Bond refine polymer

Manufactured by Leica

The Bond Refine PolymerTM is a laboratory equipment product by Leica. It is designed for the preparation and processing of samples for various analytical techniques. The core function of this product is to facilitate the effective extraction, purification, and concentration of target analytes from complex matrices.

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Lab products found in correlation

Bond 3, by Leica (2 mentions) De r 11, by Roche (1 mentions) G219 1129, by Cell Marque (1 mentions) Mrq 28, by Cell Marque (1 mentions)

2 protocols using bond refine polymer

Immunohistochemical Evaluation of Muscle and Mismatch Repair Proteins

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Immunohistochemical staining was performed using an automated immunostainer (Leica Bond‐III, Leica Biosystems, Buffalo Grove, IL) and Bond Refine PolymerTM biotin‐free DAB detection kit. Formalin‐fixed, paraffin‐embedded tissue sections were stained with desmin (mouse monoclonal, DE‐R‐11, predilute ready‐to‐use, Ventana, Oro Valley, AZ), h‐caldesmon (mouse monoclonal, h‐CALD, 1:400 dilution, Thermo Fisher, Waltham, MA), smooth muscle actin (mouse monoclonal, 1A4, predilute ready‐to‐use, Rocklin, CA), MyoD1 (rabbit monoclonal, Ep212, predilute ready‐to‐use, Rocklin, CA), myogenin (mouse monoclonal, FSD, predilute ready‐to‐use, Ventana, Oro Valley, AZ), MLH1 (mouse monoclonal, ES05, Leica Biosystems, Buffalo Grove, IL), MSH2 (mouse monoclonal, 79H11, Leica Biosystems, Buffalo Grove, IL), MSH6 (rabbit monoclonal, EP49, Leica Biosystems, Buffalo Grove, IL), PMS2 (mouse monoclonal, EP51, Leica Biosystems, Buffalo Grove, IL). A positive nuclear, cytoplasmic and/or membranous expression was identified in 10% or more of neoplastic cells qualified as “positive (+).”

Sukhanova M., Obeidin F., Streich L, & Alexiev B.A. (2022). Inflammatory leiomyosarcoma/rhabdomyoblastic tumor: A report of two cases with novel genetic findings. Genes, Chromosomes & Cancer, 61(11), 653-661.

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Immunohistochemical Evaluation of MMR and MSI

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All tumors were evaluated for mismatch repair (MMR) deficiency and microsatellite instability (MSI) via immunohistochemical approach in a Clinical Laboratory Improvement Amendments-approved laboratory for clinical care. Immunohistochemical staining was performed using an automated immunostainer (Leica Bond-III; Leica Biosystems, Buffalo Grove, IL) and Bond Refine PolymerTM biotin-free 3,3’-diaminobenzidine (DAB) detection kit. Antibodies included MLH1 (mouse anti-human antibody clone ES05, Leica, Buffalo Grove, IL), MSH2 (mouse anti-human antibody clone G219–1129, Cell-Marque, Rocklin, CA), MSH6 (mouse anti-human clone 44, Cell-Marque, Rocklin, CA) and PMS2 (mouse anti-human antibody clone MRQ-28, Cell-Marque, Rocklin, CA). Bright signal intensity in greater than 1% of tumor cells was considered positive for protein expression[12 (link), 13 (link)].

Escobar D., Jones R., Gao J., Sun L., Liao J, & Yang G.Y. (2020). Unique clinicopathologic and genetic alteration features in early onset colorectal carcinoma (CRC) compared to age-related CRC: large cohort next generation sequence analysis. Human pathology, 105, 37-46.

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