Pcmv5 myc

Manufactured by Qiagen

PCMV5-Myc is a plasmid vector used for the expression of recombinant proteins in mammalian cells. It contains the human cytomegalovirus (CMV) immediate-early promoter, which drives high-level expression of the target gene. The vector also includes the c-Myc epitope tag, which can be used for detection and purification of the expressed protein.

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Lab products found in correlation

Pegfp n3, by Takara Bio (2 mentions) Pcdna3.1 v5 his, by Thermo Fisher Scientific (2 mentions) Pclxsn gfp, by Takara Bio (2 mentions) Glutathione sepharose beads, by Cytiva (2 mentions) Hiload 16 60 superdex 200 column, by Cytiva (2 mentions) Pgex 5x 3, by Cytiva (2 mentions)

2 protocols using pcmv5 myc

Cloning and Purification of rMap7D2 Protein

Cited 1 time

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Based on the information of DDBJ/EMBL/GenBank accession number NM_001289778.1, oligonucleotide primers (5′-ATGTCGACATGGAGCGCAGCGGTGGGAACGGCG-3′ and 5′-ATGTCGACTCAACAGAAGGTGTTCAGCGTAGTTTC-3′) were designed, and rat Map7d2 (rMap7d2) cDNA was obtained by PCR using rat cDNA as a template. Expression vectors for rMap7d2 were constructed in pCMV5-Myc (Nakanishi et al, 1997 (link)), pQE9 (QIAGEN), pGEX-5X-3 (Cytiva), pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al, 2005 (link)), and pEGFP-N3 (Clontech). GST-fused proteins were expressed in Escherichia coli and purified using glutathione-Sepharose beads (Cytiva), respectively. GST-rMap7D2 (full length) was further purified by gel filtration using a HiLoad 16/60 Superdex 200 column (Cytiva).

Kikuchi K., Sakamoto Y., Uezu A., Yamamoto H., Ishiguro K.I., Shimamura K., Saito T., Hisanaga S.I, & Nakanishi H. (2022). Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells. Life Science Alliance, 5(8), e202201390.

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Cloning and Expression of rat Map7d2

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Based on the information of DDBJ/EMBL/GenBank accession number XM_228973, oligonucleotide primers (5′-ATGTCGACATGGAGCGCAGCGGTGGGAACGGCG-3′ and 5-ATGTCGACTCAACAGAAGGTGTTCAGCGTAGTTTC-3′) were designed, and rat Map7d2 (rMap7d2) cDNA was obtained by PCR using rat cDNA as a template. Expression vectors for rMap7d2 were constructed in pCMV5-Myc (Nakanishi 1997) , pQE9 (Qiagen), pGEX-5X-3 (Cytiva), pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al 2005) , and pEGFP-N3 (Clontech). GST-fused proteins were expressed in Escherichia coli and purified using glutathione-Sepharose beads (Cytiva), respectively. GST-rMap7D2 (full length) was further purified by gel filtration using a HiLoad 16/60 Superdex 200 column (Cytiva).

Kikuchi K., Sakamoto Y., Uezu A., Yamamoto H., Ishiguro K., Shimamura K., Saito T., Hisanaga S., & Nakanishi H. (2021). Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells.

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