Minispec lf90ii body composition analyzer

C57BL/6N mice were obtained from the Animal Unit of the University of Hong Kong. Mice were housed at a controlled temperature (23°C ± 1°C) with a 12-hour light/12-hour dark cycle and free access to drinking water and different types of diet: standard chow (catalog #5053, LabDiet, St. Louis, MO), rodent high-fat diet with 45 kcal% fat (D12451, Research Diets), or L-amino acid rodent diets with 60 kcal% fat with low methionine and no added choline diet (CDAHF60, Dyets). Fgf21 KO mice and their WT littermates in C57BL/6N were generated as previously described.³³ (link)^,74 (link) Genotyping was conducted as previously described.⁴⁵ (link) Adipocyte-specific MDM2 KO mice were generated by crossing MDM2^{floxed/floxed} mice with Adipoq-Cre mice (expressing Cre recombinase under the control of the adiponectin promoter; The Jackson Laboratory, Bar Harbor, ME) as previously described.³¹ (link) Mice were subjected to AAV infection or antibody treatment as indicated in each figure legend. Body composition was determined by the Bruker Minispec LF90 II Body Composition Analyzer (Bruker Biospin, Billerica, MA). Food intake was measured every week and shown as the calculated average daily calorie intake per mouse.