After 1 week of feeding to facilitate recovery, mice were assigned to lesion or sham groups via matched-pair random assignment. Mice were anesthetized with isoflurane and fixed in a stereotaxic apparatus (1900 Stereotaxic Alignment System, David Kopf Instruments, Tujunga, CA) as previously described (Brigman et al., 2013 (link)). A 33-gauge infusion cannula (Plastics One, Roanoke, VA) attached with polyurethane tubing to a Hamilton syringe (Hamilton, Reno NV) was directed at 6 sites bilaterally targeting the hippocampus (−1.50, −1.80 and −2.25 mm AP, ±1.00, ±1.40 and ±1.75 mm ML, −2.00, −2.00 and −2.25 mm DV to Bregma). 0.2 μL N-methyl-D-aspartate (12.5 mg/mL, Sigma-Aldrich, St. Louis, MO) or saline vehicle was infused over 5 min using a pump (GenieTouch, Kent Scientific, Torrington, CT), with the cannula left in place for an additional 2.5 min to allow full diffusion. On completion of the last infusion, mice were sutured, given .05 mL Diazepam (.5 mg/mL), with an additional .025 ml as needed, to control seizures and returned to their home cages. Mice were given 1 week of recovery before being returned to food restriction. Mice began TUNL testing approximately 2 weeks after completion of surgery.
1900 stereotaxic alignment system
Manufactured by Kopf Instruments
The 1900 Stereotaxic Alignment System is a precision instrument designed for positioning and aligning research subjects or samples during scientific experiments. It provides a stable and adjustable platform to hold the subject in a fixed orientation, enabling accurate and repeatable measurements or procedures.
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Lab products found in correlation
2 protocols using 1900 stereotaxic alignment system
1
NMDA-Induced Hippocampal Lesions in Mice
2
Nanoparticle Delivery to Mouse Cortex
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At approximately 6 months of age, mice were anesthetized with isoflurane and fixed in a stereotaxic apparatus (1900 Stereotaxic Alignment System, David Kopf Instruments, Tujunga, CA) as previously described [20 (link)]. A 33-gauge infusion cannula (Plastics One, Roanoke, VA) attached with polyurethane tubing to a Hamilton syringe (Hamilton, Reno NV) was directed at secondary motor cortex (M2. AP: 1.345, ML: +/- 0.6, DV: − 1.0). Y2(SiO4)O-BSA-fluorescein nanoparticles (0.5 μl) or saline vehicle was infused over 3 min using a pump (GenieTouch, Kent Scientific, Torrington, CT), with the cannula left in place for an additional 1 minute to allow diffusion. Twelve mice were assigned to four groups (n = 3 per group): 3 mg/ml (one hemisphere) + saline (other), 1 mg/ml (one hemisphere) + saline (other) and sacrificed at 24 hours. For mice sacrificed at 72 hours or 9 days, they were provided bilateral injections of nanoparticles (3 mg/ml in one hemisphere + 1 mg/ml other hemisphere).
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