Gtx109637

Manufactured by GeneTex

Sourced in United States

GTX109637 is a laboratory equipment product. It is a device used for performing experiments and analysis in a research setting. The core function of this product is to provide a tool for researchers to conduct their work. No further details can be provided in an unbiased and factual manner.

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Lab products found in correlation

Dfc345 fx camera, by Leica (1 mentions) Dm4000b microscope, by Leica (1 mentions) Vacht, by Synaptic Systems (1 mentions) Ab47003, by Abcam (1 mentions) Laser confocal microscope, by Leica (1 mentions) Alexa 405 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) N5389, by Merck Group (1 mentions)

2 protocols using gtx109637

Quantifying Neuronal and Cholinergic Innervation

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Cryosections of 12 µm thickness were processed as described previously for human biopsies. Primary antibodies used were PGP 9.5 (1:500 dilution. GTX109637; GeneTEx); and VAChT (1:100 dilution, Synaptic Systems). PGP 9.5 is characterized and consolidated in the literature as a specific pan‐neuronal marker in multiple studies, especially useful for the staining of peripheral neuronal fibers.²⁹, ³⁰ VAChT is consolidated in literature to assess cholinergic innervation. This specific antibody was validated in several studies in the literature.³¹, ³², ³³Images were acquired using Leica DM4000 B microscope coupled with Leica DFC 345FX camera. Quantification was made using ImageJ software (NIH), where 5 airways from each animal were analyzed for positive staining area (mm2) and adjusted by basement membrane area (mm²). Both acquisition and quantification of the images were performed blindly.

Dragunas G., Woest M.E., Nijboer S., Bos S.T., van Asselt J., de Groot A.P., Vohlídalová E., Vermeulen C.J., Ditz B., Vonk J.M., Koppelman G.H., van den Berge M., ten Hacken N.H., Timens W., Munhoz C.D., Prakash Y.S., Gosens R, & Kistemaker L.E. (2020). Cholinergic neuroplasticity in asthma driven by TrkB signaling. The FASEB Journal, 34(6), 7703-7717.

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Immunohistochemical Detection of Neuronal and Cardiac Markers

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All the slides were taken out and recovered to room temperature. After being blocked in 10% normal donkey serum for 1 h, T1-T4 DRGs were incubated with mouse anti-Neurofilament 200 (NF200, 1:100, N5389, Sigma-Aldrich, St. Louis, MO, USA) while the heart sections were treated with either rabbit anti-Tn I (1:100, ab47003, Abcam, Cambridge, MA, USA) or rabbit anti-protein gene product 9.5 (PGP9.5, 1:500, GTX109637, GeneTex, Irvine, CA, USA). Heart and DRG sections were left overnight at 4°C. Sections were washed and incubated with secondary antibodies of Alexa 405-conjugated goat anti-mouse or Alexa 405-conjugated goat anti-rabbit (1:200, Invitrogen, Carlsbad, CA, USA) for 1 h at room temperature. Slides were sealed with a coverslip.
All slides were observed on a Leica laser confocal microscope under 10X and 40X objective and images were captured using a digital camera system. Z-Stacks were acquired for NF200 immunostained DRG slides.

Hong J., Lisco A.M., Rudebush T.L., Yu L., Gao L., Kitzerow O., Zucker I.H, & Wang H.J. (2020). Identification of Cardiac Expression Pattern of Transient Receptor Potential Vanilloid Type 1 (TRPV1) Receptor using a Transgenic Reporter Mouse Model. Neuroscience letters, 737, 135320.

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