M365lp1

Manufactured by Thorlabs

Sourced in United States

The M365LP1 is a laser diode module that provides a 365 nm ultraviolet (UV) light output. It is designed for use in various applications that require a compact and reliable UV light source.

Automatically generated - may contain errors

Lab products found in correlation

V 550 spectrophotometer, by Jasco (2 mentions) Flame t vis nir es usb spectrometer, by OceanOptics (2 mentions) Ledd1b t cube led driver, by Thorlabs (2 mentions) Cetuximab, by Selleck Chemicals (1 mentions) Bis tris page gel, by Thermo Fisher Scientific (1 mentions) Amicon ultra 50 kda mwco spin filter columns, by Merck Group (1 mentions) Tensor 27 ftir spectrometer, by Bruker (1 mentions) Cary 50 spectrophotometer, by Agilent Technologies (1 mentions) Wp25m ub, by Thorlabs (1 mentions) Aqwp05m 580, by Thorlabs (1 mentions) Aqwp05m 340, by Thorlabs (1 mentions) Lpvise100 a, by Thorlabs (1 mentions) Cary 5000 spectrophotometer, by Agilent Technologies (1 mentions) Micrometer tilt stage, by Edmund Optics (1 mentions) Tetraspeck microsphere, by Thermo Fisher Scientific (1 mentions) Tobradex, by Alcon (1 mentions) Rm105, by Merck Group (1 mentions) Irgacure 819, by Merck Group (1 mentions) Sm2f32 a, by Thorlabs (1 mentions) Dc4104, by Thorlabs (1 mentions)

Spelling variants of this product

M365LP1-C4 (2 citations)

19 protocols using m365lp1

Photochromic Transient Absorption Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

UV LED (λ_exc = 365 nm, M365LP1, Thorlabs, Newton, New Jersey, USA) was used to perform photochromic reaction in a 1 cm × 1 cm fused silica cuvette placed in a temperature-controlled cuvette holder (Flash 300, Quantum Northwest, Liberty Lake, Washington, USA) with stirring (see Scheme S1). The volume of the solution was about 1.5 mL. Changes in UV-vis absorption spectra over seconds were recorded by a FLAME-T-VIS-NIR-ES USB spectrometer (6 s⁻¹ sampling rate, Ocean Optics, Largo, Florida, USA). White-light of 150 W xenon lamp (Applied Photophysics, Leatherhead, Surrey, United Kingdom) with intensity reduced to a small level was used as a probing beam. 150 ms were used for accumulation of each white-light continuum spectrum. An average of 60 initial spectra before sample UV-irradiation was used to calculate

I_{0} (λ)

spectrum, the next spectra

I (λ)

at subsequent times were measured upon or after UV-irradiation to determine the transient absorption spectra accordingly to formula:

Δ A (λ) = \log \frac{I_{0} (λ)}{I (λ)}

.
In order to study influence of UV irradiation power on TT formation a V-550 spectrophotometer (Jasco, Hachioji-shi, Tokyo) equipped with the mentioned above temperature-controlled cuvette holder, stirring and UV LED source was used.

Brazevic S., Nizinski S., Sliwa M., Abe J., Rode M.F, & Burdzinski G. (2020). Control of the Photo-Isomerization Mechanism in 3H-Naphthopyrans to Prevent Formation of Unwanted Long-Lived Photoproducts. International Journal of Molecular Sciences, 21(21), 7825.

+ Open protocol

+ Expand

Photoconjugation of PpL Mutants and Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

In initial screens, PpL mutants and anti-CD3 (clone UTH1, BD bioscience 555329) antibodies were diluted into PBS pH7.6 such that the final concentrations were approximately 50 µM and 2 µM, respectively, and loaded into thin walled 200 µL polypropylene microtubes (PCR tubes). This mixture was then irradiated for 1 h under 365 nm light at an intensity of 6.4 mW/cm² from an LED source (M365LP1, Thor Labs) 14 cm away. Products were reduced using DTT solution (ThermoFisher) and separated on 4–12% BisTris PAGE gels (ThermoFisher) to observe photoconjugation. Full gel images are shown in Supplementary Fig. S2. Photocleavage was accomplished using the same irradiation setup. Photoconjugations to anti-FLAG antibody (anti-DYDDDDK clone 1557CT661.18.1, Lifespan Biosience LS‑C392574) or Cetuximab (Selleck Chemicals A2000) were done identically, with 100 µM PpL constructs that had been freshly purified. Photoconjugates were then purified from excess PpL using Amicon Ultra 50 kDa MWCO spin filter columns (Millipore-Sigma, UCFC505008). The unaltered antibody control conditions of our ELISA experiments, described below, validated Anti-FLAG binding to the FLAG peptide and Cetuximab binding to EGFR protein.

Brasino M., Wagnell E., Hamilton S., Ranganathan S., Gomes M.M., Branchaud B., Messmer B, & Ibsen S.D. (2022). Turning antibodies off and on again using a covalently tethered blocking peptide. Communications Biology, 5, 1357.

+ Open protocol

+ Expand

Simultaneous UV-vis and IR Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Changes in UV–vis absorption spectra and kinetics were recorded using three configurations.

Jasco V-550 spectrophotometer with a modified cell compartment. The solution in 1 cm × 1 cm fused silica cuvette was placed into a temperature-controlled cuvette holder (Flash 300, Quantum Northwest) with stirring switched on. UV LED (λ_exc = 365 nm, Thorlabs M365LP1) was used to induce the photochromic reaction (as in the two other configurations below).

Similar arrangement with a temperature-controlled cuvette holder and white light generated by a xenon lamp (Applied Photophysics), equipped with a bundle fiber, as a probing beam. The probing beam was passed through an almost-closed iris to ensure low white light intensity. The UV–vis spectra were recorded by an Ocean Optics FLAME-T-VIS–NIR-ES USB spectrometer at the sampling rate of 10 spectra per second.

Acquisition mid-IR spectra upon simultaneous measurements in UV–vis spectral range has been described recently^{11 (link)}. A Bruker Tensor 27 FT-IR spectrometer was equipped with an MCT detector, with a spectral resolution of 4 cm⁻¹ and a sampling rate of 1.15 s⁻¹. The UV–vis probing light was generated from the xenon lamp. An Ocean Optics spectrophotometer was used for recording of UV–vis spectra. A Harrick Scientific cell was used with 2 mm thick CaF₂ window and a 0.63 mm spacer.

Gierczyk B., Rode M.F, & Burdzinski G. (2022). Mechanistic insights into photochromic 3H-naphthopyran showing strong photocoloration. Scientific Reports, 12, 10781.

+ Open protocol

+ Expand

Mouse Model of UV-Induced FECD

Check if the same lab product or an alternative is used in the 5 most similar protocols

The nongenetic FECD mouse model was obtained as previously described.²² (link) Briefly, A UVA light-emitting diode (LED) light source (M365LP1, Thorlabs, USA) was focused to illuminate a 4-mm-diameter spot onto the mouse cornea. The energy was measured using a laser sensor (model L49 150A, Ophir, Israel), and the time of UVA exposure was adjusted to deliver the appropriate fluency (20 min 57 s for 500 J/cm², 31 min 26 s for 750 J/cm², 41 min 54 s for 1,000 J/cm²). The treated eye was irradiated, while the other untreated eye served as control.

Wang Q., Dou S., Zhang B., Jiang H., Qi X., Duan H., Wang X., Dong C., Zhang B.N., Xie L., Cao Y., Zhou Q, & Shi W. (2022). Heterogeneity of human corneal endothelium implicates lncRNA NEAT1 in Fuchs endothelial corneal dystrophy. Molecular Therapy. Nucleic Acids, 27, 880-893.

+ Open protocol

+ Expand

Antibody Photoconjugation with Gx-d2 Sensors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gx-d2-SB and Gx-d2-LB were photoconjugated
to infliximab (obtained via the Máxima Medisch Centrum pharmacy
in Veldhoven, The Netherlands) and anti-IL-6 (10395-mhK23 and 10395-R508,
ordered from SinoBiological). Photoconjugation was performed with
1 μM antibody and 2 μM Gx-d2-SB or Gx-d2-LB in PBS (pH
7.4) for 15 min, by using a Thorlabs M365LP1 (λ = 365 nm) UV-lamp
coupled with a Thorlabs LEDD1B T-Cube LED Driver (continuous wave,
current limit 1.2A, 80% intensity), as shown in Figure S5. After conjugation, the crosslinked sensor components
were not further purified and stored at 4 °C until use.

van Aalen E.A., Rosier B.J., Jansen T., Wouters S.F., Vermathen R.T., van der Veer H.J., Yeste Lozano J., Mughal S., Fernández-Costa J.M., Ramón-Azcón J., den Toonder J.M, & Merkx M. (2023). Integrated Bioluminescent Immunoassays for High-Throughput Sampling and Continuous Monitoring of Cytokines. Analytical Chemistry, 95(23), 8922-8931.

+ Open protocol

+ Expand

Photolysis of Caged-Serotonin using UV LED

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used a high-power UV LED (M365LP1, 365 nm wavelength, Thorlabs) to elicit photolysis of caged-serotonin. The light was presented at 0.2 mW with a 20 ms pulse. The external saline containing caged-serotonin was recirculated at the beginning of experiments, and we replaced it every experiment. To prevent undesirable photolysis, we shielded the preparation from ambient light.

Suzuki Y., Schenk J.E., Tan H, & Gaudry Q. (2020). A population of interneurons signals changes in the basal concentration of serotonin and mediates gain control in the Drosophila antennal lobe. Current biology : CB, 30(6), 1110-1118.e4.

+ Open protocol

+ Expand

Photolysis of CNI-Gly and MNI-Glu

Check if the same lab product or an alternative is used in the 5 most similar protocols

UV-Vis absorption spectra were recorded using a Cary 50 spectrophotometer (Agilent). Photolysis used a 365 nm LED (M365LP1, Thorlabs, Sparta, NJ, USA) in HEPES buffer (40 mM, 100 mM KCl, pH 7.4) in a quartz cuvette (1 cm pathlength). Solutions of CNI-Gly and MNI-Glu having matching absorptions at 365 nm (concentrations ca. 0.05 mM) were irradiated over a period of 6 min. Changes in the absorption spectra of CNI-Gly and MNI-Glu were taken every 60 s.

Bossi S., Dhanasobhon D., Ellis-Davies G.C., Frontera J., de Brito Van Velze M., Lourenço J., Murillo A., Luján R., Casado M., Perez-Otaño I., Bacci A., Popa D., Paoletti P, & Rebola N. (2022). GluN3A excitatory glycine receptors control adult cortical and amygdalar circuits. Neuron, 110(15), 2438-2454.e8.

+ Open protocol

+ Expand

Steady-State Optical Characterization of Thin Films

Check if the same lab product or an alternative is used in the 5 most similar protocols

Steady-state absorption spectra of the thin films were measured using a Varian Cary 5000 spectrophotometer (slit width 0.5 nm). CD spectra were recorded on the same instrument using a home-built add-on employing polariser—achromatic quarter-wave plate combinations (Thorlabs WP25M-UB and AQWP05M-340 for the wavelength range 260−410 nm, Thorlabs LPVISE100-A and AQWP05M-580 for the spectral region 400−700 nm). The CD spectrum was then obtained by taking the difference of two measurements, with the fast axis of the quarter-wave plate adjusted to either +45° or −45° with respect to the axis of the polariser. Steady-state PL and CPL spectra of the complete thin film samples were obtained as follows: The thin film was illuminated by light from a filtered (Schott UG 1, 3 mm) continuous-wave UV LED (Thorlabs M365LP1, 365 nm, FWHM 10 nm). Fluorescence emitted at right angle passed through a zero-order achromatic broadband quarter-wave plate and a broadband polariser (same combination as for the steady-state CD experiments) and was then focused into a fibre-optic cable connected to a spectrograph with a back-illuminated thermoelectrically cooled CCD detector (Avantes AvaSpec-Hero). CPL spectra were obtained by subtracting the emission spectra of two consecutive measurements with the polariser axis set at either 0° or 90° and the fast axis of the quarter-wave plate fixed at 45°.

Morgenroth M., Scholz M., Cho M.J., Choi D.H., Oum K, & Lenzer T. (2022). Mapping the broadband circular dichroism of copolymer films with supramolecular chirality in time and space. Nature Communications, 13, 210.

+ Open protocol

+ Expand

Cortisol and proBNP Antibody Photoconjugation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal mouse IgG2a cortisol antibody
ab1949 (XM210) was obtained from Abcam, and monoclonal mouse IgG2a
proBNP antibody NB120–14712 (13G12cc) was purchased from Novus
Biologicals. The LUCOS sensor components (1 μM) and antibody
(1 μM) were mixed in PBS (pH 7.4) in a final volume of 20 μL
and preincubated for 30 min at room temperature. Photoconjugation
reactions were subsequently performed using a UV lamp (Thorlabs M365LP1
with a Thorlabs LEDD1B T-Cube LED Driver) for 15 min.^{6 (link)} After conjugation, the LUCOS sensors were not further purified
and stored at 4 °C until use.

van Aalen E.A., Lurvink J.J., Vermeulen L., van Gerven B., Ni Y., Arts R, & Merkx M. (2024). Turning Antibodies into Ratiometric Bioluminescent Sensors for Competition-Based Homogeneous Immunoassays. ACS Sensors, 9(3), 1401-1409.

+ Open protocol

+ Expand

Photolytic Release of cGMP

Check if the same lab product or an alternative is used in the 5 most similar protocols

Caged cGMP was photolyzed using a 365‐nm LED (M365LP1, Thorlabs). The LED was coupled to a liquid light guide and delivered to the cuvette (FC‐15 Bio‐Logic). The fluorescence background resulting from the 365‐nm LED was removed using lock‐in detection. The waveform, timing, and triggering of the photolysis light were controlled through the interface of a self‐made LabVIEW program using a DAQ card (NIUSB‐625; National Instruments). The photolytic release of cGMP was calibrated via the increase in fluorescence upon photolysis (Hamzeh et al, 2019).

Trötschel C., Hamzeh H., Alvarez L., Pascal R., Lavryk F., Bönigk W., Körschen H.G., Müller A., Poetsch A., Rennhack A., Gui L., Nicastro D., Strünker T., Seifert R, & Kaupp U.B. (2019). Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation. The EMBO Journal, 39(4), e102723.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!