B6 cg tyrc 2j j

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro

B6(Cg)-Tyrc-2J/J is a mouse strain available from Jackson ImmunoResearch. It is a congenic strain, meaning it has been bred to have a specific genetic background. The 'Tyrc-2J/J' designation indicates that this strain has a mutation in the tyrosinase gene, which results in albinism.

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19 protocols using b6 cg tyrc 2j j

Mouse Embryonic Stem Cell Lines for Imaging

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Mouse embryonic stem cell lines for imaging the Pou5f1 and Nanog loci were derived from Bruce 4 mESCs (Millipore Sigma CMTI-2; murine strain C57/BL6J, male – species/sex verified by karyotyping, no additional cell line authentification performed). OMG mESCs^{39 (link)} contain a 24 × MS2 cassette integrated in the 3′-UTR of one of the two Pou5f1 alleles^{57 (link)} and also stably express MCP-mNeonGreen^{39 (link)}. NMG mESCs contain 24 × MS2 cassettes integrated in the 3′-UTR of both Nanog alleles and also stably express MCP-mNeonGreen^{58 (link)}. NMG SNAP-Brd4 clone 7 cells also contain bi-allelic integrations of SNAP-tag at the Brd4 locus^{39 (link)}. mESC lines for imaging the Sox2 locus were derived from B6 albino mESCs (B6(Cg)-Tyrc-2J/J, Jackson Lab 000058; male - species/sex verified by karyotyping, no additional cell line authentification performed). All cell lines tested negative for mycoplasma.

Li J., Hsu A., Hua Y., Wang G., Cheng L., Ochiai H., Yamamoto T, & Pertsinidis A. (2020). Single-gene imaging links genome topology, promoter-enhancer communication and transcription control. Nature structural & molecular biology, 27(11), 1032-1040.

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Multimodal Immune Cell Profiling

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C57BL/6J, B6.129(Cg)-Ccr2^tm2.1Ifc/J (CCR2-RFP), B6.Cg-Tg(Itgax-Venus)1Mnz/J (CD11c-YFP), B6(Cg)-Tyr^c−2J/J (B6 albino), B6.Cg-Zbtb46^{tm4.1(HBEGF)MnzTyrc−2J}/J (Zbtb46-DTR), B6.129P2(C)-Ccr7^tm1Rfor/J (CCR7.KO), B6.129S1-Il12^btm1Jm/J (IL-12p40.KO), and B6.129S4-Ccr2^tm1Ifc/J (CCR2.KO) mouse strains were obtained from The Jackson Laboratory. CD45.1⁺ B6.Cg-Tg(TcraTcrb)425Cbn/J (OT-II), CD45.1⁺ C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I), and B6.SJL-Ptprc^aPepc^b/BoyCrl (CD45.1⁺) were obtained either from donating investigators (Dr. Pamela J. Fink, University of Washington) or Charles River. CD11c-YFP animals were crossed with B6 albino mice to homozygosity, and next crossed to CCR2-RFP mice to generate a CD11c-YFP x CCR2-RFP^Hetrozygous dual reporter mice. CCR2-DTR mice were obtained from donating investigators (Dr. Steven F. Ziegler, Benaroya Research Institute) and with approval from the originating investigators (Drs. Tobias M. Hohl and Eric G. Pamer, Memorial Sloan-Kettering Cancer Center) (95 (link)). 6–10 week-old male and female mice were kept in specific pathogen–free conditions at an Association for Assessment and Accreditation of Laboratory Animal Care–accredited animal facility at the University of Washington, South Lake Union campus. All procedures were approved by the University of Washington Institutional Animal Care and Use Committee.

Leal J.M., Huang J.Y., Kohli K., Stoltzfus C., Lyons-Cohen M.R., Olin B.E., Gale M J.r, & Gerner M.Y. (2021). Innate cell microenvironments in lymph nodes shape the generation of T cell responses during Type-I inflammation. Science immunology, 6(56), eabb9435.

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Glucose Homeostasis during Estrus Cycle in Mice

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The glucose homeostasis varies throughout the estrus cycle in female mice (42 (link)). To avoid the variation caused by estrus cycles, only male mice were used in this study. All mice were maintained on a C57BL/6J-albino background (Jackson Laboratory; B6(Cg)-Tyrc-2J/J). Littermates of Prkar1a^fl/fl mice (Jackson Laboratory; Prkaa1^tm1.1Sjm/J) were placed on a normal chow for 11 weeks after weaning and then injected with either Ad5-CMV-CRE or Ad5-CMV-GFP (3 × 10⁹ pfu/mouse; University of Iowa Viral Vector Core) via the tail vein, resulting in L-GFP and L-PKA mice. All experiments were performed between 2 and 5 weeks after the injection. All mice described were housed in a pathogen-free barrier facility with a 12-h light/dark cycle. All animal protocols were approved by the Institutional Animal Care and Use Committee of Rutgers University.

Xu H., Wang Y., Kwon H., Shah A., Kalemba K., Su X., He L, & Wondisford F.E. (2022). Glucagon changes substrate preference in gluconeogenesis. The Journal of Biological Chemistry, 298(12), 102708.

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Dlk1-FLucLacZ Mice Husbandry

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Mice were handled and all in vivo studies were performed in accordance with the United Kingdom Animals (Scientific Procedures) Act (1986). Mouse work was approved by the Imperial College AWERB committee and performed under a UK Home Office project license. Mice were housed on a 12-h light-dark cycle with a temperature range of 21 + /− 2 °C and a humidity range of 55 + /− 10% in pathogen-free conditions. The Dlk1-FLucLacZ line was maintained on a B6(Cg)-Tyr^c-2J/J (Jackson Labs, C57Bl/6 J albino) background. For mating, males were set up with not more than three females and morning plug checking was performed. Upon plug discovery, females were considered E0.5.

Van de Pette M., Dimond A., Galvão A.M., Millership S.J., To W., Prodani C., McNamara G., Bruno L., Sardini A., Webster Z., McGinty J., French P.M., Uren A.G., Castillo-Fernandez J., Watkinson W., Ferguson-Smith A.C., Merkenschlager M., John R.M., Kelsey G, & Fisher A.G. (2022). Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice. Nature Communications, 13, 2464.

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Adoptive T Cell Transfer in IL-10 Reporter Mice

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Specific pathogen-free C57BL/6, B6(Cg)-Tyrc-2J/J, and IL-10gfp reporter transgenic mice on a C57BL/6 background were acquired from The Jackson Laboratory (B6.129S6-Il10^tm1Flv/J stock # 8379, “TIGER”, and B6(Cg)-Il10^tm1.1Karp/J stock #14530, “Vert-X) and bred in house with B6(Cg)-Tyrc-2J/J (albino B6) for heterozygosity and homozygosity. 6–12-wk-old adult mice were used in all experiments. B6(Cg)-Tyrc-2J/J (albino B6) were also used where indicated. All mice were housed under specific pathogen–free conditions (including MNV, MPV, and MHV) and maintained on standard rodent chow and water supplied ad libitum.
For adoptive T cell transfer, spleens and nodes were removed from OT-I dsRed mice (as described in [26 (link), 60 (link)]). Cells were purified to > 90% purity using an Automacs (Miltenyi Biotech) and using negative selection for CD8⁺ T cells. At least one day prior to infection, 1 x 10⁵ cells were transferred into IL-10gfp animals.

Cush S.S., Reynoso G.V., Kamenyeva O., Bennink J.R., Yewdell J.W, & Hickman H.D. (2016). Locally Produced IL-10 Limits Cutaneous Vaccinia Virus Spread. PLoS Pathogens, 12(3), e1005493.

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Murine Models for Ophthalmic Research

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All mouse procedures were performed in accordance with the Association for Vision and Ophthalmology’s guidelines for the use of animals in ophthalmic research. B6, A/J and B6(Cg)-Tyr^c-2J/J (B6 albino) mice were obtained from the Jackson Laboratory (Bar Harbor, ME). Consomic lines C57BL/6J-Chr7^A/NaJ (Con7) and C57BL/6J-Chr19^A/NaJ (Con19) were generated as described by Nadeau et al. (2000) (link) and obtained from the Jackson Laboratory. Recombinant inbred strains (A×B and B×A recombinant inbred panels) used for QTL mapping were also obtained from the Jackson Laboratory (Bailey 1971 (link), Taylor 1978 ).

Higuchi H., Macke E.L., Lee W.H., Miller S.A., Xu J.C., Ikeda S, & Ikeda A. (2014). Genetic basis of age-dependent synaptic abnormalities in the retina. Mammalian genome : official journal of the International Mammalian Genome Society, 26(0), 21-32.

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Murine Model of Melioidosis under Biosafety Level 3

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All animal studies were conducted under Biosafety Level 3 conditions using 8–10 week old female albino C57BL/6J mice (B6 (Cg)-Tyr^c−2J/J, Jackson Laboratories) bred at the University of Louisville (Protocol Number 11113). These studies were approved by the University of Louisville Institutional Animal Care and Use Committee (Protocol numbers 10073 and 13053) in agreement with NIH guidelines and the “Guide for the Care and Use of Laboratory Animals” (NRC). University of Louisville is approved for use of the Tier 1 select agent B. pseudomallei with continuous registration from the Centers for Disease Control since 2010.

Gutierrez M.G., Yoder-Himes D.R, & Warawa J.M. (2015). Comprehensive identification of virulence factors required for respiratory melioidosis using Tn-seq mutagenesis. Frontiers in Cellular and Infection Microbiology, 5, 78.

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Ophthalmic Research in Rodents

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All experimental protocols were approved by the Johns Hopkins Animal Care and Use Committee. All animals were handled and treated in accordance with the Association for Research in Vision and Ophthalmology (ARVO) Statement for Use of Animals in Ophthalmic and Vision Research. A specific sex was not specified when ordering any species, so both male and female animals were used as provided. C57BL/6J and tyrosinase knockout mice on the C57BL/6J background (B6(Cg)-Tyr^c−2J/J) were purchased from The Jackson Laboratory. Brown Norway and Wistar rats were obtained from Charles River Laboratories. All animals were 6–8 weeks in age. Animals were anesthetized prior to euthanasia.

Kim Y.C., Hsueh H.T., Shin M.D., Berlinicke C.A., Han H., Anders N.M., Hemingway A., Leo K.T., Chou R.T., Kwon H., Appell M.B., Rai U., Kolodziejski P., Eberhart C., Pitha I., Zack D.J., Hanes J, & Ensign L.M. (2021). A hypotonic gel-forming eye drop provides enhanced intraocular delivery of a kinase inhibitor with melanin-binding properties for sustained protection of retinal ganglion cells. Drug delivery and translational research, 12(4), 826-837.

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Albino Mouse Husbandry Protocol

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All animal protocols were approved by the Institutional Animal Care and Use Committee of Rutgers University. B6(Cg)-Tyrc-2j/J or B6 albino mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Mice were fed ad libitum standard chow and kept at 12 h light/12 h dark cycles.

Kalemba K.M., Wang Y., Xu H., Chiles E., McMillin S.M., Kwon H., Su X, & Wondisford F.E. (2019). Glycerol induces G6pc in primary mouse hepatocytes and is the preferred substrate for gluconeogenesis both in vitro and in vivo. The Journal of Biological Chemistry, 294(48), 18017-18028.

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Murine Strain Standardization for Animal Research

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All experiments were performed in accordance with the Institutional Animal Care and Use Committee of New York University Health. Six- to twelve-week-old female C57BL/6 albino mice, B6(Cg)-Tyr^c−2J/J, were purchased from Jackson Laboratory (stock no.: 000058).

Opp S., Hurtado A., Pampeno C., Lin Z, & Meruelo D. (2022). Potent and Targeted Sindbis Virus Platform for Immunotherapy of Ovarian Cancer. Cells, 12(1), 77.

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