Log In Sign up
The largest database of trusted experimental protocols

Rhil 12 p70

Manufactured by Thermo Fisher Scientific
Visit Supplier

RhIL-12 p70 is a recombinant human interleukin-12 subunit p70 protein. It is a cytokine that plays a role in the regulation of immune responses.

Automatically generated - may contain errors

Lab products found in correlation

Htgf β, by R&D Systems (1 mentions) Dynabeads human t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions) Human na ve cd4 t cell isolation kit, by STEMCELL (1 mentions) Rhil 10, by Thermo Fisher Scientific (1 mentions) Rhil 23, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

RhIL-12 (11 citations)

2 protocols using rhil 12 p70

1

Naïve Human T Cell Differentiation under Cytokine Skewing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve human cells were isolated by using Human Naïve CD4+ T Cell isolation kit (Stemcell Technologies, 17555) from peripheral blood mononuclear cells (PBMCs) after Ficoll-Paque gradient (GE Healthcare Systems). Naïve human CD4+ T cells were incubated with Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher, 11161D) for 3 days in R10 under the following skewing conditions: a) rhIL-23 (25 ng/ml, PeproTech); b) rhIL-23 (25 ng/ml, PeproTech) plus rhIL-10 (10 ng/ml PeproTech); c) rhIL-12 p70 (1 ng/ml, PeproTech) and d) rhIL-12 p70 (1 ng/ml, PeproTech) plus rhIL-10 (10 ng/ml PeproTech). The former conditions were cultured in the presence/absence of hTGF-β (5 ng/ml) (R&D Systems).
Chaurio R.A., Anadon C.M., Costich T.L., Payne K.K., Biswas S., Harro C.M., Moran C., Ortiz A.C., Cortina C., Rigolizzo K.E., Sprenger K.B., Mine J.A., Innamarato P.P., Mandal G., Powers J.J., Martin A., Wang Z., Mehta S., Perez B.A., Li R., Robinson J., Kroeger J.L., Curiel T.J., Yu X., Rodriguez P.C, & Conejo-Garcia J.R. (2022). TGF-β-mediated silencing of the genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures. Immunity, 55(1), 115-128.e9.
+ Open protocol
+ Expand
2

Stimulation of Naive CD4 T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naive CD4 T cells of healthy controls were purified using MojoSort (BioLegend), stimulated at a concentration of 5 µl/106 cells/ml anti-CD3/CD28/CD2 beads (STEMCELL Technologies SARL) in X-VIVO 15 medium (Lonza Sales Ltd.) + 5% antibody (AB) serum (Sigma-Aldrich) and 100 ng/ml rhIL-12p70 (PeproTech) until further use.
Kögl T., Chang H.F., Staniek J., Chiang S.C., Thoulass G., Lao J., Weißert K., Dettmer-Monaco V., Geiger K., Manna P.T., Beziat V., Momenilandi M., Tu S.M., Keppler S.J., Pattu V., Wolf P., Kupferschmid L., Tholen S., Covill L.E., Ebert K., Straub T., Groß M., Gather R., Engel H., Salzer U., Schell C., Maier S., Lehmberg K., Cornu T.I., Pircher H., Shahrooei M., Parvaneh N., Elling R., Rizzi M., Bryceson Y.T., Ehl S., Aichele P, & Ammann S. (2024). Patients and mice with deficiency in the SNARE protein SYNTAXIN-11 have a secondary B cell defect. The Journal of Experimental Medicine, 221(7), e20221122.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!