhydrous) for synthesis (Merck, Darmstadt, Germany), cellulase (from Aspergillus niger) (Sigma-Aldrich, Tokyo, Japan), pectinase (from Aspergillus niger) (Sigma-Aldrich, Buchs, Switzerland), xylanase (from Theryomyces, expressed in Aspergillus oryzae) (Sigma-Aldrich, Søborg, Denmark), beechwood xylan (Biosynth, Berkshir, England, UK), pectin from citrus peel (74.0%, Sigma-Aldrich, Buchs, Switzerland), sodium carboxymethyl cellulose (Sigma-Aldrich, Buchs, Switzerland), xylose (99%, Sigma-Aldrich, Buchs, Switzerland, D-( + )-glucose (99.5%, Sigma-Aldrich, Buchs, Switzerland), D-( + )-galacturonic acid monohydrate (97.0%, Sigma Aldrich, Buchs, Switzerland), C9-C25 alkanes, deuterated chloroform for NMR spectroscopy (CDCl3-d with 0.03% v/v TMS, 99.80%, Eurisotop, Saint-Aubin, France).
3
Peach Juice Polyphenol Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Refined KA120R k-carrageenan was purchased from Greenfresh Food Co., Ltd. (Longhai City, Zhangzhou, Fujian, China). The (-SO3-) content in k-carrageenan was 11.7 ± 0.7%. Peach juice concentrate was purchased from a local supermarket. The reagents, including the Folin and Ciocalteu’s phenol reagent, pectinase from Aspergillus niger (>1 U/mg), D-(+)-galacturonic acid monohydrate (99.1%), and ferulic acid (99.8%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). To build calibration curves, we used: 2,4-dichlorophenoxyacetic acid (GSO 9105-2008, Ecolan, Russia), D-(+)-glucose monohydrate (99.9%; Panreac Química SLU, Barcelona, Spain); and 3,5-dimethylphenol (99.8%, Acros Organics, Waltham, MA, USA).
4
Optimization of Anthocyanin Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The solvent used for the optimization of the extraction procedures was either methanol or ethanol of HPLC purity acquired from Fisher Scientific (Loughborough, UK) and Milli-Q water obtained by means of a Milli–Q water purification system (Millipore, Bedford, MA, USA). The liquid–liquid mixture at different percentages of the extraction solvent were prepared and then the pH was adjusted using HCl 1 M and NaOH 0.5 M solutions (Panreac, Barcelona, Spain).
For the analysis and quantification of the anthocyanins in the extracts, the following solvents were employed: methanol of HPLC purity (Fisher Scientific, Loughborough, UK) and formic acid of HPLC grade (Panreac, Barcelona, Spain). In addition, cyanidin chloride (95% purity, Sigma–Aldrich Chemical Co., St. Louis, MO, USA), was employed as the reference standard for the quantification of the anthocyanins. The enzyme used for the present study was a pectinase from Aspergillus niger (P4716–25KU, Sigma–Aldrich Chemical Co., St. Louis, MO, USA).
For the analysis and quantification of the anthocyanins in the extracts, the following solvents were employed: methanol of HPLC purity (Fisher Scientific, Loughborough, UK) and formic acid of HPLC grade (Panreac, Barcelona, Spain). In addition, cyanidin chloride (95% purity, Sigma–Aldrich Chemical Co., St. Louis, MO, USA), was employed as the reference standard for the quantification of the anthocyanins. The enzyme used for the present study was a pectinase from Aspergillus niger (P4716–25KU, Sigma–Aldrich Chemical Co., St. Louis, MO, USA).
5
Extraction and Characterization of Agar-Derived Polysaccharides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Agar (A) powder was purchased from Zhenpai Hydrocolloids Co., Ltd. (Zhangzhou City, Fujian Province, China), with moisture 7.97 wt%, pH 6.46, and 1.5% gel strength of 1150 g/cm2. FP was isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 0.8 [20 (link)]. The PC content in FP was 27 mg GAE/g. The chemical characteristics of the polysaccharides are shown in Table 5 .
The reagents, including the Folin and Ciocalteu’s phenol reagent and pectinase from Aspergillus niger (1.18 U/mg), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid was purchased from MP Biomedicals (Solon, OH, USA). Ethanol (95%, JSC Kirov Pharmaceutical Factory, Kirov, Russia), pyridine, boric acid, toluene and chloroform stabilized with ethyl alcohol (Vekton, Saint Petersburg, Russia), methanol (Merck, München, Germany, 99.5%), 3,5-dimethylphenol (Sigma Aldrich, USA, 99%), sodium borohydride (Sigma Aldrich, USA, 98.5%), Sulphuric acid (Sigma-tec, Moscow, Russia) were used.
The reagents, including the Folin and Ciocalteu’s phenol reagent and pectinase from Aspergillus niger (1.18 U/mg), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid was purchased from MP Biomedicals (Solon, OH, USA). Ethanol (95%, JSC Kirov Pharmaceutical Factory, Kirov, Russia), pyridine, boric acid, toluene and chloroform stabilized with ethyl alcohol (Vekton, Saint Petersburg, Russia), methanol (Merck, München, Germany, 99.5%), 3,5-dimethylphenol (Sigma Aldrich, USA, 99%), sodium borohydride (Sigma Aldrich, USA, 98.5%), Sulphuric acid (Sigma-tec, Moscow, Russia) were used.
6
Chromosome Preparation from Root Tips
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Root tips were collected from potted plants, pre-treated overnight at 4°C in 2mM 8hydroxyquinoline (BDH Chemicals), and then fixed in 3:1 (v/v) ethanol : acetic acid for 24 hr at 4°C (Bailey & Stace, 1992) . Next, root tips were digested in 10mM citrate buffer containing 25U/ml pectinase from Aspergillus niger (Sigma-Aldrich), 20U/ml cellulase from A. niger (Sigma-Aldrich) and 20 U/ml cellulase 'Onozuka R-10' from Trichoderma viride (Duchefa Biochemie). The digested roots were then dissected and squashed in 45% (v/v) acetic acid (Schwarzacher et al., 1989) . Chromosome preparations were preserved by quick-freezing on dry ice (Conger & Fairchild, 1953) , and mounted in VECTASHIELD® Mounting Medium with DAPI, before being observed and imaged on a Nikon Eclipse Ci fluorescence microscope.
7
Pectin Oligomer Characterization and ABA Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The POs with degree of polymerization in the range of 2 to 20 were obtained and characterized according to Vera-Guzmán et al. (2017) . Briefly, commercial pectin (Danisco Mexicana) was dissolved in water (1:45) and adjusted to pH 5. Then, it was hydrolyzed with a pectinase from Aspergillus niger (Sigma, USA) at 23°C for 3 h, stopping the reaction by autoclaving. The solution was dried using a Yamato ADL311S spray dryer to obtain the powdered mixture of POs. The degree of polymerization was determined by anion exchange chromatography with an amperometric pulse detector (HPAEC-PAD) (Dionex, Sunnyvale, CA) and a PD-10 column. The S-abscisic acid (ABA; ProTone ® SG) was purchased from Valent BioSciences Corp. (USA) and used as a control growth regulator substance as demonstrated in previous studies on table grapes (Peppi et al., 2008; Yamamoto et al., 2015) .
8
Somatic Chromosome Spreads from Root Tips
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromosomes of somatic metaphases were observed in root tip squashes obtained from seed germination. Seeds were placed on moisturized fi lter paper in Petri dishes and germinated at room temperature (about 25 ºC). Roots were collected when they reached 2 cm in length and were immediately pretreated with p-dichlorobenzene saturated solution for 3 h at room temperature and then fi xed in Farmer solution (absolute ethanol : glacial acetic acid, 3 : 1) for a minimum of 12 h at 4 ºC. Somatic chromosome spreads were prepared following the procedure described by Schwarzacher et al. (1980) . Root tips were macerated in 2% (m/v) cellulase (Onozuka R-10 from Trichoderma viridae; Serva, Heidelberg, Germany) plus 10% (v/v) pectinase (from Aspergillus niger; Sigma, St. Louis, Missouri, USA), dissolved in 40% glycerol in 0.01 mol/L citric acid/sodium citrate buffer, pH 4.8, at 37 ºC for 2 h, and then squashed in 45% acetic acid. After removal of the coverslip with CO 2 , slides were air dried, incubated for 1-2 days at room temperature, and then kept at 4 ºC until use.
9
Enzymatic Modification of Milk Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium hydroxide (1M), hydrochloric acid (1M), sodium azide, and pectinase from Aspergillus niger were purchased from Merck (Merk, Darmstadt, Germany). Casein micelle (CM) concentrate powder MC88 was kindly provided by Milei GmbH Germany and citrus pectin (highly methylated pectin classic CU 201) was obtained from Herbstreith & Fox (Herbstreith & Fox GmbH & Co. KG, Neuenbürg, Germany). The product Activa WM containing microbial transglutaminase was kindly provided by Ajinomoto Foods, Hamburg, Germany. Sodium dodecyl sulfate (SDS), ultra-pure Bis-Tris, calcium chloride, and all salts (purity >99% or analytical grade) for simulated milk ultrafiltrate (SMUF) preparation were purchased from VWR, Darmstadt, Germany. Milli-Q water was obtained from our lab.
10
Casein Micelle Concentrate Pectin Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Casein micelle concentrate (CMC) powder MC 80 was kindly provided by Milei GmbH Germany and citrus pectin (classic CU 201) was from Herbstreith & Fox (Herbstreith & Fox GmbH & Co. KG, Neuenbürg, Germany). Pectinase from Aspergillus niger, tri-sodium citrate, hydrochloric acid (1 M), sodium hydroxide (1 M), and sodium azide was from Merck (Merk, Darmstadt, Germany). Especially pure sodium dodecyl sulfate (SDS), ultra-pure BisTris, calcium chloride, and all salts (purity > 99% or analytical grade) for SMUF preparation were obtained from VWR, Radnor, USA. Milli-Q water was obtained from our lab.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!