Vanquish flex uplc

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Vanquish Flex UPLC is a high-performance liquid chromatography (HPLC) system designed for analytical applications. It offers precise and reliable separation of complex mixtures, enabling efficient and reproducible sample analysis. The system features advanced technology and robust engineering to support a wide range of analytical needs.

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Lab products found in correlation

Acquity uplc beh c18 column, by Waters Corporation (2 mentions) Q exactive high resolution mass spectrometer, by Thermo Fisher Scientific (1 mentions) Csh c18, by Waters Corporation (1 mentions) Tmt reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Vanquish UPLC (27 citations) Vanquish Flex (26 citations) Vanquish Flex UPLC system (4 citations)

4 protocols using vanquish flex uplc

Metabolomic Analysis of Chinese Medicines

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Briefly, 100 µL of blood samples were prepared in 1.5 mL EP tubes on ice and added with 400 µL of ice methanol solution, swirled for 30 s, stored at −20°C for 30 min and centrifuged for 15 min (20,000 rcf, 4°C). The supernatant was collected and centrifuged under the same conditions. The supernatant after two centrifugations was transferred to the sample vial for UPLC-Q-Orbitrap-MS detection.
Vanquish Flex UPLC (Thermo Fisher Scientific, Bremen, Germany) with UPLC column ACQUITY UPLC T3 (100 mm*2.1 mm, 1.8 µm, Waters, UK) was used for the analysis of Chinese medicine samples. The mobile phase system was composed of 5 mmol/L ammonium acetate +5 mmol/L acetic acid (A) and LC-MS acetonitrile (acetonitrile) (B). The flow rate was controlled at 0.35 mL/min, and the gradient program was 0–1 min, 1%B; 1–9.5 min, 1–99%B; 9.5–11.5 min, 99–99%B; 11.5–12 min, 99–1%B; and 12–15 min, 1%B. The column temperature was 35°C. Q-Exactive high-resolution mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) was used to acquire positive and negative ion mode mass spectrometry data. The optimum conditions of ion source were as follows: source temperature, 350°C; sheath gas, 12 Arb; spray voltage, 3.80 kV (positive ion mode), −3.50 kV (negative ion mode); and auxiliary gas, 45 Arb.

Gao H., Yuan Z., Liang H, & Liu Y. (2024). Integrating UPLC-Q-Orbitrap MS with serum pharmacochemistry network and experimental verification to explore the pharmacological mechanisms of Cynanchi stauntonii rhizoma et radix against sepsis-induced acute lung injury. Frontiers in Pharmacology, 15, 1261772.

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SCFA Analysis by UPLC-QE-MS

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A Thermo Scientific Vanquish Flex UPLC coupled Q Exactive (QE) system was used to analyze derivatized SCFAs. The QE was coupled to a universal ion source with Ion Max heated electrospray ionization (HESI-II) probe. The PRM mode was used for qualitative determination of SCFAs, while t-SIM mode was used for quantitative analysis. The mass spectra were recorded in negative ion mode. The QE mass parameters were as follows: sheath gas flow rate 10 mL/min, spray voltage 4000V, capillary temperature 320 °C, resolution 70000 FWHM, Automatic gain control target 5 × 10⁴ (t-SIM), 2 × 10⁵ (PRM), and 1 × 10⁶ (tune method).
Separation was performed with Waters CSH C18 (2.1×100mm, 1.7μm) column. The samples were introduced into LC using an auto-sampler (5μL, maintained at 6 °C) in a two-mixture mobile phase: mobile phase A was composed of water (0.1% formic acid), and B was acetonitrile (0.1% formic acid). The initial condition of 15% B held for 2 min and then linearly ramped to 55% B by 9 min, then to 100% B by 0.1min and held for 1 min. The final value was then decreased linearly to 15% B by 0.1 min and held for 4 min. The mobile phase flow rate was held constant at 0.35mL min⁻¹. According to the FDA guideline for bioanalytical method validation [34 ], the calibration curve, accuracy, precision, recovery and stability were validated as follow.

Chen L., Sun X., Khalsa A.S., Bailey M.T., Kelleher K., Spees C, & Zhu J. (2021). Accurate and Reliable Quantitation of Short Chain Fatty Acids from Human Feces by Ultra High-Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS). Journal of pharmaceutical and biomedical analysis, 200, 114066.

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Protein Labeling and Fractionation

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We added TEAB (triethylammonium bicarbonate buffer) to 100 μg protein samples to 100 mM. Then, we added TCEP (tris (2-carboxyethyl) phosphine) to 10 mM and let the reaction take place for 40 min under dark conditions. Then, 6 times the acetone was added to the sample and settled for 4 h at −20 °C. The sediment was collected after centrifugation for 20 min at 10,000× g and 100 µL 100 mM TEAB was added. Finally, samples were digested with a 1:50 trypsin-to-protein mass ratio overnight at 37 °C. One unit of TMT reagent was added to a 100 μg sample for 2 h at room temperature. Then, hydroxylamine was added to react for 30 min at room temperature. The concentrated samples were fractionated into fractions by Vanquish Flex UPLC (Thermo Fisher Scientific, Pierce, CO, USA) with ACQUITY UPLC BEH C18 Column (1.7 µm, 2.1 mm × 150 mm, Waters, Milford, USA) to increase proteomic depth.

Wang L., Li A., Fang J., Wang Y., Chen L., Qiao L, & Wang W. (2023). Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation of Enterococcus faecium. International Journal of Molecular Sciences, 24(14), 11822.

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Multiplexed Proteome Quantification by TMT

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TEAB (triethylammonium bicarbonate buffer) and TCEP (tris (2-carboxyethyl) phosphine) were mixed with samples and incubated for 60 min at 37 °C. Subsequently, IAM (Iodoacetamide) at a final concentration of 40 mM was added, and the mixture stood for 40 min at a temperature in dark conditions. After centrifugation for 20 min at 10,000× g, the precipitate was collected and dissolved by TEAB. Finally, the mixture was hydrolyzed overnight with trypsin at 37 °C.
Peptides were labeled with TMT reagent (A44522, Thermo Fisher Scientific, MA, USA). The samples were labeled, pooled, desalted, and vacuum-dried. The pooled samples were fractionated by ACQUITY UPLC BEH C18 Column (1.7 µm, 2.1 × 150 mm, Waters, Milford, MA, USA) on Vanquish Flex UPLC (Thermo Fisher Scientific, Waltham, MA, USA).

Ding N., Meng H., Wu C., Yokoyama W., Hong H., Luo Y, & Tan Y. (2023). Whey Protein Hydrolysate Renovates Age-Related and Scopolamine-Induced Cognitive Impairment. Nutrients, 15(5), 1228.

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