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T ferulic acid

Manufactured by Merck Group
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Sourced in United States, Italy

T-ferulic acid is a chemical compound that serves as a key ingredient in certain pharmaceutical and laboratory applications. It is a natural phenolic compound derived from the ferulic acid molecule. The core function of T-ferulic acid is to act as a versatile chemical building block for various research and development purposes.

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Lab products found in correlation

P coumaric acid, by Merck Group (9 mentions) Gallic acid, by Merck Group (8 mentions) Caffeic acid, by Merck Group (7 mentions) Chlorogenic acid, by Merck Group (7 mentions) Syringic acid, by Merck Group (7 mentions) Vanillic acid, by Merck Group (5 mentions) Sinapic acid, by Merck Group (5 mentions) Rutin, by Merck Group (5 mentions) Catechin, by Merck Group (4 mentions) Protocatechuic acid, by Merck Group (4 mentions) Epicatechin, by Merck Group (4 mentions) P hydroxybenzoic acid, by Merck Group (4 mentions) Kaempferol, by Merck Group (4 mentions) T cinnamic acid, by Merck Group (4 mentions) Quercetin, by Merck Group (3 mentions) Salicylic acid, by Merck Group (3 mentions) Milli q system, by Merck Group (2 mentions) Acetonitrile, by Carlo Erba (2 mentions) Ethanol, by Carlo Erba (2 mentions) Acetic acid, by Carlo Erba (2 mentions)

Spelling variants of this product

Ferulic acid (788 citations) Ferulic (62 citations)

10 protocols using t ferulic acid

1

Phenolic Profile Analysis of Food Samples

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The following chemicals were purchased from Carlo Erba Reagents (Milan, Italy): acetic acid, acetonitrile, citric acid, ethanol, Folin–Ciocalteu’s Reagent (FCR), n-hexane, potassium chloride, sodium acetate, sodium carbonate, and trichloroacetic acid.
Phenolic compound standards (i.e., gallic acid, protocatechuic acid, catechin, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, epicatechin, caffeic acid, syringic acid, p-coumaric acid, t-ferulic acid, sinapic acid, rutin, cyanidin-3-O-glucoside, peonidini-3-O-glucoside, and malvidin) were purchased from Sigma-Aldrich (Milan, Italy) and Extrasynthèse (Geney, France). The aqueous solutions used in HPLC analysis were prepared with water that had been purified using a Milli-Q system (Millipore Corp., Billerica, MA, USA). All reagents and solvents were HPLC- or analytical-grade.
Melini V., Melini F., Luziatelli F, & Ruzzi M. (2023). Development of an Ultrasound-Assisted Extraction Procedure for the Simultaneous Determination of Anthocyanins and Phenolic Acids in Black Beans. Foods, 12(19), 3566.
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2

Antioxidant and Phenolic Profiling

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2-2′-Azino-bis(3-ethylbeenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), ethanol (CHROMASOLV®, 99.8%), ethylacetate (CHROMASOLV®, 99.8%), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, 97%), hydrochloric acid (HCl, 37.0%), Iron (III) chloride hexahydrate (FeCl3*6H2O, ≥ 98.0%), methanol (CHROMASOLV®, 99.9%), potassium sulfate (K2SO4, ≥ 99.0%), sodium hydroxide (NaOH, ≥ 98.0%), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), trifluoroacetic acid (TFA), sodium carbonate (Na2CO3) and phenolic acid standards (caffeic acid ≥ 98%, p-coumaric acid ≥ 98%, t-ferulic acid ≥ 99%, gallic acid ≥ 99%, protocatechuic acid ≥ 99%, p-hydroxybenzoic acid ≥ 99%, sinapic acid ≥ 98%, syringic acid ≥ 95% and vanillic acid ≥ 97%) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Monosaccharide standards (L(+) Arabinose and D(+) Xylose) were purchased from Extrasynthese (Genay, France). Water (HPLC grade) was obtained from ELGA PURELAB Ultra system (M-medical, Cornaredo, Milan, Italy).
Sardella C., Capo L., Adamo M., Donna M., Ravetto Enri S., Vanara F., Lonati M., Mucciarelli M, & Blandino M. (2023). The cultivation of rye in marginal Alpine environments: a comparison of the agronomic, technological, health and sanitary traits of local landraces and commercial cultivars. Frontiers in Plant Science, 14, 1130543.
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3

HPLC Analysis of Phenolic Compounds

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The standards used (p‐coumaric acid, 3,4 dihydroxybenzoic acid, quercetin, caffeic acid, t‐ferulic acid, chlorogenic acid, gallic acid, epicathechin, pyracathechol, sinapic acid, vanilic acid, syringic acid, and phlorizin) and Folin–Ciocalteau reagent and acetonitrile were from Sigma–Aldrich (St. Louis, MO, USA). Pesticide standards were obtained as a mixture of reference components (CPAchem Ltd, Bogomilovo, Bulgaria). All reagents and standards used were HPLC grade, and purified water from a Milli Q system (Merck KGaA, Darmstadt, Germany) was used throughout the experiments.
Evran E., Durakli‐Velioglu S., Velioglu H.M, & Boyaci I.H. (2023). Effect of wax separation on macro‐ and micro‐elements, phenolic compounds, pesticide residues, and toxic elements in propolis. Food Science & Nutrition, 12(3), 1736-1748.
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4

Analytical Standards for Antioxidant Assays

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2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS.+), 2,2 diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), analytical standards of phenolic acids viz., gallic acid; GA, 2,5-dihydroxybenzoic acid; 2,5-DHA, p-hydroxybenzoic acid; p-HA, vanillic acid; VA, syringic acid; SYA, p- coumaric acid; p-CA, chlorogenic acid; CHL, t-cinnamic acid; t-ferulic acid; t-FA, Sinapic acid; SIA, and the flavonoids kaempferol; KAM, (±) and Catechin hydrate CH were purchased from Sigma Aldrich, Chemical Co. (St. Louis, MO, USA). HPLC grade methanol, acetic acid, and acetonitrile used for extraction and HPLC analysis were purchased from Merck, India Pvt. Ltd. All other chemicals used were of analytical grade or above.
Avinash G., Sharma N., Prasad K.R., Kaur R., Singh G., Pagidipala N, & Thulasinathan T. (2024). Unveiling the distribution of free and bound phenolic acids, flavonoids, anthocyanins, and proanthocyanidins in pigmented and non-pigmented rice genotypes. Frontiers in Plant Science, 15, 1324825.
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5

Phenolic Compound Chemical Standards

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Chemical standards of phenolic compounds (benzoic acid, carvacrol, catechin, chlorogenic acid, t-cinnamic acid, 8-cinnamoyl harpagide (harpagoside), o-coumaric acid, p-coumaric acid, 2,3-dimethoxybenzoic acid, epicatechin, t-ferulic acid, gallic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 3-hydroxy-4-methoxybenzaldehyde, naringin, naringenin, quercetin, rutin, sinapinic acid, syringic acid, vanillic acid (all purity > 98%)), β-cyclodextrin (≥97%), n-hexane (HPLC-grade), diethyl ether (≥99%), and chloroform (HPLC-grade) were purchased from Sigma-Aldrich (Milan, Italy).
Ethyl acetate (≥99%), acetonitrile (HPLC-grade), methanol (HPLC-grade), ethanol (HPLC-grade), acetic acid (≥99%) as well as d-(+)-glucose were obtained from Carlo Erba Reagents (Milan, Italy). Sodium chloride (≥99%) was obtained from Honeywell (Seelze, Germany). NADES (glycolic acid/betaine mixture) was newly synthesized and supplied by University of Perugia. It was chosen between differently structured novel DES and NADES mixtures for its suitable properties (low freezing point and low viscosity, absence of aromatic compounds in its composition, low cost and natural source of the molecules forming it). Ultra-pure water was obtained using a Millipore Milli-Q Plus water treatment system (18 MΩ cm at 23 °C, Millipore Bedford Corp., Bedford, MA, USA).
Mocan A., Diuzheva A., Bădărău S., Moldovan C., Andruch V., Carradori S., Campestre C., Tartaglia A., De Simone M., Vodnar D., Tiecco M., Germani R., Crișan G, & Locatelli M. (2019). Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays. Molecules, 24(7), 1226.
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6

Anthraquinones and Phenolics Analysis

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Anthraquinones chemical standards (emodine, rhein, chrisophanol, aloe-emodine, and physcione; all purity ≥99%) were purchased from Extrasynthese (Genay, France). Phenolics as chemical standards (gallic acid, catechin, chlorogenic acid, p-hydroxy-benzoic acid, vanillic acid, epicatechin, syringic acid, 3-hydroxy-benzoic acid, 3-hydroxy-4-methoxybenzaldehyde, p-coumaric acid, rutin, sinapinic acid, t-ferulic acid, naringin, 2,3-dimethoxy benzoic acid, benzoic acid, o-coumaric acid, quercetin dihydrate, t-cinnamic acid, naringenin (all purity >98%)) were purchased from Sigma Aldrich (Milan, Italy). Methanol (HPLC-grade) and formic acid (99%) were obtained from Carlo Erba Reagenti (Milan, Italy). Double-distilled water was obtained using a Millipore Milli-Q Plus water treatment system (Millipore Bedford Corp., Bedford, MA).
Locatelli M., Zengin G., Uysal A., Carradori S., De Luca E., Bellagamba G., Aktumsek A, & Lazarova I. (2016). Multicomponent pattern and biological activities of seven Asphodeline taxa: potential sources of natural-functional ingredients for bioactive formulations. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 60-67.
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7

Antioxidant Standards Characterization

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BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene), DPPH (1,1-diphenyl-2-picryl-hydrazyl), neocuproine (2,9-dimethyl-1,10-phenanthroline), α-tocopherol, and trolox were obtained from Sigma (Sigma-Aldrich GmbH, Sternheim, Germany). The standard phenolic compounds of LC-MS/MS; Apigenin (95%), caffeic acid (98%), chlorogenic acid (95%), fumaric acid (99%), kaempferol (96%), pyrogallol (98%), rosmarinic acid (96%), rutin (94%), syringic acid (95%), t-ferulic acid (99%), quercitrin (97%), salicylic acid (99%), pcoumaric acid (98%), and pyrogallol (98%) were obtained from Sigma-Aldrich. Rhamnetin (98%) from ExtraSynthese, gallic acid (99%,) from Merck, and luteolin (99%) from AppliChem were used. Methanol obtained from Merck and all other reagents were purchased from Sigma-Aldrich or Fluka.
Bursal E., Aras A., Kılıç Ö, & Buldurun K. (2021). Chemical constituent and radical scavenging antioxidant activity of Anthemis kotschyana Boiss. Natural product research, 35(22).
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8

Quantification of Phenolic Compounds

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Folin-Ciocalteu's phenol reagent, sodium carbonate (99%), (+)-catechin (98%), sodium nitrite (97%), aluminium chloride (99.9%), and vanillin (99%) were purchased from Sigma-Aldrich (St. Louis, USA). Sulphuric acid (95-97%) was purchased from Fluka (Seelze, Germany). Sodium hydroxide (98%) was purchased from Merck (Darmstadt, Germany). Methanol (99.9%) was purchased from Carlo Erba Reagents (Rodano, Italy). Acetonitrile for LC-MS Ultra Chromasolv was purchased from Honeywell Riedel-de Haën TM (Seelze, Germany). Milli-Q® water (18.2 MΩ.cm) was obtained in a Millipore -Direct Q3 UV System equipment (Molsheim, France). Formic acid (98%) was obtained from Carl Roth (Karlsruhe, Germany). Eluents A and B used for Q-Orbitrap were from Optima™ LC/MS Grade, Fisher Scientific (NH, USA). Gallic acid, protocatechuic acid, p-hydroxybenzoic acid, sinapic acid, catechin, epicatechin, caffeic acid, p-coumaric acid, t-ferulic acid, kaempferol, quercetin were obtained from Sigma-Aldrich Co. (Steinheim, Germany).
Mecha E., Erny G.L., Guerreiro A.C.L., Feliciano R.P., Barbosa I., Bento da Silva A., Leitão S.T., Veloso M.M., Rubiales D., Rodriguez-Mateos A., Figueira M.E., Vaz Patto M.C, & Bronze M.R. (2022). Metabolomics profile responses to changing environments in a common bean (Phaseolus vulgaris L.) germplasm collection. Food chemistry, 370.
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9

Quantification of Polyphenol Standards in LC-MS/MS

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The following compounds were used as standards in LC-MS/MS analyses: fumaric acid (99%, Sigma-Aldrich), pyrogallol (98%, Sigma-Aldrich), rutin (94%, Sigma-Aldrich), chlorogenic acid (95%, Sigma-Aldrich), gallic acid (99%, Merck), syringic acid (95%, Sigma-Aldrich), t-ferulic acid (99%, Sigma-Aldrich), caffeic acid (98%, Sigma-Aldrich), pelargonin chloride (98%, Sigma-Aldrich), quercitrin (97%, Sigma-Aldrich), salicylic acid (99%, Sigma-Aldrich), p-coumaric acid (98%, Sigma-Aldrich), luteolin-7-O-glu (99%, AppliChem), rosmarinic acid (96%, Sigma-Aldrich), pyrogallol (98%, Sigma-Aldrich), apigenin (95%, Sigma-Aldrich), kaempferol (96%, Sigma-Aldrich), and isorhamnetin (98%, ExtraSynthese, Genay-France).
Çarıkçı S. (2020). Antioxidant and Anticholinesterase Properties of Sideritis perfoliata subsp. athoa (Papan. &Kokkini) Baden and Sideritis trojana Bornm. Teas from Mount Ida-Turkey and Their Phenolic Characterization by LC-MS/MS. Journal of the Turkish Chemical Society Section A Chemistry, 7(2), 617-634.
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10

Phenolic and Carbohydrate Standard Preparation

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Phenolic acid standards (3-(4-hydroxyphenyl) propionic acid, 4-hydroxy benzoic acid, caffeic acid, chlorogenic acid, p-coumaric acid, protocatechuic acid, salicylic acid, sinapic acid, t-cinnamic acid, t-ferulic acid, and vanillic acid) and carbohydrate standards (fructose, glucose, sucrose, and sorbitol) were purchased from Sigma-Aldrich (MO, USA). Acetic acid (85:15) and HPLC-grade methanol were obtained from Fisher Scientific (NJ, USA). Butylated hydroxyanisole (BHA) and LC/MS grade acetonitrile were obtained from Acros Organic (NJ, USA) and Burdick and Jackson (MI, USA), respectively.
Lee J., Chan B.L, & Mitchell A.E. (2017). Identification/quantification of free and bound phenolic acids in peel and pulp of apples (Malus domestica) using high resolution mass spectrometry (HRMS). Food chemistry, 215.
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