Acetonitrile hplc s grade

EAI045 (>99.4%, molecular weight 383.40 g/mol) and PIA (>95%, molecular weight 301.27 g/mol) originated from Carbosynth (Compton, Berkshire, UK). PLX4720 (>99%, molecular weight 413.83 g/mol) was obtained from Selleck Chemicals (Houston, TX, USA). Water (ULC–MS grade), methanol (HPLC grade) and acetonitrile (HPLC‐S grade) were obtained from Biosolve (Valkenswaard, The Netherlands). Formic acid of analytical grade was supplied by Merck (Darmstadt, Germany). Water, purified by reversed osmosis was available at the research institute and used for all other purposes. Both pooled blank human lithium–heparin plasma and pooled female mouse lithium–heparin plasma were provided by Sera Laboratories (Haywards Heath, UK). Blank mouse tissue homogenates were prepared by mixing the whole organ with ice‐cold 2% (w/v) bovine serum albumin in Milli‐Q water followed by homogenization using the FastPrep‐24™ 5G instrument (M.P. Biomedicals, Santa Ana, CA, USA) for 1 min. Different appropriate volumes were used for different organs (1 ml for brain, spleen and lung; 2 ml for kidney; and 3 ml for liver and SIWC). All matrices for analysis were stored at −30°C.

Cystine, NEM and sulfosalicylic acid were purchased from Sigma‐Aldrich (Zwijndrecht, The Netherlands). Deuterated internal standard Cystine‐D4 was purchased from Cambridge Isotope Laboratories (Tewksbury, https://en.wikipedia.org/wiki/Massachusetts, USA). Acetonitrile (HPLC‐S grade) was obtained from Biosolve (Valkenswaard, The Netherlands). Formic acid was purchased from Merck (Darmstadt, Germany). Ultrapure water was home‐purified on a Milli‐Q® Advantage A10 Water Purification Systems (Merck, The Netherlands).