Agraquant aflatoxin b1 elisa test kit

Determination of Aflatoxin B1 content in maize samples collected at different locations in the Republic of Serbia (Table 3 and Table 4) in 2019 and 2020 was performed using AgraQuant^® Aflatoxin B1 ELISA Test Kit (Romer Labs GmbH, Tulln an der Donau, Austria). Each ground maize sample (20 g) was extracted with 100 mL of 70% methanol and vigorously shook using Benchtop Shaking Incubator 222DS (Labnet International Inc., Edison, NJ, USA) at 200 rpm for 5 min. The sample was allowed to settle, and the top layer of the extract was filtered through a Whatman #1 filter paper (Whatman, UK). Afterwards, 100 µL of the collected filtrate was diluted using the assay buffer. To perform the analysis, 100 µL of each sample or standard was mixed with 200 µL of the conjugate in individual dilution wells, and then 100 µL from each dilution well was transferred to a respective antibody-coated microwell. After 15 min of incubation at room temperature, the plate was washed with distilled water and 100 µL of substrate solution was added to each well, allowed to incubate for 5 min, and then stop solution (100 µL for each well) was added. The absorbance of each well was read at 450 nm (reference wavelength 630 nm) within 10 min after the addition of stop solution using the Microplate Photometer HiPo MPP-96 (BioSan, Rīga, Latvia).

Romer AgraQuant® Aflatoxin B1 ELISA test kit (Romer Labs, Austria) has a declared quantitative range between 2 and 50 ppb AFL B1 with a 2 ppb limit of detection and a 2 ppb limit of quantitation. The declared reactivity is 100% for AFL B1, 8.4% for aflatoxin B2, 16% for aflatoxin G1, and <0.01% for aflatoxin G2.
This ELISA kit is ideal for measuring multiple samples in parallel, up to 42 samples, with an incubation period of 15 minutes. The kit is manufactured in a ready-to-use format to reduce handling errors. The kit contains five AFL B1 standards (0 ppb, 2 ppb, 5 ppb, 20 ppb, and 50 ppb), antibody-coated plate, conjugate, substrate, and stop solution.