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Rosettesep human mesenchymal stem cell enrichment cocktail

Manufactured by STEMCELL
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The RosetteSep Human Mesenchymal Stem Cell Enrichment Cocktail is a cell separation product designed to enrich mesenchymal stem cells from human samples. It is a mixture of antibodies that crosslink unwanted cells to RosetteSep densification medium, allowing the target cells to be isolated by centrifugation.

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Lab products found in correlation

Ccd 1140sk, by American Type Culture Collection (2 mentions) Mrc 5, by American Type Culture Collection (2 mentions) Human mda mb231 and mcf7 breast cancer, by American Type Culture Collection (2 mentions) Ficoll paque premium, by GE Healthcare (1 mentions) Ficoll paque medium, by GE Healthcare (1 mentions)

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RosetteSep (157 citations)

5 protocols using rosettesep human mesenchymal stem cell enrichment cocktail

1

Isolation of Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) were isolated from whole blood from five healthy donors (all enrolled in the study after signing on informed consent and post approval from our Institutional Review Board) with RosetteSep Human Mesenchymal Stem Cell Enrichment Cocktail (Stem Cell Technologies, Vancouver, BC, Canada). MSCs were cultured under the same conditions as TDECs.
Zhang Y.K., Wang H., Guo Y.W, & Yue Y. (2019). Novel role of Snail 1 in promoting tumor neoangiogenesis. Bioscience Reports, 39(5), BSR20182161.
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2

Isolation of Bone Marrow-Derived Mesenchymal Stem Cells

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In total, bone marrow samples (60 mL) were aspirated from the iliac crest bone of consenting healthy adult donors (n = 41, median age 25 years, and range 19–35). The study was approved by the local ethics committee. Bone marrow mononuclear cells (BM-MNCs) were isolated by density gradient centrifugation (Ficoll-Paque Premium; GE Healthcare Life Sciences) following incubation with RosetteSep Human Mesenchymal Stem Cell Enrichment Cocktail (StemCell Technologies) for lineage depletion (CD3, CD14, CD19, CD38, CD66b, and glycophorin A).
Ghazanfari R., Li H., Zacharaki D., Lim H.C, & Scheding S. (2016). Human Non-Hematopoietic CD271pos/CD140alow/neg Bone Marrow Stroma Cells Fulfill Stringent Stem Cell Criteria in Serial Transplantations. Stem Cells and Development, 25(21), 1652-1658.
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3

Isolation of Bone Marrow-Derived Mononuclear Cells

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Bone marrow samples were aspirated from the iliac crest of healthy donors (60 ml, n = 21, median age 25, range 18–45). All participants in the study gave a written informed consent. The procedure was approved by the Swedish local ethics committee in Lund (Regionala Etikprövningsnämden Lund, EPN, protocol Dnr2009/532) and all experimental protocols were performed according to EPN’s guidelines. Bone marrow mononuclear cells (BM-MNCs) were isolated by density gradient centrifugation (Ficoll-paque medium, GE Healthcare Life Sciences) combined with prior incubation with RosetteSep Human Mesenchymal Stem Cell Enrichment Cocktail (StemCell Technologies) for lineage depletion (CD3, CD14, CD19, CD38, CD66b and glycophorin A).
Ghazanfari R., Zacharaki D., Li H., Ching Lim H., Soneji S, & Scheding S. (2017). Human Primary Bone Marrow Mesenchymal Stromal Cells and Their in vitro Progenies Display Distinct Transcriptional Profile Signatures. Scientific Reports, 7, 10338.
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Diverse Cell Lines and Knockdown Models

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Human MSCs (CD105+/CD73+/CD44+ and CD34−/CD45−/CD14−/HLA−DR−; Texas A&M Institute for Regenerative Medicine, Bryan, TX) were maintained in MEMα with 20% FBS; a subset was GFP+. Transduction with MZF1 siRNA generated MSCs with constitutive knockdown of MZF1 (MSC(dMZF1)) for use in animal studies. After IRB approval, primary human MSCs (BM-MSCs) were isolated using RosetteSep Human Mesenchymal Stem Cell Enrichment Cocktail (STEMCELL, Vancouver, Canada) from normal human BM isolates. Human MDA-MB231 and MCF7 breast cancer cell lines, purchased from American Type Culture Collection (ATCC, Manassas, VA), were maintained in DMEM with 10% FBS. Lentiviral transduction (GenTarget Inc, San Diego, CA) was used to generate dual-labeled RFP-Luc-MB231 and RFP-Luc-MCF7 cells for use in animal studies. Further manipulation using lentiviral transduction generated MCF7 cells that constitutively express OPN (RFP-Luc-MCF7-lvOPN). Human skin fibroblasts (CCD-1140SK), human lung fibroblasts (MRC-5), and murine embryonic fibroblasts (CF-1) were purchased from ATCC. Human mammary fibroblasts were a gift from Dr. Kuperwasser, Tufts University.
Weber C.E., Kothari A.N., Wai P.Y., Li N.Y., Driver J., Zapf M.A., Franzen C.A., Gupta G.N., Osipo C., Zlobin A., Syn W.K., Zhang J., Kuo P.C, & Mi Z. (2014). Osteopontin Mediates an MZF1-TGF-β1-Dependent Transformation of Mesenchymal Stem Cells into Cancer Associated Fibroblasts in Breast Cancer. Oncogene, 34(37), 4821-4833.
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5

Diverse Cell Lines and Knockdown Models

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Human MSCs (CD105+/CD73+/CD44+ and CD34−/CD45−/CD14−/HLA−DR−; Texas A&M Institute for Regenerative Medicine, Bryan, TX) were maintained in MEMα with 20% FBS; a subset was GFP+. Transduction with MZF1 siRNA generated MSCs with constitutive knockdown of MZF1 (MSC(dMZF1)) for use in animal studies. After IRB approval, primary human MSCs (BM-MSCs) were isolated using RosetteSep Human Mesenchymal Stem Cell Enrichment Cocktail (STEMCELL, Vancouver, Canada) from normal human BM isolates. Human MDA-MB231 and MCF7 breast cancer cell lines, purchased from American Type Culture Collection (ATCC, Manassas, VA), were maintained in DMEM with 10% FBS. Lentiviral transduction (GenTarget Inc, San Diego, CA) was used to generate dual-labeled RFP-Luc-MB231 and RFP-Luc-MCF7 cells for use in animal studies. Further manipulation using lentiviral transduction generated MCF7 cells that constitutively express OPN (RFP-Luc-MCF7-lvOPN). Human skin fibroblasts (CCD-1140SK), human lung fibroblasts (MRC-5), and murine embryonic fibroblasts (CF-1) were purchased from ATCC. Human mammary fibroblasts were a gift from Dr. Kuperwasser, Tufts University.
Weber C.E., Kothari A.N., Wai P.Y., Li N.Y., Driver J., Zapf M.A., Franzen C.A., Gupta G.N., Osipo C., Zlobin A., Syn W.K., Zhang J., Kuo P.C, & Mi Z. (2014). Osteopontin Mediates an MZF1-TGF-β1-Dependent Transformation of Mesenchymal Stem Cells into Cancer Associated Fibroblasts in Breast Cancer. Oncogene, 34(37), 4821-4833.
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