Hep 2 cells

Manufactured by Thermo Fisher Scientific

Sourced in United States

Hep-2 cells are a type of human epithelial cell line derived from a carcinoma of the larynx. They are commonly used as a model system in cell biology and virology research. The core function of Hep-2 cells is to provide a standardized and reproducible cell line for various experimental applications.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Newborn calf serum, by Thermo Fisher Scientific (1 mentions) Acanthamoeba castellanii, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

HEPES (4332 citations) Hep-2 (13 citations)

3 protocols using hep 2 cells

Hep-2 Cell Culture and Lentiviral Transduction

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We purchased Hep-2 cells from Shanghai Phytobiotechnology Company (Shanghai, China). Our team cultured Hep-2 cells in DMEM (Gibco, GrandIsland, NY, USA) medium with 10% FBS (BIOEXPLORER, collected in South America) and 1% streptomycin/penicillin antibiotics under 37 °C and 5% CO₂.
We constructed lentivirus constructs overexpressing hsa_circ_0013561 (sh-circ0013561) and negative control (NC) vectors. Stable Hep-2 knockdown cells were generated after lentivirus infection. After cell passaging, RNA was extracted to verify infection efficiency.

Tian K., Zheng L., Yuan T., Chen X., Chen Q., Xue X., Huang S., He W., Jin M, & Zhang Y. (2024). The circRNA hsa-circ-0013561 regulates head and neck squamous cell carcinoma development via the miR-7-5p/PDK3 axis. Cancer Cell International, 24, 91.

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Culturing Laryngeal Carcinoma Hep-2 Cells

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Laryngeal carcinoma Hep-2 cells (ATCC) were cultured in RPMI-1640 medium (GIBCO/BRL, Gaithersburg, Md, USA) supplemented with 10% new-born calf serum (GIBCO/BRL) with 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in a homeothermic incubator with a 5% CO₂ atmosphere. The medium was changed every two or three days.

Wang Q., Cao W., Su Q., Liu Z, & Zhang L. (2014). Clusterin silencing inhibits proliferation and reduces invasion in human laryngeal squamous carcinoma cells. World Journal of Surgical Oncology, 12, 124.

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Culturing Acanthamoeba and Human Cells

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Amoeba and cell culture Acanthamoeba castellanii (strain ATCC30011) was obtained from the American Type Culture Collection (ATCC). Trophozoites were cultured axenically in peptone-yeast-glucose (PYG) medium. Cultures were incubated at 26 °C and trophozoites were harvested in the late log phase after subculture for 72 h. HEp-2 cells purchased from ATCC were cultured in EMEM (Gibco) supplemented with 10% fetal bovine serum (FBS) (Thermo), 100 U/ml penicillin and 100 µg/ml streptomycin. Human corneal epithelial cells (HCECs) purchased from Bioleaf (Shanghai, China) were grown in DMEM (Gibco) with 10% FBS. Both cells were cultured in a 37 °C incubator with 5% CO2.

Wang Z., Wu D., Tachibana H., Feng M., & Cheng X. (2020). Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3.

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