Aortas were obtained from Sprague-Dawley rats, and VSMCs were grown from aortic explants and used at passages 3 to 6. Cells were treated with different conditions when they reached 75–85% confluence: (1) AngII (Sigma) at 1 × 10−6 mol l−1 (determined by prestudy) for 48 h; (2) emodin (Pfizer, Groton, CT, USA) 10, 40 and 80 μmol l−1 for 48 h; (3) miR-126 mimic and Lipofectamine 2000 reagent for 6 h, then LiCl (20 mmol l−1) and/or AngII (1 × 10−6 mol l−1) for 48 h; and (4) miR-126 inhibitor and Lipofectamine 2000 reagent (Invitrogen, Milan, Italy) for 6 h, then AngII (1 × 10−6 mol l−1) for 48 h. The morphology of the cultured cells was observed by electron microscopy. Each treatment was performed at least 3 times.
Angii
Manufactured by Thermo Fisher Scientific
Sourced in Italy, United States
AngII is a laboratory reagent used for research purposes. It is an angiotensin II peptide that can be utilized in various biochemical and cell-based assays. The core function of AngII is to serve as a tool for studying signaling pathways, receptor binding, and other relevant biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Ang 2, by Merck Group (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
Pcr primers and reagents, by Thermo Fisher Scientific (1 mentions)
Tunel antibody, by Abcam (1 mentions)
Low speed centrifuge, by Beckman Coulter (1 mentions)
Pentobarbital sodium, by Merck Group (1 mentions)
Phosphatase inhibitor, by Roche (1 mentions)
3 protocols using angii
1
VSMC Treatments and Morphological Changes
2
Angiotensin II-Induced Hypertension Model
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Procell Life Science and Technology Co. Ltd (Wuhan, China) provided hVSMCs. One percent penicillin-streptomycin solution (P/S) and 10% fetal bovine serum (FBS) were added to Dulbecco's modified eagle medium (DEME, Gibco, New York) for cell culture. Cells were incubated within the humid incubator under 37°C and 5% CO 2 conditions. For cell treatment, Ang II-induced hypertension was established after hVSMCs treated with 1 μM Ang II (Invitrogen, California, USA) at 0, 3, 6, 12, 24, and 48 h. The viability of 24 h hVSMCs was about 60%, which is suitable for follow-up functional research. Therefore, Ang II-induced hypertension model was established after hVSMCs treated with 1 μM Ang II for 24 h.
3
Sodium Ferulate Attenuates Cardiac Hypertrophy
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Experimental animals are 72 male C57BL/6J mice at the age of 6–8 weeks supplied by Experimental Animal Center. Drugs are as follows: sodium ferulate (SF) injection solution (Shandong Fangming Pharmaceutical Group, China), AngII (Invitrogen, USA), TBHQ (Selleck, USA), and pentobarbital sodium (Merck, USA). Instruments are as follows: PUMP 11 Elite Injection Pump (Harvard, USA), CODA Noninvasive Blood Pressure Measurement System (Kent scientific Corporation, USA), VEVO2100 Ultrasonic Image System for Small Animals (Visual Sonic, Canada), Microscope (OLYMPUS, Japan), Microplate Reader (Biotek, USA), PCR Instrument (Thermo, USA), Low-Speed Centrifuge (Beckman Coulter, USA), and Gel DoxTM XR + Gel Imaging System (Bio-Rad, USA). Reagents are as follows: RNA extraction and PCR reagents (Roche, Switzerland), TUNEL antibody (Abcam, USA), phosphatase inhibitor (Roche, Switzerland), atrial natriuretic peptide (ANP)/transforming growth factor-β (TGF-β)/Collagen III (Col III)/bcl-2/bax/caspase-3/ERK/p-ERK/p38MAPK/p-p38MAPK/JNK/p-JNK/β-actin antibodies (Cell Signaling Technology, USA), BCA quantitation and Western Blot reagents (Invitrogen, USA), and PCR primers and reagents (Invitrogen, USA). The sequences were as shown in Table 1 .
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