Lipids were extracted from liver tissue using chloroform:methanol mixture (2:1 v/v) (40 (link)). enzymatic assay kits were used to measure hepatic and plasma concentrations of triglyceride (Wako Diagnostics). Plasma AST and ALT activities were measured by an enzymatic assay kit (Thermo Fisher Scientific). Plasma insulin and Fgf21 concentrations were determined using commercially available mouse ELISA kits from Crystal Chem and R&D Systems, respectively, according to the manufacturer's specification. Protein concentrations were determined using a bicinchoninic acid assay (Thermo Fisher Scientific).
Enzymatic assay kit
Manufactured by Thermo Fisher Scientific
Sourced in Canada
Enzymatic Assay Kits are laboratory products designed to measure the activity or concentration of specific enzymes in a sample. These kits provide a standardized and reliable method for quantifying enzyme levels, which is essential for various applications in biochemistry, molecular biology, and clinical research.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using enzymatic assay kit
1
Liver Lipid Profiling Protocol
2
Hepatic Biomarker Measurement in Mice
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Multiple biomarkers represented for hepatic function including serum plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) of these mice were measured using enzymatic assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
3
Serum ALT and AST Enzymatic Assay
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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured using Enzymatic Assay Kits (Thermo Scientific, Waltham, MA).
4
Enzymatic Assay for Lipid Profiling
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Enzymatic assay kits were used to measure total cholesterol (Thermo Fisher, Ottawa, ON, Canada), unesterified cholesterol, and HDL-cholesterol (FujiFilm Medical Systems, formerly Wako Diagnostics, Richmond, VA) following the manufacturer’s instructions. Cholesteryl ester and non-HDL cholesterol values were determined by subtraction of unesterified cholesterol or HDL-cholesterol (respectively) from total cholesterol.
5
Serum Enzyme Assay Protocol
6
Lipoprotein Effects on Apoptosis
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Plasma was fractioned by gel filtration fast-protein liquid chromatography using an AKTA system with a Tricorn Superose 6 HR10/300 column (GE Healthcare Life Sciences, Baie d'Urfe, QC, Canada). Enzymatic assay kits were used to measure total cholesterol (Thermo Fisher Scientific, Ottawa, ON, Canada). IL-6 and tumor necrosis factor (TNF)-α levels were measured by ELISA (Biolegend, San Diego, CA, USA), following the manufacturer's instructions. ] and cells were cultured for 24 h. At the start of each experiment, the medium was changed to fresh Medium 1 containing 3% newborn calf serum (NCS, Life Technologies Inc., Burlington, ON, Canada), or 3% NCLPDS without or with supplementation of different concentrations of human HDL or LDL, without or with addition of apoptosis inducing agents as indicated. Apoptosis inducing agents and concentrations used were: tunicamycin (10 μg/ml); thapsigargin (5 μM); staurosporine (0.3 μM); oxidized (ox) LDL (100 μg protein/ml). Controls contained an equivalent amount of vehicle (0.1% DMSO for tunicamycin, thapsigargin or staurosporine; or saline for oxLDL).
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