Ms grade methanol

Manufactured by Merck Group

Sourced in Germany, United States

MS-grade methanol is a high-purity solvent used in analytical laboratory applications, particularly in mass spectrometry (MS) techniques. This solvent meets the stringent requirements for use in MS instrumentation, ensuring consistent and reliable performance. MS-grade methanol is designed to minimize contaminants and impurities that could interfere with sensitive MS analyses.

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Lab products found in correlation

Formic acid, by Merck Group (14 mentions) Acetonitrile, by Merck Group (11 mentions) Ms grade acetonitrile, by Merck Group (3 mentions) Ammonium acetate, by Merck Group (2 mentions) Milli q system, by Merck Group (2 mentions) Water purification system, by Thermo Fisher Scientific (2 mentions) Potassium dichromate k2cr2o7, by Merck Group (2 mentions) Ms grade ammonium formate, by Merck Group (2 mentions) Quinoxaline, by Merck Group (2 mentions) 2 methylquinoxaline, by Merck Group (2 mentions) Sodium borate, by Sinopharm (2 mentions) Cml and cel standards, by LGC (2 mentions) Sodium borohydride, by Sinopharm (2 mentions) Hydrochloric acid (hcl), by Sinopharm (2 mentions) Chloroform, by Sinopharm (2 mentions) Sodium hydroxide (naoh), by Sinopharm (2 mentions) Boric acid, by Sinopharm (2 mentions) Amazon sl quadrupole ion trap mass spectrometer, by Bruker (1 mentions) Bisphenol f, by Merck Group (1 mentions) Bisphenol af, by Merck Group (1 mentions)

26 protocols using ms grade methanol

Quantitative Analysis of Alkyl Ethoxylates

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Individual alcohol ethoxylates C₁₂EO_X (x = 1; 2; 3; 4; 5; 6; 7; 8; 9) and C₁₂OH used as standards were obtained from Fluka (Germany), and C₁₂EO₃ was from Sigma-Aldrich (ST. Louis, MO, USA). All standards were of high purity grade (>98%). MS-grade acetonitrile and MS-grade methanol were from Sigma-Aldrich (ST. Louis, MO, USA). Ammonium acetate used as mobile phase additive was purchased from Sigma-Aldrich (ST. Louis, MO, USA). Water was prepared by reverse osmosis in a Demiwa system via double distillation in a quartz apparatus. Only freshly distilled water was used. Phenyl isocyanate (>99%) used as a derivatization agent was purchased from Fluka (Germany).
Ethyl acetate, sodium chloride and hydrogen carbonate (purchased from POCh Gliwice Poland) used for liquid–liquid extraction (LLE) were of analytical grade.
Standard stock solutions (of each compound) were prepared in acetonitrile and kept at 4 °C.

, & Zembrzuska J. (2017). Determination of Dodecanol and Short-Chained Ethoxylated Dodecanols by LC–MS/MS (with Electrospray Ionization) After Their Derivatization (with Phenyl Isocyanate). Journal of Surfactants and Detergents, 20(6), 1421-1432.

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Bacterial Lipid Extraction and Characterization

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Lipids were extracted from bacterial cultures as described elsewhere^{62 (link)}. Briefly, bacteria were grown to OD₆₀₀ of 1.0 in TSB or TSB supplemented with 10 µM LA, centrifuged in a 2 mL glass Chromacol vial (Thermo Scientific), and resuspended in 0.5 mL MS grade methanol (Sigma-Aldrich). MS grade chloroform was then used to extract lipids. The extracted lipids were dried under nitrogen gas with a Techne sample concentrator (Staffordshire, UK), and the lipid pellets resuspended in 1 mL acetonitrile. The samples were then analysed by LC–MS with a Dionex 3400RS HPLC coupled to an amaZon SL quadrupole ion trap mass spectrometer (Bruker Scientific) via an electrospray ionisation interface. Both positive and negative ionisation modes were used for sample analyses. The Bruker Compass software package was utilized for data analyses, using DataAnalysis for peak identification and characterization of lipid class, and QuantAnalysis for quantification of the relative abundance of distinct PG species to total PG species.

Kengmo Tchoupa A., Watkins K.E., Jones R.A., Kuroki A., Alam M.T., Perrier S., Chen Y, & Unnikrishnan M. (2020). The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids. Scientific Reports, 10, 14838.

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Bisphenol Derivatives Analysis Protocol

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Standards of bisphenol A, bisphenol F, bisphenol S, bisphenol AF, bisphenol E, and bisphenol B (Fig. 1) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and were each of > 99% purity. MS-grade methanol was purchased from Sigma-Aldrich, and MS-grade acetonitrile was from POCh (Gliwice, Poland). As an additive to HPLC, mobile phase ammonium acetate ≥ 99% (Fluka Analytical, Switzerland) was used. The HPLC-grade water was prepared by reverse osmosis in a Demiwa system from Watek (Ledec nad Sazavou, Czech Republic), followed by double distillation from a quartz apparatus. The yellow tetrazole dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) used in the MTT assay was purchased from Sigma-Aldrich. All salts used for preparation of media were purchased from Avantor (Gliwice, Poland).

Frankowski R., Zgoła-Grześkowiak A., Smułek W, & Grześkowiak T. (2020). Removal of Bisphenol A and Its Potential Substitutes by Biodegradation. Applied Biochemistry and Biotechnology, 191(3), 1100-1110.

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Quantification and Characterization of Polyphenolic Compounds in White Grape Extract

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The standards used for the quantification of bioactivities as well as the individual characterization of the main polyphenolic compounds contained in the white grape extract, with their CAS numbers, purity, and suppliers are summarized in Supplementary Table 1. The solvents used for the extraction process were ethanol, ethyl lactate, propylene glycol, and ultrapure water of MS grade from Scharlab (Barcelona, Spain). MS-grade methanol obtained from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and formic acid from Merck (Darmstadt, Germany), were used to prepare the mobile phases for chromatographic analysis.

Castillo A., Celeiro M., Rubio L., Bañobre A., Otero-Otero M., Garcia-Jares C, & Lores M. (2022). Optimization of bioactives extraction from grape marc via a medium scale ambient temperature system and stability study. Frontiers in Nutrition, 9, 1008457.

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Carotenoid Extraction and Analysis Protocol

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The solvents used for the extraction process were absolute food grade ethanol (above 99.8%) provided by VWR (Leicestershire, England), ethyl lactate supplied by Fluka Analytical (Steinheim, Germany) and Ultrapure water MS-grade from Scharlab (Barcelona, Spain). For MSPD extraction, sand was used as a dispersant medium with an average particle size of 2 × 10² to 3 × 10² μm Scharlau Chemie S.A. (Barcelona, Spain). For mobile phase preparation in HPLC-DAD and UHPLC-QToF-MS/MS, MS-grade methanol obtained by Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and formic acid obtained by Merck (Darmstadt, Germany) were used. The astaxanthin standard was provided by Biosynth Carbosynth (Berkshire, United Kingdom), β-carotene was supplied by ThermoFisher (Kandel, Germany) and zeaxanthin and lutein were supplied by Extrasynthese (Genay, France).
Standard solutions were prepared in ethanol. The extraction processes and preparation of standards were carried out in a red-light room, in a dry environment. Standards and extracts were stored in amber glass containers, sealed with paraffinic material and kept in a dark and controlled environment at −20 °C. Reagents and samples were stored in different places to avoid cross-contamination.

Castillo A., Pereira S., Otero A., Fiol S., Garcia-Jares C, & Lores M. (2020). Matrix solid-phase dispersion as a greener alternative to obtain bioactive extracts from Haematococcus pluvialis. Characterization by UHPLC-QToF. RSC Advances, 10(47), 27995-28006.

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Estrogen Compound Characterization and Cell Viability Assay

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Mass Spectroscopy (MS) grade methanol, acetonitrile (ACN), water and formic acid (FA) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Fetal bovine serum (FBS) and antibiotics were purchased from GIBCO (Grand Island, NY). The 17β-estradiol benzoate [(Estr-1,3,5(10)-trien-3,16α,17β-triol); purity ≥98%] was obtained from Sigma-Aldrich (Hamburg, Germany). Estradiol valerate (Progynova® 2 mg) was purchased from DELPHARM (Lille, France). The 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid (HEPES, purity ≥99.5%) was purchased from Ludwig Biotecnologia Ltda (Alvorada, RS, Brazil). Trypan blue, Sulforodamine B, Serum Replacement 2 and cell culture mediums were purchased from Sigma-Aldrich (St. Louis, MO, USA). Genistein was obtained from “Extrasynthese®” (Genay, France).

Zingue S., Michel T., Nde C.B., Njuh A.N., Cisilotto J., Ndinteh D.T., Clyne C., Fernandez X., Creczynski-Pasa T.B, & Njamen D. (2017). Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study. BMC Complementary and Alternative Medicine, 17, 383.

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Quantitative Analysis of Metabolites

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MS grade methanol, acetonitrile, isopropanol, and formic acid were purchased from Sigma-Aldrich (St. Louis, MO). Ultrapure water was prepared by the Milli-Q system (Millipore, Billerica, MA). All the reference standards and stable isotope-labeled internal standards were purchased from Sigma-Aldrich (St. Louis, MO), Steraloids Inc. (Newport, RI), TRC Chemicals (Toronto, ON, Canada), and Nu-Chek Prep (Elysian, MN). The derivatization regents including 3-nitrophenylhydrazine (3-NPH) and N-(30(dimethylamino)propyl)-N'-ethylcarbodiimide (EDC) were purchased from Sigma-Aldrich (St. Louis, MO).

Zhu C., Huang F., Li Y., Zhu C., Zhou K., Xie H., Xia L, & Xie G. (2022). Distinct Urinary Metabolic Biomarkers of Human Colorectal Cancer. Disease Markers, 2022, 1758113.

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Antioxidant and Cytoprotective Activity Assays

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Folin-Ciocalteu reagent, 2,2′-azobis-(2-methylpropionamidine) dihydrochloride (AAPH), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), fluorescein, HPLC grade methanol, and MS grade methanol were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Standards of gallic acid, methyl gallate, rutin, and quercetin were also purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Hexanes, sodium hydroxide, hydrochloric acid, and sodium carbonate were purchased from Fisher Scientific (Ottawa, ON, Canada). Hydrogen peroxide (H₂O₂) and 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (Oakville, ON, Canada). Hank’s balanced salt solution (HBSS), dulbecco’s modified eagle medium /F12 (DMEM/F12), anti-anti and fetal bovine serum (FBS) T-75 culture flasks, Transwell Permeable supports (0.6 cm², 0.4 μm pore size), and cell culture plate were purchased from Invitrogen, Fisher Scientific (Ottawa, ON, Canada).

Xiang J., Yang C., Beta T., Liu S, & Yang R. (2019). Phenolic Profile and Antioxidant Activity of the Edible Tree Peony Flower and Underlying Mechanisms of Preventive Effect on H2O2-Induced Oxidative Damage in Caco-2 Cells. Foods, 8(10), 471.

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Physicochemical Profiling of Algerian Date Powder

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The HPLC-grade water was obtained from a LC-Pak™ Millex system (Millipore Corporation, Billerica, MA, USA). Formic acid, MS grade methanol, citric acid, galacturonic acid, malic acid, glucose, sucrose, gallic acid, and caffeine were obtained from Sigma-Aldrich, St. Louis, MO, USA. Quinic acid, catechin, procyanidin B 1 , procyanidin C 1 , kaempferol glucoside, kaempferol, rutin, and quercetin rhamnoside were obtained from PhytoLab, Vestenbergsgreuth, Germany. The ''Degla-Beida'' dates used in this study present a dry nature and a hard texture, that are found in the palm groves of South-East Algeria. The choice of this variety is justified by its relative abundance on the national territory, its low market value, its long shelf life due to its dry nature, its taste and nutritional quality (energy source represented by its high sugar content) (Djaoud et al., 2020) . The dates were washed with tap water followed by distillated water, pitted and deposited in a drying oven at 40°C until up to a constant weight. Then, the dried samples were ground into a fine powder, which was passed through a standard sieve (125 µm diameter) and only the fraction with the particle size≤125 µm was used. Finally, the powder was stored in the darkness (Djaoud et al., 2019) .

Djaoud K., Daglia M., Sokeng A.J.T., Kermiche F., Arkoub L., & Makhlouf L.B. (2020). RP-HPLC-PDA-ESI-MS/MS screening of bioactive compounds from Degla-Beida dates: Conventional and green extraction technologies. The Annals of the University Dunarea de Jos of Galati Fascicle VI – Food Technology, 44(1), 58-81.

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Extraction and Analysis of Liquidambar formosana

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Dried mature inflorescences of Liquidambar formosana Hance was obtained from Anguo Qimei medicinal materials Co., Ltd. (Anguo, China). MS grade methanol and acetonitrile were bought from Merck (Darmstadt, Germany). Diethylnitrosamine (DEN) was provided by Sigma (United States). Rat alpha-fetoprotein (AFP) ELISA kit, Rat alanine transaminase (ALT) ELISA kit, Rat aspartate amino-transferase (AST) ELISA kit, Rat tumor necrosis factor-α (TNF-α) ELISA kit were acquired from Shanghai Langdon Biotechnology Co., Ltd. (Shanghai,China).

Wang S., Yang X.X., Li T.J., Zhao L., Bao Y.R, & Meng X.S. (2022). Analysis of the absorbed constituents and mechanism of liquidambaris fructus extract on hepatocellular carcinoma. Frontiers in Pharmacology, 13, 999935.

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