Mqae probe

4T1 cells (3 × 10⁵ cells) were seeded in confocal petri dish. After adherence, cells were treated with different formulation (CDDP, F68@TA, F68@TA@Ag, and AINR) for 18 h. In this experiment, the equivalent concentration of CDDP was set as 1 μg/mL. At the end of treatment, cells were washed by PBS, and stained with MQAE probe (5 mM, Beyotime Biotech) for 1 h at 37 ℃. CLSM was used to observe the intracellular fluorescence with the excitation of 405 nm and emission wavelength of 420–480 nm. Mean fluorescence intensity was analyzed by Image J.
For Fe²⁺ detection, cells were stained with Ferro Orange probe (1 μM, Tongren Biotech) for 30 min at 37 ℃. CLSM was used to observe the intracellular fluorescence with the excitation of 552 nm and emission wavelength of 570–620 nm. Mean fluorescence intensity was analyzed by Image J.

Tannic acid (TA), Pluronic® F68, cisplatin (CDDP) and Rhodamine B (RB) were purchased from Yuanye biotechnology company (Shanghai, China). Silver nitrate was obtained from aladdin reagent Co., Ltd. (Shanghai, China). Sodium Dodecyl Sulfonate (SDS) was bought from Sigma-Aldrich (USA). Phosphorylated H2AX (γH2AX) antibody was supplied by Cell Signaling Technology company. NOX4, and NAPDH antibody were purchased from Proteintech company. H₂O₂ fluorescent probe BES H₂O₂-Ac was got from Wako (Cat. #021-17801 (1 mg), Japan). MQAE probe and Fe²⁺ probe (Ferro Orange) were bought from Beyotime company (Shanghai, China) and Dong Ren company (Shanghai, China), respectively.