Hplc grade trifluoroacetic acid

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

HPLC grade trifluoroacetic acid is a volatile organic acid used as a mobile phase additive in high-performance liquid chromatography (HPLC) applications. It is a colorless, fuming liquid with a pungent odor. The product is suitable for use in HPLC analysis due to its high purity and low level of impurities.

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Lab products found in correlation

6 protocols using hplc grade trifluoroacetic acid

In vitro Biopharmaceutical Characterization

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APIs: sulfamethoxazole (SMX) and paracetamol (PRC) were obtained from Fisher Scientific (UK). Furosemide (FRS), itraconazole (ITZ) and dipyridamole (DPL) were obtained from VWR (UK). Ibuprofen (IBU), carbamazepine (CBZ) and metformin (MTF) were obtained from Fagron (UK). Excipients: HPMC-Sigma was obtained from Sigma Aldrich (UK). Pharmacoat 606 and Pharmacoat 615 were obtained from Shinetsu (Japan). Chemicals: acetic acid (> 99.7%), hydrochloric acid 36.5–38%, high-performance liquid chromatography (HPLC) grade methanol, HPLC grade acetonitrile, dichloromethane, pepsin (from porcine) were obtained from Sigma-Aldrich (UK). Maleic acid, sodium chloride, sodium hydroxide, potassium phosphate monobasic, sodium dihydrogen orthophosphate dihydrate, disodium hydrogen orthophosphate dihydrate, potassium dihydrogen orthophosphate, anhydrous sodium sulfate, HPLC grade trifluoroacetic acid were obtained from Fisher Scientific (UK). Sodium taurocholate (Prodotti Chimici Alimentari S.P.A., Italy), egg lecithin–Lipoid EPCS (Lipoid GmbH, Germany), were obtained from the sources specified. Water was ultra-pure (Milli-Q) laboratory grade. Filters: Whatman® 13 mm cellulose nitrate filters 0.45 μm pore size and polytetrafluoroethylene (PTFE) 13 mm filter 0.45 μm pore size were purchased from Fisher Scientific (UK).

Zarmpi P., Flanagan T., Meehan E., Mann J, & Fotaki N. (2020). Biopharmaceutical Understanding of Excipient Variability on Drug Apparent Solubility Based on Drug Physicochemical Properties: Case Study—Hypromellose (HPMC). The AAPS Journal, 22(2), 49.

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Analytical Methods for OI338 Quantification

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Tablets for testing contained OI338 and excipient components and were prepared according to standard pharmaceutical techniques. Placebo tablets for recovery and accuracy testing were of identical excipient composition and were produced internally. Analytical reference material OI338 for standards was produced by Novo Nordisk. Analytical grade KH 2 PO 4 , (NH 4 ) 2 HPO 4, NaH 2 PO 4 , NaCl, Titrisol ampoules 1N NaOH and HCl used for pH adjustments, HPLC grade acetonitrile, methanol and 2-propanol were all purchased from Merck (Darmstadt, Germany). Analytical grade KCl was purchased from AppliChem (Darmstadt, Germany). HPLC grade H 3 PO 4 solution (49.5-50.5%) was purchased from Sigma-Aldrich (St. Louis, MO, US), HPLC grade trifluoroacetic acid was purchased from Fisher Scientific (Loughborough, UK). Biotechnology grade Brij-35 was purchased from G-Bioscience (St. Louis, MO, US). Deionized water (18 M Ω ) was prepared in the laboratory with a Millipore-Q system (Darmstadt, Germany) for all aqueous solutions. When required, media preparations were pH controlled using Meterlab PHM220 pH meter by Radiometer Analytical (Lyon, France). Two-point calibrations were conducted before media measurement by using reference solutions pH 1.679, 4.005 and 7.000 from Hach (Düsseldorf, Germany).

Fileš I, & Andersson V. (2023). Automated sample preparation of protein solid dosage forms: Novel application for the tablet processing workstation. SLAS technology, 28(4).

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Walnut Meal Protein Extraction

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Walnut meal with a protein content of 77.21 ± 2.81 %, which was treated by hydraulic pressure, was provided by Huangjinlong Edible Oil Co., ltd. (Hebei, China). Elastase, formic acid with chromatographic purity, and N-succinyl-Ala-Ala-Ala-p-nitroaniline were purchased from Sigma Chemical Co. (St. Louis, USA). Epigallocatechin-gallate (EGCG), pepsin, and trypsin were obtained from Yuanye Biotechnology Co., ltd. (Shanghai, China). Alcalase (62000 U/g) was purchased from Novozymes (China) Biotechnology Co. High-performance liquid chromatography (HPLC) grade acetonitrile was provided by Beijing Mreda Technology Co., ltd. (Beijing, China). HPLC grade trifluoroacetic acid was purchased from Thermo Fisher Scientific (Waltham, USA). All other chemicals and reagents were of analytical grade.

Xiong Y., Peng P., Chen S.J., Chang M., Wang Q., Yin S.N, & Ren D.F. (2022). Preparation, identification, and molecular docking of novel elastase inhibitory peptide from walnut (Juglans regia L.) meal. Food Chemistry: Molecular Sciences, 5, 100139.

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Analytical Grade Reagent Preparation

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Chemicals were analytical grade unless specified. Acetic acid, HPLC grade water and HPLC grade acetonitrile were purchased from J. T. Baker (Center Valley, PA). Tris buffer and HPLC grade trifluoroAcetic acid (TFA) were purchased from Thermo Fisher Scientific (Tempe, AZ). AEBSF hydrogen chloride was purchased from G-Bioscience (St. Louis, MO). Phosphate-buffered saline (PBS) was purchased from Hyclone (Logan, UT). Sodium hydroxide was purchased from VWR (Radnor, PA), and ethanol was purchased from Warner-Graham Company (Cockeysville, MD). The Amicon Ultra centrifugal filters (0.5 mL or 15 mL capacities) with molecular weight cut-offs (MWCOs) of 10 kDa or 30 kDa were purchased from Millipore Sigma (Burlington, MA).

Cai C.X., Schneck N.A., Harris D., Blackstock D., Ivleva V.B., Cheng K.C., Charlton A., Arnold F.J., Cooper J.W, & Lei Q.P. (2019). Quantification of residual AEBSF-related impurities by reversed-phase liquid chromatography. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1116, 19-23.

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HPLC Purification and Characterization of Sepia Melanin

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Acetonitrile and methanol HPLC grade (≥99.9%) were purchased from Fisher scientific (Merelbeke, Belgium). Trifluoroacetic acid HPLC grade was purchased from Fisher scientific (Loughborough, UK). C₁₈ grafted silica for flash high throughput purification was purchased from Supelco (Bellefonte, PA, USA, batch SP98226). Fontainebleau sand, hydrochloric acid 37%, hydrogen peroxide 30% and sodium sulfite were purchased from VWR Chemicals (Leuven, Belgium). Formic acid ULC/MS grade (99%) was purchased from Biosolve (Valkenswaard, The Netherlands). Sodium hypochlorite 9.6% was purchased from Notilia (Nîmes, France). Sepia officinalis eumelanin was purchased from Sigma-Aldrich (Burlington, MA, USA, batch #103H1023). Ultrapure water (0.055 μS/cm) was obtained by pre-filtration and reverse osmosis system (Labostar Pro TWF, Evoqua water technologies, Pittsburgh, PA, USA). Xanthurenic acid was purchased from Interchim (Montluçon, France, batch V0226P002).

Bonnard M., Boury B, & Parrot I. (2021). Xanthurenic Acid in the Shell Purple Patterns of Crassostrea gigas: First Evidence of an Ommochrome Metabolite in a Mollusk Shell. Molecules, 26(23), 7263.

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Synthesis and Characterization of Umbelliferones

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Umbelliferone (99% purity) was purchased from Acros Organics (NJ, USA), trifluoroacetic acid (HPLC grade) was purchased from Fisher Scientific (Loughborough, UK), and hexamethylenetetramine (≥99% purity) was purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Other reagents and solvents were purchased from commercial suppliers (Sigma-Aldrich, St. Louis, MO, USA; Acros Organics, Geel, Belguim; Fisher Scientific, Loughborough, UK; J.T. Baker, Deventer, Netherlands) and used without additional purification. All the organic solvents were of HPLC grade.
Ultra-pure water (18.2 MΩ cm) produced on an OMNI-Analytic water purification system (Xiamen RSJ Scientific Instruments Co. Xiamen, China) was used for preparation of all aqueous solutions, as well as for spectral and HPLC analyses. Phosphate buffered saline (PBS, pH 7.4 ± 0,1, Biotechnology grade, VWR Life Science AMRESCO, Solon, OH, USA) was prepared by dissolving the tableted PBS in ultra-pure water. Stock solutions of all the umbelliferones were prepared in distilled ethanol and stored in the dark.

Skarga V.V., Negrebetsky V.V., Baukov Y.I, & Malakhov M.V. (2021). Twice as Nice: The Duff Formylation of Umbelliferone Revised. Molecules, 26(24), 7482.

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