Anti id3 c 20

Manufactured by Santa Cruz Biotechnology

Anti Id3 (C-20) is a laboratory antibody product manufactured by Santa Cruz Biotechnology. It is designed to detect the Id3 protein, which plays a role in cellular differentiation and proliferation processes.

Automatically generated - may contain errors

Lab products found in correlation

Anti lamin b c 20, by Santa Cruz Biotechnology (1 mentions) Anti id1 c 20, by Santa Cruz Biotechnology (1 mentions) Anti e2a n 649, by Santa Cruz Biotechnology (1 mentions) Anti β actin ac 15, by Merck Group (1 mentions) Complete mini, by Roche (1 mentions) Anti caspase 8, by Thermo Fisher Scientific (1 mentions) Anti elk 1 1 20, by Santa Cruz Biotechnology (1 mentions)

2 protocols using anti id3 c 20

Immunoblotting and Co-immunoprecipitation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in NP-40 buffer in the presence of protease inhibitor cocktail (Complete Mini, Roche Diagnostics). The following antibodies were used for immunoblotting and co-immuno-precipitation: Anti PU.1 (H-135, Santa Cruz), anti Id1 (C-20, Santa Cruz), anti Id2 (C-20, Santa Cruz), anti Id3 (C-20, Santa Cruz), anti E2A (N-649, Santa Cruz), anti Lamin B (C-20, Santa Cruz), anti Gfi1 (R&D systems) and anti β-Actin (Ac-15, Sigma-aldrich).

Fraszczak J., Helness A., Chen R., Vadnais C., Robert F., Khandanpour C, & Möröy T. (2016). Threshold Levels of Gfi1 Maintain E2A Activity for B Cell Commitment via Repression of Id1. PLoS ONE, 11(7), e0160344.

+ Open protocol

+ Expand

Immunoblot Analysis of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein lysates were sonicated and subjected to Tris-glycine sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to nitrocellulose membranes. Membranes were then blocked for 1 h with 5% nonfat milk, incubated with primary antibody overnight and secondary antibody for 1 h. The primary antibodies included: anti-Id3 (C-20; Santa Cruz Biotechnology), anti-GAPDH (EMD; Millipore, Billerica, MA), anti-active caspase-3 (Enzo Life Sciences, Inc., Farmingdale, NY), anti-caspase-8 (Thermo Scientific Rockford, IL), and anti-Elk-1 (I-20; Santa Cruz Biotechnology).

Chen Y.S., Aubee J., DiVito K.A., Zhou H., Zhang W., Chou F.P., Simbulan-Rosenthal C.M, & Rosenthal D.S. (2015). Id3 induces an Elk-1–caspase-8-dependent apoptotic pathway in squamous carcinoma cells. Cancer Medicine, 4(6), 914-924.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!