PPAR-γ (ab45036), interleukin (IL)-1β (ab9722), tumor necrosis factor-alpha (TNF-α; ab66579), and F4/80 (ab6640) antibodies were obtained from Abcam (Cambridge, UK). CD45 antibody (sc-52491) and rosiglitazone (sc-202795) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). IL-6 (12912S), NF-κB p65 (8242), phospho-NF-κB p65 (3033), stress-activated protein kinase (SAPK)/Jun-amino-terminal kinase (JNK; 9258), phospho-SAPK/JNK (9255S), p38 MAPK (9212L), phospho-p38 MAPK (4631L), p44/42 MAPK (Erk1/2) (4695S), and phospho-p44/42 MAPK (Erk1/2) (9101S) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Invitrogen Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A-11058); Invitrogen Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11055); and Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21206) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Horseradish peroxidase (HRP)-conjugated anti-β-actin antibody (a5316) was obtained from Sigma-Aldrich (St. Louis, MO, USA), and 4′,6-diamidino-2-phenylindole (DAPI; H-1200) and mounting medium (H-5000) were obtained from Vector Laboratories (Burlingame, CA, USA).
Tnf α ab66579
Manufactured by Abcam
Sourced in United Kingdom
TNF-α (ab66579) is a recombinant protein that represents the mature form of human tumor necrosis factor-alpha (TNF-α). TNF-α is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. This product is intended for use in biochemical and cellular assays.
Automatically generated - may contain errors
Lab products found in correlation
F4 80 ab6640, by Abcam (1 mentions)
Phospho p44 42 mapk erk1 2 9101s, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole dapi h 1200, by Vector Laboratories (1 mentions)
Il 6 ab6672, by Abcam (1 mentions)
Il 1β sc 7884, by Santa Cruz Biotechnology (1 mentions)
Oxldl, by Bio-Rad (1 mentions)
Hrp conjugated antibody, by Cell Signaling Technology (1 mentions)
2 protocols using tnf α ab66579
1
Molecular Signaling Pathway Analysis
2
Protein Expression Analysis in Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TNF-α (ab66579) and IL-6 (ab6672) primary antibodies were purchased from Abcam (Cambridge, UK). IL-1β (sc-7884) was obtained from Santa Cruz Biotechnology (Dallas, TX). VCAM-1 (12367s) and ICAM-1 (4915s) antibodies were purchased from Cell Signaling Technology (Beverly, MA). HRP-conjugated antibody (Cell Signaling Technology, Beverly, MA) was used as secondary antibody. Recombinant human sTREM-1 (1278-TR-050) and recombinant human TNF-α (210-TA-050) were obtained from R&D system (Minneapolis, MN). OxLDL was purchased from abD serotec (Oxford, UK).
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