Click it plus 5 ethynyl 2 deoxyuridine edu flow cytometry assay kit

Manufactured by Thermo Fisher Scientific

The Click-iT Plus 5-ethynyl-2'-deoxyuridine (EdU) Flow Cytometry Assay kit is a tool used for the detection and quantification of cellular proliferation. It enables the incorporation of EdU, a thymidine analog, into newly synthesized DNA during the S-phase of the cell cycle. The incorporated EdU can then be detected and visualized using a copper-catalyzed click reaction with a fluorescent dye.

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Lab products found in correlation

Pcdna3, by Promega (3 mentions) Pcdna3.1 nfat2, by Promega (3 mentions) Pluronic f 127, by GeneCopoeia (3 mentions) Hrp conjugated secondary antibody, by Bioss Antibodies (3 mentions) C myc, by Cell Signaling Technology (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Fitc annexin 5 apoptosis detection kit, by BD (3 mentions) Reagents for cell culture, by Thermo Fisher Scientific (2 mentions)

3 protocols using click it plus 5 ethynyl 2 deoxyuridine edu flow cytometry assay kit

Plasmid Transfection and Molecular Assays

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The expression plasmids pcDNA3.1-NFAT2 and its empty vector pcDNA3.1 were purchased from promega (WI, USA). The Lipofectamine 2000 was obtained from Invitrogen (Thermofisher Scientific., USA). Pluronic F-127 was bought from Genecopoeia (MA, USA). Fluo-4/AM, anhydrous dimethyl sulfoxide (DMSO) and cell counting kit-8 (CCK-8) were acquired from Dojindo Molecular Technologies Inc. (Tokyo, Japan). Antibodies against NFAT2 (RRID: AB_2152507), Egr2 (RRID:AB_1139730), FasL (RRID:AB_302235), ERK (RRID:AB_11157324) and β-actin (RRID:AB_764434) were obtained from Abcam (Cambridge, UK). Antibodies against AKT (RRID:AB_329827), p-AKT(Try326) (RRID:AB_1264114), p-ERK(S189) (RRID:AB_490903), c-myc (RRID:AB_2151827) and Cox-2 (RRID:AB_2084968) were bought from Cell Signaling Technology (MA, USA). The HRP-conjugated secondary antibody was acquired from Bioss, Inc. (Beijing, China). Annexin V-FITC apoptosis detection kit was obtained from BD Biosciences (NJ, USA). The Click-iT Plus 5-ethynyl-2′-deoxyuridine (EdU) Flow Cytometry Assay kit was bought from Invitrogen. All chemical reagents used in this study were of analytical grade and all reagents for cell culture were purchased from Gibco BRL Life Technologies (MD, USA).

Wang J., Zhang Y., Liu L., Cui Z., Shi R., Hou J., Liu Z., Yang L., Wang L, & Li Y. (2020). NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression. BMC Cancer, 20, 966.

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Plasmid Transfection and Cell Signaling Analysis

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The expression plasmids pcDNA3.1-NFAT2 and its empty vector pcDNA3.1 were purchased from promega (WI, USA). The Lipofectamine 2000 was obtained from Invitrogen (Thermo sher Scienti c., USA). Pluronic F-127 was bought from Genecopoeia (MA, USA). Fluo-4/AM, anhydrous dimethyl sulfoxide (DMSO) and cell counting kit-8 (CCK-8) were acquired from Dojindo Molecular Technologies Inc. (Tokyo, Japan). Antibodies against NFAT2 (RRID: AB_2152507), Egr2 (RRID:AB_1139730), FasL (RRID:AB_302235), ERK (RRID:AB_11157324) and β-actin (RRID:AB_764434) were obtained from Abcam (Cambridge, UK). Antibodies against AKT (RRID:AB_329827), p-AKT(Try326) (RRID:AB_1264114), p-ERK(S189) (RRID:AB_490903), c-myc (RRID:AB_2151827) and Cox-2 (RRID:AB_2084968) were bought from Cell Signaling Technology (MA, USA). The HRP-conjugated secondary antibody was acquired from Bioss, Inc. (Beijing, China). Annexin V-FITC apoptosis detection kit was obtained from BD Biosciences (NJ, USA). The Click-iT Plus 5-ethynyl-2'-deoxyuridine (EdU) Flow Cytometry Assay kit was bought from Invitrogen. All chemical reagents used in this study were of analytical grade and all reagents for cell culture were purchased from Gibco BRL Life Technologies (MD, USA).

Wang J., Zhang X., Liu L., Cui Z., Shi R., Hou J., Liu Z., Yang L., Wang L., & Wang J. (2020). NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression.

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NFAT2 Overexpression in Cell Assays

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The expression plasmids pcDNA3.1-NFAT2 and its empty vector pcDNA3.1 were purchased from promega (WI, USA). The Lipofectamine 2000 was obtained from Invitrogen (Thermo sher Scienti c., USA). Pluronic F-127 was bought from Genecopoeia (MA, USA). Fluo-4/AM, anhydrous dimethyl sulfoxide (DMSO) and cell counting kit-8 (CCK-8) were acquired from Dojindo Molecular Technologies Inc. (Tokyo, Japan). Antibodies against NFAT2 (RRID:AB_2152507), Egr2 (RRID:AB_1139730), FasL (RRID:AB_302235), ERK (RRID:AB_11157324) and β-actin (RRID:AB_764434) were obtained from Abcam (Cambridge, UK). Antibodies against AKT (RRID:AB_329827), p-AKT(Try326) (RRID:AB_1264114), p-ERK(S189) (RRID:AB_490903), c-myc (RRID:AB_2151827) and Cox-2 (RRID:AB_2084968) were bought from Cell Signaling Technology (MA, USA). The HRP-conjugated secondary antibody was acquired from Bioss, Inc. (Beijing, China). Annexin V-FITC apoptosis detection kit was obtained from BD Biosciences (NJ, USA). The Click-iT Plus 5-ethynyl-2'-deoxyuridine (EdU) Flow Cytometry Assay kit was bought from Invitrogen. All chemical reagents used in this study were of analytical grade and all reagents for cell cultures were purchased from Gibco BRL Life Technologies (MD, USA).

Wang J., Zhang X., Liu L., Cui Z., Shi R., Hou J., Liu Z., Yang L., Wang L., & Li Y. (2020). NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression.

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