Anti cd73 apc

Manufactured by BioLegend

Sourced in United States

Anti-CD73-APC is a fluorescently conjugated antibody that binds to the CD73 protein. CD73 is an ectoenzyme that catalyzes the conversion of extracellular adenosine monophosphate (AMP) to adenosine. This antibody can be used to detect and quantify CD73-expressing cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

APC anti-human CD73 APC-conjugated anti-CD73 Anti-human CD73 (APC/Cy7) APC anti-human CD73 clone AD2 CD73-APC Anti-CD73

6 protocols using anti cd73 apc

Quantification of CCL5 in HCC, Cirrhosis, and Healthy Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human CCL5 concentration in the serum of the HCC patients, liver cirrhosis patients and healthy people as well as in the CM of CAFs and PTFs were determined with CCL5 ELISA kit (P13501, RayBiotech) under the guidance of instruction. The following antibodies were used for cytometry: anti-CD105-FITC (800505, Biolegend, San Diego, CA, USA), anti-CD73-APC (344005, Biolegend), anti-CD90-FITC (328170, Biolegend), anti-CD44-FITC (338803, Biolegend), anti-CD34-FITC (343503, Biolegend), anti-CD45-FITC (982316, Biolegend), anti-HLA-DR-FITC (980402, Biolegend) and anti-CD31-APC (303115, Biolegend). The cells staining was performed under manufacturer’s protocol and identified by a flow cytometer (FACSCanto II, BD, USA). FlowJo software (Version X; TreeStar, Ashland, OR, USA) was used to data analysis.

Xu H., Zhao J., Li J., Zhu Z., Cui Z., Liu R., Lu R., Yao Z, & Xu Q. (2022). Cancer associated fibroblast–derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis. Cell Death & Disease, 13(5), 478.

+ Open protocol

+ Expand

Immunophenotypic Characterization of BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunophenotypic characterization of cells, 1.0 × 10⁶ BMSCs per tube were washed with FACS buffer consisting of PBS supplemented with 1% bovine serum albumin (BSA, Sigma). Then, the cells were incubated for 30 minutes in the dark at room temperature with the following fluorochrome-conjugated primary antibodies: anti-CD90-Percp-Cy5.5, anti-CD73-APC, anti-CD105-FITC, anti-CD146-PE (all from BioLegend, San Diego, CA, USA), anti-CD14-FITC (Immunostep, Salamanca, SPA), anti-CD34-FITC, anti-CD45-Percp-Cy5.5 (both from Agilent DAKO, Santa Clara, CA, USA), and anti-CD11b-PE (Santa Cruz Biotechnology, Dallas, TX, USA). The isotype controls were IgG2A-FITC, IgG1A-APC, IgG1A-Percp-Cy5.5, IgG1-PE, IgG1-FITC, and IgG2A-PE (all from Santa Cruz Biotechnology). Next, the cells were washed with FACS buffer and resuspended in 300 μL buffer for acquisition with a BD FACSCanto™ cytometer (BD Biosciences). The data were analyzed with FlowJo software (Tree Star, Ashland, OR, USA).

Dias R.B., Guimarães J.A., Cury M.B., Rocha L.R., da Costa E.S., Nogueira L.P., Hochman-Mendez C., Fortuna-Costa A., Silva A.K., Cunha K.S., de Souza S.A., Duarte M.E., Sartore R.C, & Bonfim D.C. (2019). The Manufacture of GMP-Grade Bone Marrow Stromal Cells with Validated In Vivo Bone-Forming Potential in an Orthopedic Clinical Center in Brazil. Stem Cells International, 2019, 2608482.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Analysis of Liver and Spleen Lymphocytes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymphocytes from the liver and spleen of mice were isolated as previously described²³ (link) and analyzed by multicolor flow cytometry. Flow cytometry was performed on a CytoFLEX LX platform and results were analyzed using FlowJo software. The following antibodies were used in this study (clone, manufacture): anti-F4/80-Alexa Fluor 700 (BM8, BioLegend), anti-B220-Alexa Fluor 700 (RA3-6B2, BioLegend), anti-Cd11b-Alexa Fluor 700 (M1/70, BioLegend), anti-Cd3-Alexa Fluor 594 (17A2, BioLegend) anti-Cd4-BV605 (GK1.5, BioLegend), anti-Cd8-BV786 (53-6.7, BD), anti-Foxp3-BV421 (MF-14, BioLegend), anti-Cd62l-PerCP/Cy5.5 (MEL-14, BioLegend), anti-Cd44-BV510 (IM7, BioLegend), anti-NK1.1-BV510 (PK136, BioLegend), anti-A2Ar-FITC (7F6-G5-A2, NOVUS), anti-Cd73-APC (TY11.8, BioLegend), anti-Cd39-PECy7 (Duha59, BioLegend), anti-Cd19-PerCP Cy5.5(6D5, BioLegend), anti-PD1-APC/Cy7 (29F.1A12, BioLegend), anti-Cd107a- PE (1D4B, BD), anti-CD69-BV650 (H1.2F3, BioLegend), anti-Ifnγ-BV421 (XMG1.2, BioLegend), anti-Tnfα-PerCPCy5.5 (MP6-XT22, BioLegend), anti-GZMB-FITC (GB11, BioLegend), and anti-perforin-APC (S16009A, BioLegend).

Myojin Y., McCallen J.D., Ma C., Bauer K.C., Ruf B., Benmebarek M.R., Green B.L., Wabitsch S., McVey J.C., Fu C., Xie C, & Greten T.F. (2023). Adenosine A2a receptor inhibition increases the anti-tumor efficacy of anti-PD1 treatment in murine hepatobiliary cancers. JHEP Reports, 6(1), 100959.

+ Open protocol

+ Expand

Flow Cytometric Analysis of MSC Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometric analysis was performed to confirm the MSCs surface markers. Two cell lines of hWJ-MSCs were performed. The adherent MSCs cells were treated with trypsin-EDTA and collected after being centrifuged at 350× g for 5 min. MSCs were incubated in antibodies in the dark for 20 min. All antibodies are listed in Table S1. Each antibody was diluted in 100 mL of PBS(-)—the following antibodies: anti-CD34-PE (dilution 1:10, Beckman Coulter, Brea, CA, USA), anti-CD45-FITC (dilution 1:20, Biolegend, San Diego, CA, USA), anti-CD73-APC, anti-CD90-APC/A750, and anti-CD105-PE (dilution 1:100, Biolegend). The samples were analyzed by an Attune™ NxT Flow Cytometer (Attune™ NXT, Thermo Fisher Scientific, Cleveland, OH, USA). The percentage of CD34-, CD45-, CD73+, CD90+, and CD105+ positive or negative cell populations were calculated using the FCS Express™ Software.

Somredngan S., Theerakittayakorn K., Nguyen H.T., Ngernsoungnern A., Ngernsoungnern P., Sritangos P., Ketudat-Cairns M., Imsoonthornruksa S., Keeratibharat N., Wongsan R., Rungsiwiwut R, & Parnpai R. (2023). The Efficiency of Neurospheres Derived from Human Wharton’s Jelly Mesenchymal Stem Cells for Spinal Cord Injury Regeneration in Rats. International Journal of Molecular Sciences, 24(4), 3846.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following fluorochrome-coupled versions of these antibodies were used in this study. The number in brackets indicates the manufacturer´s reference.
Purchased from BD Pharmigen: PE-anti-CD3ε (553064), PerCP-C5.5-anti-IL-17A (560666), FITC-anti-CD122 (553361), BV605-anti-Vγ2 TCR (742310), Biotin-anti-CD4 (553045), PE-anti-CD45.2 (560695), FITC-anti-Ly-6C (553104), PerCP-C5.5- anti-CD64 (561194), PE-Cy7-anti-Ly-6G (560601), APC-anti-CD11c (550261), Biotin-anti-CD11b (553309), FITC-anti-CD24 (553261), BV605-Streptavidin (563260) and PE-C7-Streptavidin (557598). From eBioscience/Invitrogen: PerCP-eFluor710-anti-TCRγ/δ (46-5711-82), APC-anti-CD27 (17-0271-82), PE-Cy7-anti-TCR Vγ2 (25-5828-82), APC-anti-RORγt (17-6988-82), and Biotin-anti-CD8a (13-0081-85). From Biolegend: BV421-anti-CD44 (103040), BV421-anti-TCRγδ (118119), PE/Cy7-anti-CD27 (124216), APC-anti-CD45RB (103320), APC-anti-CD73 (127210) and BV421-Streptavidin (405225). From Miltenyi Biotec: APC-anti-IFNγ (130-120-805).

Borroto A., Alarcón B, & Navarro M.N. (2022). Mutation of the Polyproline Sequence in CD3ε Evidences TCR Signaling Requirements for Differentiation and Function of Pro-Inflammatory Tγδ17 Cells. Frontiers in Immunology, 13, 799919.

+ Open protocol

+ Expand

Characterization of Third-Generation hDPSC

Check if the same lab product or an alternative is used in the 5 most similar protocols

After isolation and culture, the third-generation stem cells were collected and analyzed by flow cytometry using BD FACSMelody (BD Biosciences, San Diego, USA). Flowjo_v10 software was used to analyze the data. Thereby, FITC anti-CD90 (cat 328107), PE anti-CD105 (cat 800503), APC anti-CD73 (cat 344005), PerCP/Cy5.5 anti-CD34 (cat 343521), and APC/FireTM750 anti-CD45 (cat 368517) antibody (BioLegend, USA) stains were assessed for third-generation hDPSC.

Shang W, & Xiong S. (2022). Phenytoin Is Promoting the Differentiation of Dental Pulp Stem Cells into the Direction of Odontogenesis/Osteogenesis by Activating BMP4/Smad Pathway. Disease Markers, 2022, 7286645.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!