Epx precision microplate reader

To determine the cytotoxicity of cells after exposure to AMB or ND-AMB (18 h), culture medium was tested for the presence of released lactate dehydrogenase (LDH). A 96-well LDH Cytotoxicity Assay Kit (Cayman Chemical Company, Ann Arbor, MI) was used, with the following modifications: on the day of the assay, 100 μL of culture medium was removed and added to 100 μL of Reaction Solution. Absorbance at 490 nm was obtained with Epx Precision Microplate Reader (Molecular Devices, Sunnyvale, CA). We measured the LDH levels 30 min after exposure. Triton X-100 (10%) was used as a positive control (maximum release of LDH) and cell medium (without phenol) was used as a negative control (spontaneous release of LDH). % Cytotoxicity of test sample was calculated based on below formula: $\%\text{Cytotoxicity}=\frac{\left(\text{Experimental}\text{value}\right)-\left(\text{Spontaneous}\text{release}\right)}{\left(\text{Maximum}\text{release}\right)-\left(\text{Spontaneous}\text{release}\right)}\times 100$