Caki-1 cells, which were infected with control or miR-30c, were analyzed by trans-well assays performed in 24-well plates chamber (Corning Incorporated) fitted with a polyethylene terephthalate filter membrane with 8-µm pores. Next, the membrane’s undersurface was preincubated by 30 µL ECM (Extracellular matrix) from Sigma-Aldrich Co., mixed with serum-free RPMI 1640 in 1:5 dilution for 4 h at 37°C. The top chambers of the trans-wells were filled with 0.1 mL of cells (5×105 cells per mL) in serum-free medium, and the bottom chambers were filled with 0.3 mL of RPMI 1640 containing 10% FBS. The cells were incubated in the trans-wells at 37°C in 5% CO2 for 12 h. For migration assay, the cells were incubated in chambers without an ECM coating.
24 well plates chamber
Manufactured by Corning
Sourced in United States
The 24-well plates chamber is a laboratory equipment designed for cell culture applications. It provides a controlled environment for the cultivation and observation of cells. The 24-well format offers a compact and efficient setup for multiple simultaneous experiments or sample analyses.
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5 protocols using 24 well plates chamber
1
Trans-well migration assay for Caki-1 cells
2
Transwell Assay for Cell Migration and Invasion
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Caki-1 cells, which were infected with Ad-control or Ad-MEIS1, were analyzed by transwell migration assays performed in 24-well plates chamber (Corning, NY, USA) by fitted with a polyethylene terephthalate filter membrane with 8 μm pores. For invasion, the top chambers was coated with 30 μl ECM (Extracellular matrix) gel (Sigma, USA) diluted with serum free RPMI-1640 in 1:5 dilution for more than 4 h at 37 °C. Then, chambers were filled with 0.2 ml of cells (5 × 105 cells per ml) in serum-free medium, and the bottom chambers were filled with 0.25 ml of medium with 10% FBS. The cells were incubated in the trans-wells at 37 °C in 5% CO2 for 12 h. For migration, the cells were incubated in chambers without ECM coated. After 10-15 h (for invasion) or 4-6 h (for migration), chamber membrane was fixed with 4% paraformaldehyde and stained with crystal violet. The relative invading cells were measured according to the instructions provided [27 (link), 28 (link)]. Values were presented as the mean ± SD of triplicated experiments.
3
Transwell Assays for Tumor Cell Invasion
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MHCC97-H cells were injected into nude mice to produce subcutaneous tumors. Subsequently, the tumors were divided into an RFA-treatment group and a non-RFA treatment group. Next, single cells (3000 per well) were separated from the subcutaneous tumors and were analyzed by transwell assays performed in 24-well plates chamber (Corning, Lowell, MA, USA) fitted with a polyethylene terephthalate filter membrane with 8-μm pores. The invasion cells or migration cells were measured following the methods descripted by Zhou et al. and Yang et al. [61 , 62 (link)].
4
Transwell Assay for Invasion and Migration
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MHCC-97H cells, which were treated without or with a middle dose of 60Co-γ irradiation (6 Gy), were analyzed by transwell assays performed in 24-well plates chamber (Corning, Lowell, MA, USA) fitted with a polyethylene terephthalate filter membrane with 8-μm pores. The invasion-transwell or migration-transwell was performed following the methods descripted by Li et al., 2017 [62 (link)].
5
Transwell Assay for Cell Migration and Invasion
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Cells were infected with Ad-control or Ad-MEIS1, and then cells were analyzed by transwell migration assays performed in 24-well plates chamber (Corning, NY, USA) by fitted with a polyethylene terephthalate filter membrane with 8 μm pores. For invasion, the top chambers were coated with 30 μl ECM (Extracellular matrix) gel (Sigma, USA) diluted with serum free RPMI-1640 in 1:5 dilutions for more than 4 h at 37 °C. Then, chambers were filled with 0.2 ml of cells (5 × 105 cells per ml) in serum-free medium, and the bottom chambers were filled with 0.25 ml of medium with 10% FBS. The cells were incubated in the trans-wells at 37 °C in 5% CO2 for 12 h. For migration, the cells were incubated in chambers without ECM coated. After 10-15 h (for invasion) or 4-6 h (for migration), chamber membrane was fixed with 4% paraformaldehyde and stained with crystal violet. The relative invading cells were measured according to the instructions provided [27 (link), 28 (link)]. Values were presented as the mean ± SD of triplicated experiments.
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