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2 protocols using anti bmk1

1

Investigating Cellular Stress Signaling Pathways

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Anti-HA (Cat. number: 3724), anti-BMK1 (Cat. number: 3372), anti-phosho-ERK1/2 (Thr202/Tyr204) (Cat. number: 4376), anti-BNIP3 (Cat. number: 13795), anti-BNIP3L (Cat. number: 12396), anti-HIF1A (Cat. number: 14179), anti-Annexin V (Cat. number: 8555) and anti-ACTIN (Cat. number: 4967) antibodies were from Cell Signaling (Beverly, MA, USA). HA-tagged MEK5D-expressing vectors and BMK1 inhibitor (XMD8-92) were described in our previous study [12 (link)]. Sequence of shRNA (MISSION® shRNA Library, sigma, USA) used in this study was described in Supplementary Table S4. pLKO.1-scramble shRNA was used as shRNAi control.
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2

Antibody-based signaling pathway analysis

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Anti-HA (Cat. number: 3724), anti-BMK1 (Cat. number: 3372), anti-ERK1/2 (Cat. number: 9102), anti-phosho-ERK1/2 (Thr202/Tyr204) (Cat. number: 4376), anti-phosho-Jun (Ser63) (Cat. number: 2361), anti-Jun (Cat. number: 2315), anti-SRC (Cat. number: 2109), anti-phosho-SRC (Tyr416) (Cat. number: 2101) and anti-ACTIN (Cat. number: 4967) antibodies were from Cell Signaling (Cell Signaling, Beverly, MA USA). pCDNA3 HA-tagged MEK5D vectors was described in our previous study13 (link). pGIPZ-BMK1 encoding shRNA against human BMK1, and pGIPZ-nontarget shRNAs were from Open Biosystems as described in the previous study26 (link).
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