Tumor and liver tissue were fixed in ALFAC solution (85% ethanol, 10% formaldehyde, 5% glacial acetic acid) at room temperature for 16 h following the routine dehydration for paraffin embedding. Samples were sectioned at 5 µm thickness and stained with hematoxylin and eosin. Analysis was performed by optical microscopy in 3 or 4 slides per group. The evaluated parameters were apoptosis (Grade 0: absent; Grade I: discrete; Grade II: moderate), inflammatory infiltration (Grade 0: absent; Grade I: discrete; Grade II: moderate; Grade III: high) and coagulative necrosis (Grade 0: absent; Grade I: present in 1–20% of the histological area; Grade II: 21–50%; Grade III: 51–80%; Grade IV: 81–100%). Images were acquired microscopically using Axio Imager Z2 epifluorescence (Carl Zeiss, Germany), equipped with an automated slide scanner from MetaSystems (MetaViewer version. 2.0.100, Germany).
Axio imager z2 epifluorescence
Manufactured by Zeiss
Sourced in Germany
The Axio Imager Z2 is an epifluorescence microscope designed for high-resolution imaging and analysis. It features a stable and vibration-free stand, a motorized focusing drive, and a wide range of optics and illumination options to support various fluorescence-based applications.
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Lab products found in correlation
4 protocols using axio imager z2 epifluorescence
1
Histopathological Evaluation of Tumor and Liver Tissue
2
Visualizing Microbial Motility Dynamics
3
Visualizing Microbial Motility Dynamics
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To test motility on plates, 3μl of log-phase cells were spotted onto semi-solid agar (1% Tryptone, 0.5% Sodium Chloride, 0.25% Agar), and grown 7 hours at 37°C under green (525nm) or red light (650nm). To observe cell motility by microscopy, 10 μl of cell culture was mounted on a glass slide by placing the drop between two #1.5 coverslips, then covering it with a third #1.5 coverslip. Videos of motile and non-motile cells were recorded using the streaming mode of a Hamamatsu Orca-Flash4.0 sCMOS camera mounted on a Zeiss Axio Imager Z2 epifluorescence microscope, viewed through a Plan-Apochromat, 20x/0.18 Oil Ph1 objective. Phase contrast and fluorescence movies (filter 63HE) consisted of 500 image frames, acquired continuously with 30ms exposure times, and were acquired independently but in rapid succession, focused on the same field. The TrackMate plugin for ImageJ was used to analyze these movies in order to track cell motility.
4
Mitochondrial Membrane Potential Imaging
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TMRE staining: Tetramethylrhodamine, ethyl ester, perchlorate is a dye that accumulates in intact, functional mitochondria. 1-day-adult animals were grown at 20°C in the presence of 150 nM TMRE for 24 h. Stained and washed worms were immobilized with levamisole before mounting on 2% agarose pads for microscopic examination with a Zeiss AxioImager Z2 epifluorescence microscope. Images were acquired under the same exposure. Average pixel intensity values were calculated by sampling images of different animals. We calculated the mean and maximum pixel intensity for each animal using the Fiji software (https://fiji.sc ). For each experiment, at least 35 animals were examined for each strain/condition. Each assay was repeated at least three times. We used the Prism software package (GraphPad Software) for statistical analyses.
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