Antibodies for CD31, VEGFA, VEGFR3, podoplanin, Ki67 (ab92742, an anti‐human Ki67 antibody that does not cross‐react with the mouse Ki67), NF‐κB subunit p65 and VEGFC were purchased from Abcam (Cambridge, London, UK). HRP‐conjugated secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor 555‐conjugated secondary antibody was from Invitrogen (Carlsbad, CA, USA). Pyrrolidine dithiocarbamate (PDTC, an effective NF‐κB inhibitor) was purchased from Sigma‐Aldrich (St Louis, MO, USA). A human VEGFC ELISA Kit was purchased from R&D Systems (Minneapolis, MN, USA). TNFSF15 protein was kindly given by Professor Lu‐yuan Li (Nankai University, Tianjin, China), which was prepared as previously described.19
Human vegfc elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human VEGFC ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of human VEGFC levels in cell culture supernatants, serum, and plasma. The kit utilizes a specific antibody coated on a microplate to capture VEGFC, and a detection antibody conjugated to a reporter enzyme for quantification.
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Lab products found in correlation
Alexa fluor 555 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Pyrrolidine dithiocarbamate pdtc, by Merck Group (1 mentions)
Ab92742, by Abcam (1 mentions)
Epoch microplate reader, by Agilent Technologies (1 mentions)
5 protocols using human vegfc elisa kit
1
Immunohistochemical Analysis of Angiogenesis
2
VEGF-C Protein Quantification by ELISA
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The protein concentrations of VEGF-C were determined with human VEGF-C ELISA kit (R&D Systems) per the manufacturer’s instruction. The optical density was determined using a micro-plate reader (Sunrise, TECAN, Männedorf, Switzerland).
3
VEGF-C Quantification in Tumor Cells
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VEGF-C in the culture supernatants of tumor cells were quantified using human VEGF-C ELISA Kit (R&D Systems, USA) according to the manufacturer’s protocol.
4
Visfatin Regulates VEGF-C Secretion
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ESCC cells were plated in 6-well dishes and grown to confluence. The culture medium was then exchanged with serum-free RPMI or DMEM medium. Cells were treated for 24 h with visfatin (0, 1, 3, 10, or 30 ng/mL). CM was collected and the levels of VEGF-C expression were examined using a human VEGF-C ELISA kit (R&D Systems, MN, USA) according to the manufacturer’s protocol [79 (link), 85 (link)].
5
VEGFC Quantification in Cell Conditioned Media
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Samples of conditioned media from cells with (and without) TNFSF15 treatment were collected. Levels of VEGFC were determined using the human VEGFC ELISA Kit (R&D Systems). The absorbance was measured with an Epoch microplate reader (BioTek, Winooski, VT, USA).
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