Megamix
Megamix is a laboratory mixer designed for efficient and consistent sample homogenization. It features variable speed control and a robust construction for reliable performance in various laboratory settings.
Lab products found in correlation
11 protocols using megamix
Platelet Activation by ADP and EcoHIV
Multiparametric Flow Cytometry Analysis
Fluorescent Bead-Based Assay Protocol
Multicolor Flow Cytometry for MP Enumeration
Isolation and Characterization of MSC-Derived Extracellular Vesicles
Fluorescent beads ranging in size from 0.1 to 1 μm (Megamix; BioCytex, Marseille, France) were employed to precisely gate EV. As EV derived from MSC express surface molecules that are characteristic of the cells of origin, anti‐rat CD49e (as positive marker) and anti‐rat CD45 (as negative marker) (both from BioLegend) were used. The analysis was performed by direct immunofluorescence with a Navios flow cytometer (Beckman Coulter), and the data were analysed using Kaluza software. Moreover, some specific exosomal markers, such as CD63, CD9 and CD81 (Miltenyi Biotec, Bergisch Gladbach, Germany), were also analysed, using the Guava easyCyte FlowCytometer (Millipore, Billerica, MA, USA) with InCyte software.
Quantification of Platelet-Derived Microparticles
Flow Cytometric Analysis of Immune Complexes
The sheath fluid for the cytometer was IsoFlow™ Sheath Fluid from Beckman Coulter, Part Number 8448010, an isotonic fluid at a pH 7.35-7.65, with Sodium Phosphate Dibasic, Sodium Fluoride, Diethylene Glycol Phenyl Ether, and 2-Phenoxyethanol.
A microparticle gate was established in agreement with the published method for characterization of circulating cell-derived microparticles by preliminary standardization experiments using a blend of size-calibrated 0.3, 0.5, 0.9, and 3 μm fluorescent beads, Megamix®, Biocytex, Marseille, France [27 (link), 28 (link)]. To enlarge the scale of fluorescent beads, in some experiments, Trucount beads (Beckton Dickinson) of 10 μm were added.
A gate for DNA-anti-DNA immune complexes for each series of experiments was built around DNA-anti-DNA immune complexes observed in the aliquot of the pool of 15 SLE serum samples incubated with calf thymus DNA. See below for more details and for compensations.
Flow Cytometric Analysis of Platelet Microparticles
To limit background noise from dust and crystals, flow cytometric analyses were performed using a 0.22 μm-filtered sheath fluid (IsoflowTM, Beckman Coulter). CXP ACQUISITION and CXP ANALYSIS software packages (Beckman Coulter) were used for data acquisition and analysis, respectively.
Extracellular Vesicle Antigen Analysis
Characterization of Microvesicle Composition
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