Histologic and morphologic analysis was performed as previously described [19 (link)]. Histochemical staining was performed with hematoxylin/eosin and sirius red. Immunohistochemical staining was performed using primary antibodies against p-Smad3 (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), α-SMA (1:500; Sigma, St. Louis, MO, USA), PAI-1 (1:500; BD Biosciences, San Jose, CA, USA), and type 1 collagen (1:500; Abcam, Cambridge, UK), followed by horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin G secondary antibodies (Dako, Glostrup, Denmark). Quantification of renal fibrosis was measured as previously described [19 (link)].
Horseradish peroxidase conjugated anti mouse or anti rabbit immunoglobulin g secondary antibodies
Manufactured by Agilent Technologies
Sourced in United Kingdom
Horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin G secondary antibodies are enzyme-linked antibodies used in various immunoassay techniques. They function as detection reagents, binding to primary antibodies directed against mouse or rabbit antigens, and enabling visualization or quantification through an enzymatic reaction.
Automatically generated - may contain errors
2 protocols using horseradish peroxidase conjugated anti mouse or anti rabbit immunoglobulin g secondary antibodies
1
Histochemical and Immunohistochemical Analysis of Renal Fibrosis
2
Kidney Tissue Histological Analysis
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Kidney tissues were fixed with 4% paraformaldehyde, embedded in paraffin, and cut into 4 μm-thick sections. To assess histological morphology, immunohistochemical staining was performed using indicated antibodies and horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin G secondary antibodies (Dako). The stained sections were imaged with a Nikon Eclipse Ni-U microscope. The quantitative analysis of the stained sections was performed using imageJ software (National Institutes of Health).
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