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P jak1 tyr1034 1035

Manufactured by Cell Signaling Technology
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The P-JAK1(Tyr1034/1035) product is a phospho-specific antibody that recognizes JAK1 protein when phosphorylated at tyrosine residues 1034 and 1035. This antibody can be used to detect and analyze the activation status of JAK1 in various cell and tissue samples.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (3 mentions) Mesothelin, by Cell Signaling Technology (2 mentions) P erbb2, by Cell Signaling Technology (2 mentions) Igf 1rβ, by Cell Signaling Technology (2 mentions) Tgf β, by Cell Signaling Technology (2 mentions) Furin, by Santa Cruz Biotechnology (2 mentions) Jak2 3230s, by Cell Signaling Technology (2 mentions) P jak2 tyr1008, by Cell Signaling Technology (2 mentions) P tyk2 tyr1054 1055, by Cell Signaling Technology (2 mentions) Recombinant human neuregulin β 1 nrg1, by Thermo Fisher Scientific (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) P erbb3, by Cell Signaling Technology (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Dmem media, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Skov3, by American Type Culture Collection (2 mentions) P jak2 tyr1007 1008, by Cell Signaling Technology (1 mentions) P stat3 tyr705, by Cell Signaling Technology (1 mentions) Stat3, by Proteintech (1 mentions)

Close spelling variants of this product

P-JAK1 (41 citations)

4 protocols using p jak1 tyr1034 1035

1

Molecular Mechanisms of JAK/STAT Signaling

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The following antibodies were used: anti-CEP55 (#23891-1-AP), MMP2 (#66366-1-Ig), MMP9 (#10375-2-AP), STAT3 (#60199-1-Ig), JAK1 (#66466-1-Ig), JAK2 (#17670-1-AP), and Actin (#60008-1-Ig) (Proteintech Group, Chicago, IL, USA); and p-JAK1 Tyr1034/1035 (#74129), p-JAK2 Tyr1007/1008 (#3771), and p-STAT3 Tyr705 (#9145) (Cell Signaling Technology, Beverly, MA, USA). The biological reagent IL-6 was acquired from Sigma Chemical (St. Louis, MO, USA). JAK2 inhibitor XL019 (#S7036), STAT3 inhibitor C188-9 (#S8605) was from Selleck (Shanghai, China).
Li M., Gao J., Li D, & Yin Y. (2018). CEP55 Promotes Cell Motility via JAK2–STAT3–MMPs Cascade in Hepatocellular Carcinoma. Cells, 7(8), 99.
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2

Lung Adenocarcinoma Cell Lines and IL-22 Signaling

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The human lung adenocarcinoma (AC) cell line A549 was bought from the National Collection of Authenticated Cell Cultures (Shanghai, China). The human lung AC cell line PC-9 was received as a gift from Professor Caicun Zhou (Shanghai Pulmonary Hospital, Shanghai, China). In the present study, all cells were cultured in RPMI-1640 (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco BRL Co., Ltd., Houston, TX, USA) at 37°C in 5% CO2. The human recombinant IL-22 was bought from PeproTech (Rocky Hill, NJ, USA). The IL-22 was dissolved in dimethyl sulfoxide (DMSO; St. Louis, MO, USA) at a concentration of 10 μg/mL and stored at -20°C. The final concentration for the DMSO in the whole experiment was maintained at less than 0.1%. The p44/42 MAPK (ERK1/2), p-p44/42 MAPK (p-ERK1/2, T202/Y204), P38, p-P38 MAPK (Thr180/Tyr182), AKT, p-AKT (Thr308), JAK1, p-JAK1 (Tyr1034/1035), JAK2, p-JAK2 (Tyr1007/1008), STAT3 and p-STAT3 (Tyr705) antibodies, and GAPDH were purchased from Cell Signaling Technology (CST, Danvers, MA, USA).
Yao Y., Yang G., Lu G., Ye J., Cui L., Zeng Z., Chen J, & Zhou J. (2022). Th22 Cells/IL-22 Serves as a Protumor Regulator to Drive Poor Prognosis through the JAK-STAT3/MAPK/AKT Signaling Pathway in Non-Small-Cell Lung Cancer. Journal of Immunology Research, 2022, 8071234.
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3

Characterization of Ovarian Cancer Cell Lines

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Ovarian cancer cell line OVCAR5 cells were purchased from the National Cancer Institute’s cell line repository. SKOV3, cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and HeyA8 cells were received from the characterized cell line core at MD Anderson Cancer Center. Cells were cultured in DMEM media (GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). ID8 cells (a kind gift from Dr. Weiguo Cui) were cultured in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% FBS, 1% penicillin/streptomycin, 1% insulin (5 g/mL), 5 g/mL transferrin, 5 ng/mL sodium selenite (1X ITS; Sigma Cat. No. I3146). We obtained, ERBB2 (#2165), p-ERBB2 (Tyr1248)(#2247), ERBB3 (#12708), p-ERBB3 (Tyr1289), IGF1R-β (#3027), GAPDH (#5174), mesothelin (#99966S), TGF-β (#3711), STAT3 (#9139), p-STAT3 (Tyr705)(#9145), p-STAT3 (Ser727)(#34911), JAK1 (#3344S), p-JAK1(Tyr1034/1035) (#74129S), JAK2 (3230S), p-JAK2 (Tyr1008) (#8082S) TYK2 (#14193S) and p-TYK2 (Tyr1054/1055) (#68790S) from Cell Signaling Technology (Danvers, MA), Furin (#sc-133142) was obtained from Santa Cruz Biotechnology. Recombinant Human Neuregulin β−1 (NRG1), was purchased from PeproTech, Cat# 100–03.
Chen C., Gupta P., Parashar D., Nair G.G., George J., Geethadevi A., Wang W., Tsaih S.W., Bradley W., Ramchandran R., Rader J.S., Chaluvally-Raghavan P, & Pradeep S. (2020). ERBB3-induced furin promotes the progression and metastasis of ovarian cancer via the IGF1R/STAT3 signaling axis. Oncogene, 39(14), 2921-2933.
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4

Characterization of Ovarian Cancer Cell Lines

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Ovarian cancer cell line OVCAR5 cells were purchased from the National Cancer Institute’s cell line repository. SKOV3, cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and HeyA8 cells were received from the characterized cell line core at MD Anderson Cancer Center. Cells were cultured in DMEM media (GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). ID8 cells (a kind gift from Dr. Weiguo Cui) were cultured in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% FBS, 1% penicillin/streptomycin, 1% insulin (5 g/mL), 5 g/mL transferrin, 5 ng/mL sodium selenite (1X ITS; Sigma Cat. No. I3146). We obtained, ERBB2 (#2165), p-ERBB2 (Tyr1248)(#2247), ERBB3 (#12708), p-ERBB3 (Tyr1289), IGF1R-β (#3027), GAPDH (#5174), mesothelin (#99966S), TGF-β (#3711), STAT3 (#9139), p-STAT3 (Tyr705)(#9145), p-STAT3 (Ser727)(#34911), JAK1 (#3344S), p-JAK1(Tyr1034/1035) (#74129S), JAK2 (3230S), p-JAK2 (Tyr1008) (#8082S) TYK2 (#14193S) and p-TYK2 (Tyr1054/1055) (#68790S) from Cell Signaling Technology (Danvers, MA), Furin (#sc-133142) was obtained from Santa Cruz Biotechnology. Recombinant Human Neuregulin β−1 (NRG1), was purchased from PeproTech, Cat# 100–03.
Chen C., Gupta P., Parashar D., Nair G.G., George J., Geethadevi A., Wang W., Tsaih S.W., Bradley W., Ramchandran R., Rader J.S., Chaluvally-Raghavan P, & Pradeep S. (2020). ERBB3-induced furin promotes the progression and metastasis of ovarian cancer via the IGF1R/STAT3 signaling axis. Oncogene, 39(14), 2921-2933.
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