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E 100

Manufactured by Alomone
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The E-100 is a versatile laboratory equipment designed for various scientific applications. It functions as a high-performance electrophoresis system, capable of separating and analyzing biomolecules such as proteins, nucleic acids, and other macromolecules.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (1 mentions) Neurobasal a media, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Gentle cell dissociation reagent (gcdr), by STEMCELL (1 mentions) Dorsomorphin, by Thermo Fisher Scientific (1 mentions) N 260, by Alomone (1 mentions) B 250, by Alomone (1 mentions) Neurocult sm1, by STEMCELL (1 mentions) Mtesr plus, by STEMCELL (1 mentions) Lgx818, by MedChemExpress (1 mentions) Gsk2118436a, by Selleck Chemicals (1 mentions) Plx4032, by MedChemExpress (1 mentions) Plx8394, by MedChemExpress (1 mentions)

3 protocols using e 100

1

Pervanadate Treatment of Serum-Starved Cells

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Unless otherwise indicated, experiments were performed in ∼90% confluent cells cultured in regular cell culture medium. When indicated, cells were serum-starved overnight in medium containing 0.5% fetal bovine serum. Cells were treated for 15 min with 100 μM pervanadate, prepared according to a published protocol (63 (link)). When indicated, cells were incubated for ∼18 h with 5 μM PP2, an SFK inhibitor (Merck Millipore). In some experiments, serum-starved cells were treated for 15 min with 40 ng/ml recombinant human EGF (#E-100, Alomone Labs) alone, or in combination with 100 μM pervanadate.
Sontag J.M., Schuhmacher D., Taleski G., Jordan A., Khan S., Hoffman A., Gomez R.J., Mazalouskas M.D., Hanks S.K., Spiller B.W., Sontag E, & Wadzinski B.E. (2022). A new paradigm for regulation of protein phosphatase 2A function via Src and Fyn kinase–mediated tyrosine phosphorylation. The Journal of Biological Chemistry, 298(8), 102248.
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2

Efficient Generation of iPSC-Derived Organoids

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On day 0 of organoid production, induced pluripotent stem cells (iPSCs), at ~ 80% confluence, were dissociated into small clumps with gentle cell dissociation reagent (StemCell Technologies, 07174) and plated into round-bottom, low-attachment 96 well plates (ThermoFisher, 174925; ~ 15,000 cells per well). Initial media, consisting of mTeSR Plus (Stem Cell Technologies, 05825) supplemented with 5 µm A-83 (Peprotech, 9094360) and 5 µm Dorsomorphin (Peprotech, 8666430), was replaced every other day starting at day 2 by removing half of the media in each well (100 µL) and replacing with fresh media three times. On day 8, media was changed to a base media of Neurobasal A media (Gibco, 10888022) with NeuroCult SM1 (StemCell Technologies, 05711), glutamax (Gibco, 35050061), and Pen/strep (Gibco, 15140122) supplemented with EGF (20 ng/ml; Alomone Labs, E-100) and FGF2 (20 ng/ml; Alomone Labs, F-170). Media was similarly exchanged every other day until day 20 when the mitogenic supplements were exchanged for differentiating factors: BDNF (20 ng/ml; Alomone Labs, B-250) and NT3 (20 ng/ml; Alomone Labs, N-260) in the same base media. After two weeks in differentiation media, organoids could be maintained in the base media for > 100 days41 (link).
Jermakowicz A.M., Rybin M.J., Suter R.K., Sarkaria J.N., Zeier Z., Feng Y, & Ayad N.G. (2021). The novel BET inhibitor UM-002 reduces glioblastoma cell proliferation and invasion. Scientific Reports, 11, 23370.
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3

Evaluating BRAF inhibitor combinations

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The reagents used included PLX4032 (vemurafenib; MedChemExpress, HY-12057), LGX818 (encorafenib; MedChemExpress, HY-15605), GSK2118436A (dabrafenib; Selleckchem, S2807), PLX8394 (MedChemExpress, HY-18972), Benzyl-coelenterazine (Nanolight, 301), and human EGF (Alomone Labs, E-100).
Mayrhofer J.E., Enzler F., Feichtner A., Röck R., Fleischmann J., Raffeiner A., Tschaikner P., Ogris E., Huber R.G., Hartl M., Schneider R., Troppmair J., Torres-Quesada O, & Stefan E. (2020). Mutation-oriented profiling of autoinhibitory kinase conformations predicts RAF inhibitor efficacies. Proceedings of the National Academy of Sciences of the United States of America, 117(49), 31105-31113.
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