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Anti cd45 apc

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The Anti-CD45-APC is a fluorescently-labeled antibody that binds to the CD45 antigen on the surface of human cells. It is designed for use in flow cytometry applications to identify and quantify CD45-expressing cells.

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Lab products found in correlation

Anti cd3 fitc, by Beckman Coulter (2 mentions) Anti cd19 pe, by Beckman Coulter (2 mentions) Anti cd33 pe cy5, by Beckman Coulter (2 mentions) Anti cd15 pacific blue, by Beckman Coulter (2 mentions) Anti cd14 pe texas red, by Beckman Coulter (2 mentions) Anti cd38 pe cy7, by Beckman Coulter (2 mentions) Anti cd3 fitc, by BD (1 mentions) Anti cd3 apc, by BD (1 mentions) Anti hla dr pe, by BD (1 mentions) Anti nkp46 pe, by Beckman Coulter (1 mentions) Anti cd57 fitc, by BD (1 mentions) Anti cd56 pc7, by Beckman Coulter (1 mentions) Versalyse, by Beckman Coulter (1 mentions) Anti cd19 pc7, by Beckman Coulter (1 mentions) Cytokeratin pan type 1 2 antibody cocktail, by Thermo Fisher Scientific (1 mentions) Anti cd73 pe, by Beckman Coulter (1 mentions) Anti cd105 pe, by Beckman Coulter (1 mentions) Anti cd34 fitc, by Beckman Coulter (1 mentions) Anti cd44 fitc, by Beckman Coulter (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD45 (9 citations) CD45-APC (3 citations)

6 protocols using anti cd45 apc

1

Engraftment of AML in NSG Mice

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Animal experiments were performed in accordance with institutional guidelines approved by the UHN Animal Care Committee. 8 to 12-week-old female NOD/SCID/IL-2Rgc-null (NSG) mice were sublethally irradiated (225 cGy) 6–24 hours before transplantation. Mononuclear cells from AML patients were depleted of CD3+ cells by EasySep (Stem Cell Technologies) prior to intrafemoral transplantation. Mice were sacrificed 8 or 16 weeks after transplantation and human engraftment in the injected femur and non-injected bone marrow was evaluated by flow cytometry using the following human-specific antibodies (all used at 1:200, all from BD unless stated otherwise, catalogue number in parentheses): anti-CD45-APC (340943), anti-CD19-PE, anti-CD33-PE-Cy5, anti-CD3-FITC, anti-CD14-PE Texas Red (Beckman Coulter PNIM2707U), anti-CD15-Pacific Blue (642917), anti-CD38-PE-Cy7, and anti-CD34-APC-Cy7. The threshold for detection of human engraftment was 0.1% CD45+ cells. All flow cytometric analysis was performed on the LSRII (BD Biosciences). For limiting dilution assays, the frequency of repopulating cells was calculated using ELDA software49 (link).
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
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2

Comprehensive NK Cell Receptor Analysis

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NK cell receptors were analyzed after staining with appropriate antibody cocktail: anti-CD56-PC7 (N901), anti-NKp30-PE (Z25), anti-NKp44-PE (Z231), anti-NKp46-PE (BAB281), from Beckman Coulter; anti-CD69-FITC (FN50), anti-CD45-APC (J33), anti-CD3-PCP (UCHT1), anti-CD3-FITC (UCHT1), anti-CD3-APC (UCHT1), anti-CD57-FITC (NK-1), anti-Granzyme-B-FITC (GB11), anti-HLA-DR-PE (TU-36), anti-CD8-APC-Cy7 (SK-1), anti-CD107a-FITC (H4A3), anti-Perforin-PE (δ69) from Becton Dickinson; anti-NKG2D-APC (BAT221) from Miltenyi Biotec from R&D Systems.
Shabrish S., Karnik N., Gupta V., Bhate P, & Madkaikar M. (2020). Impaired NK cell activation during acute dengue virus infection: A contributing factor to disease severity. Heliyon, 6(7), e04320.
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3

Engraftment of AML in NSG Mice

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Animal experiments were performed in accordance with institutional guidelines approved by the UHN Animal Care Committee. 8 to 12-week-old female NOD/SCID/IL-2Rgc-null (NSG) mice were sublethally irradiated (225 cGy) 6–24 hours before transplantation. Mononuclear cells from AML patients were depleted of CD3+ cells by EasySep (Stem Cell Technologies) prior to intrafemoral transplantation. Mice were sacrificed 8 or 16 weeks after transplantation and human engraftment in the injected femur and non-injected bone marrow was evaluated by flow cytometry using the following human-specific antibodies (all used at 1:200, all from BD unless stated otherwise, catalogue number in parentheses): anti-CD45-APC (340943), anti-CD19-PE, anti-CD33-PE-Cy5, anti-CD3-FITC, anti-CD14-PE Texas Red (Beckman Coulter PNIM2707U), anti-CD15-Pacific Blue (642917), anti-CD38-PE-Cy7, and anti-CD34-APC-Cy7. The threshold for detection of human engraftment was 0.1% CD45+ cells. All flow cytometric analysis was performed on the LSRII (BD Biosciences). For limiting dilution assays, the frequency of repopulating cells was calculated using ELDA software49 (link).
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
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4

Multiparametric Flow Cytometry of Immune Cells

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Whole blood samples were diluted to 104/μl white blood cells (WBC), washed with PBS three times, and stained with fluorescent anti-CD19-PC7 (Beckman), anti CD45-APC (Beckman), anti-C1-FITC (Assaypro), anti A2M-PE (Assaypro), anti CD91-Per-CP-eF710 (Invitrogen, eBioscience), A2M-PerCP (Assaypro) and anti-GRP78 (anti-Bip, Cell Signaling Technology) antibodies. Anti-CD45 stains all the WBC while anti-CD19 marks B lymphocytes. C1 binds IgG only in its hexameric form and thus anti-C1 antibodies can detect cells to which IgG-hexamers and C1 are bound. Anti-CD91 and anti-78 kDa Glucose-Regulated Protein (GRP78) detect these A2M receptors on lymphocytes (20 (link), 21 (link)). After incubation with the antibodies, the red blood cells were lysed with VersaLyse (Beckman). All incubations were performed at room temperature, in the dark, for 10 min. Cell staining was assessed by a Flow cytometer (NAVIOS, Beckman coulter).
Naseraldeen N., Michelis R., Barhoum M., Chezar J., Tadmor T., Aviv A., Shvidel L., Litmanovich A., Shehadeh M., Stemer G., Shaoul E, & Braester A. (2021). The Role of Alpha 2 Macroglobulin in IgG-Aggregation and Chronic Activation of the Complement System in Patients With Chronic Lymphocytic Leukemia. Frontiers in Immunology, 11, 603569.
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5

Immunophenotypic Characterization of hAECs

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Immunophenotypic characterization of hAECs was assessed after isolation by flow cytometry. hAECs were fixed for 10 min at room temperature using Intraprep Kit (Beckman–Coulter Inc., Brea, CA, USA) and washed twice with PBS. Cells were incubated for 30 min at 4 °C with conjugated primary antibodies (1 μg/mL) specific for epithelial (anti-panCytokeratin (Pan-Ck)-PE, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mesenchymal (anti-CD44-FITC, anti-CD73-PE, anti-CD90-PC5, anti-CD105-PE, Beckman-Coulter Inc., Brea, CA, USA), and hematopoietic (anti-CD34-FITC, anti-CD45-APC, Beckman-Coulter Inc., Brea, CA, USA) markers. For the analysis of Pan-Ck during culture passages, we used the Cytokeratin Pan Type I/II Antibody Cocktail (MA5-13156, Thermo Scientific, Waltham, MA, USA) and Alexa Fluor 488 (A11001, Thermo Scientific, Waltham, MA, USA) as secondary antibody. After incubation, cells were washed with PBS and analyzed using the FACS Navio FC (Beckman-Coulter Inc., Brea, CA, USA) cytometer and the Kaluza FC Analysis software (Beckman-Coulter Inc., Brea, CA, USA).
Pizzuti V., Abruzzo P.M., Chatgilialoglu A., Zia S., Marrazzo P., Petrocelli G., Zannini C., Marchionni C., Poggi P., Simonazzi G., Canaider S., Alviano F., Facchin F, & Bonsi L. (2022). A Tailored Lipid Supplement Restored Membrane Fatty Acid Composition and Ameliorates In Vitro Biological Features of Human Amniotic Epithelial Cells. Journal of Clinical Medicine, 11(5), 1236.
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6

Immune Cell Profiling in Leukapheresis

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The quantity and percentage of various immune cell types in leukapheresis product and elutriation fractions were analyzed by an ABX Pentra 60 hematology analyzer (HORIBA). For other experiments, FACS analysis (Accuri C6 Plus; Beckman Coulter Life Sciences) was used. Monocytes were enumerated by CD45 + /CD14 + and propidium iodideÀnegative population (Beckman Coulter Life Sciences). Dendritic cells were gated by forward scatter and side scatter on the basis of cell size and cellular complexity, and further gated by anti-CD45 and propidium iodide. Phenotypic analyses of dendritic cells were performed using anti-CD209-APC, CD80-PE, CD83-PE, CD86-PE, CD11c-PE, HLA-DR-APC, HLA-ABC-APC and CCR7-APC (Beckman Coulter Life Sciences). To analyze cell type compositions in CUD-002 product, anti-CD45-APC, CD19-PE, CD20-PE, CD3-Percp-Cy5.5, CD56-PE, CD34-PE, lineage cocktail (CD3/CD14/CD16/CD19/CD20/CD56)-FITC, CD13-PE, CD33-PE and S100A9-PE (Beckman Coulter Life Sciences) were used.
Li Q., Yang C., Tian H., Jiang J., Li P., Zhu X., Lei T., Yin R., Ding P., Bai P, & Li Q. (2023). Development of a personalized dendritic cell vaccine and single-cell RNA sequencing-guided assessment of its cell type composition. Cytotherapy, 25(2).
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