Sections were deparaffined and hydrated before antigen retrieval in 10 mM citric acid buffer. Then, the sections were incubated in 1% Triton X-100 for 15 min, and 3% hydrogen peroxide was used for 15 min to remove endogenous peroxidase activity. The sections were washed with PBS three times and incubated with primary anti-SHMT2 antibody (Abcam Inc.) at 4°C overnight. Then, the sections were washed with PBS and incubated with specific secondary antibodies labeled with tetramethylrhodamine (red) and DAPI (blue) for 30 min at room temperature. The sections were then washed with PBS and sealed with Fluor Fluoromount-G™ slide mounting medium (Southern Biotech, Birmingham, AL, USA). Images were taken using a Zeiss LSM 510 confocal microscope (magnification: 200; ZeissAG, USA).
Fluor fluoromount g slide mounting medium
Manufactured by Southern Biotech
Sourced in United States
Fluor Fluoromount-G™ is a slide mounting medium formulated for use in fluorescence microscopy. It is designed to preserve the fluorescence of labeled samples and provide a durable, transparent seal for mounted slides.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using fluor fluoromount g slide mounting medium
1
Immunostaining of SHMT2 in Tissue Sections
2
TIM-4 Protein Immunofluorescence Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
KCs derived from recipient (sham, control mAb or TIM-4 mAb mice) were collected and were fixed in 4% paraformaldehyde for 1 h at room temperature. Slides were washed with PBS three times (3 min/wash) and blocked with 30 min in 10% FCS at room temperature. Slides were then washed with PBS three times (3 min/wash) and incubated with TIM-4 antibodies (RMT4-53, cat. no. GTX14149; 1:100 dilution; GeneTex Inc.) overnight at 4°C. Slides were washed with PBS three times (3 min/wash) and incubated with specific secondary antibodies labeled with tetramethylrhodamine (red; 1:100 dilution; cat. no. GMS40036; Bio Valley, Shanghai; China) or DAPI (blue) for 30 min at room temperature. Slides were then washed with PBS three times (3 min/wash) and sealed with FluorFluoromount-G™ Slide Mounting medium (Southern Biotech, Birmingham, AL, USA). Images were taken using a Zeiss LSM 510 confocal microscope (magnification ×800; Zeiss AG, Thornwood, NY, USA) and were analyzed using an Image Analysis system, version 11.0 (Chang Heng Rong Technology Co., Ltd., Beijing; China).
3
Immunohistochemical Analysis of Liver Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissues were fixed in 4% paraformaldehyde and then dehydrated with gradient ethanol and xylene. The sections were then incubated in 1% triton X‐100 for 15 minutes. After eliminating endogenous peroxidase activity with 3% hydrogen peroxide for 15 minutes, the sections were incubated with primary anti‐F4/80 (cat. no. ab6640; 1:100; Abcam Inc) and anti‐TIM‐4 (cat. no. GTX14149; 1:1000; GeneTex Inc) at 4℃ overnight. Next, the sections were washed with PBS and incubated with species‐matched secondary antibodies for 30 minutes at room temperature. The sections were then washed with PBS and sealed with FluorFluoromount‐G™ slide mounting medium (Southern Biotech, Birmingham, AL, USA). Images were acquired using a fluorescence microscope and were analysed using an Image Analysis system, version 11.0 (Chang Heng Rong Technology, Beijing, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!