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2 protocols using concentrated ammonia aqueous solution

1

Synthesis and Characterization of Multifunctional Silica Nanoparticles

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Anhydrous ethanol, concentrated ammonia aqueous solution (NH3•H2O, 25 wt%), tetraethyl orthosilicate (TEOS), Bis[3-(triethoxysilyl) propyl] tetrasulfide (TETS), Dimethyl sulfoxide (DMSO), cetyltrimethylammonium bromide (CTAB), concentrated hydrochloric acid (HCl, 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). 3-aminopropyltriethoxysilane (APTES) was purchased from Sigma-Aldrich (USA). Cell culture medium (Dulbecco’s Modified Eagle’s Medium, DMEM), Dimethyl sulfoxide (DMSO), penicillin, fetal bovine serum (FBS) and Trypsin were provided by Gibco (USA). Bovine serum albumin (BSA) was provided by Promag (USA). 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) and lactoferrin (Lf) was purchased from Nanjing Kengen (China). Doxorubicin hydrochloride (Dox) purchased from Sangon (China). All water was deionized water (Millipore, USA) with a resistivity of 18.2 MΩ/cm. Human embryonic kidney cells 293T, rat glioma cells C6 were purchased from American Type Culture Collection (ATCC) Cell bank.
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2

Synthesis and Characterization of Multifunctional Silica Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anhydrous ethanol, concentrated ammonia aqueous solution (NH3•H2O, 25 wt%), tetraethyl orthosilicate (TEOS), Bis[3-(triethoxysilyl) propyl] tetrasulfide (TETS), Dimethyl sulfoxide (DMSO), cetyltrimethylammonium bromide (CTAB), concentrated hydrochloric acid (HCl, 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). 3-aminopropyltriethoxysilane (APTES) was purchased from Sigma-Aldrich (USA). Cell culture medium (Dulbecco’s Modified Eagle’s Medium, DMEM), Dimethyl sulfoxide (DMSO), penicillin, fetal bovine serum (FBS) and Trypsin were provided by Gibco (USA). Bovine serum albumin (BSA) was provided by Promag (USA). 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) and lactoferrin (Lf) was purchased from Nanjing Kengen (China). Doxorubicin hydrochloride (Dox) purchased from Sangon (China). All water was deionized water (Millipore, USA) with a resistivity of 18.2 MΩ/cm. Human embryonic kidney cells 293T, rat glioma cells C6 were purchased from American Type Culture Collection (ATCC) Cell bank.
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