8 cyclopentyl 13 dipropyl xanthine dpcpx

Chemicals were purchased from Gibco (USA)
or Sigma-Aldrich (USA) unless indicated
otherwise. Materials purchased from Sigma-
Aldrich included platelet derived growth
factor-AA (PDGF-AA), epidermal growth
factor (EGF), triiodothyronine (T3), Matrigel,
all-trans retinoic acid (RA), paraformaldehyde,
Triton, 4’,6-diamidino-2-phenylindole (DAPI),
5-bromo-2´-deoxyuridine (BrdU), and N6-
cyclopentyladenosine (CPA). Materials
purchased from Gibco included DMEM/
F12, insulin-transferrin-selenium (ITS),
N2 supplement, fetal bovine serum (FBS),
penicillin/streptomycin, L-glutamine, nonessential
amino acids (NEAA), and B27
supplement. We purchased 5´-Chloro-5´-
deoxy-endo-2-norbornyl adenosine (5´Cl5´d-
(±)-ENBA) from Tocris and 8-Cyclopentyl-1
3-dipropyl xanthine (DPCPX) from Abcam.

The aCSF used for dissections and recordings contained (in mM) 127 NaCl, 26 NaHCO₃, 10 glucose, 3 KCl, 2 CaCl, 1.25 NaH₂PO₄, and 1 MgCl₂. Adenosine, 4-hydroxytamoxifen, and TFLLR were supplied by Sigma-Aldrich (Poole, UK); 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) was supplied by Abcam (Cambridge, UK); 14–22 amide, SKF 38393, and tetrodotoxin (TTX) were supplied by Tocris Bioscience (Bristol, UK). TFLLR, 14–22 amide, and SKF 38393 were dissolved in reverse-osmosis water; 4-hydroxytamoxifen was dissolved in corn oil; TTX was dissolved in citrate buffer; and Adenosine and DPCPX were dissolved in DMSO. The concentration of DMSO in working solutions did not exceed 0.1% (vol/vol).