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Anti atp6v1c1 h 300

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Anti-Atp6v1c1 (H-300) is an antibody product offered by Santa Cruz Biotechnology. It is a rabbit polyclonal antibody that targets the Atp6v1c1 protein. The core function of this antibody is to detect and bind to the Atp6v1c1 protein in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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3 protocols using anti atp6v1c1 h 300

1

Comprehensive Gene Expression Analysis

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These assays were performed as described previously [43 (link), 59 (link)]. The gene-specific primers are as follows: for mouse Atp6v1c1, we used the same primers as described in [18 (link), 43 (link)]; for β-actin (expected product of 517 bp) sense 5’-CATTGAACATGGCATTGTTACC-3’ and antisense 5’-CAGCTCATAGCTCTTCTCCAGG-3’; for human ATP6V1C1 (common primers for C1-a and C1-b; expected product C1a: 219bp; C1b: 165bp) sense: 5’-ATGACTGAGTTCTGGCTTATATC-3’ and antisense: 5’-AGCTACTTTCTTAACCACTCC-3’; for human ATP6V1C2 (common primers for C2-a and C2-b; expected product C2a: 487bp; C2b: 349bp) sense: 5’-CGAATCTCTCTCAGACATGG-3’ and antisense: 5’-CTGGAAGTTCACTGGTAGTCC-3’. Anti-Atp6v1c1 (H-300) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA.). Antibodies to mTOR, phospho-T398 p70 S6 kinase, p70 S6 kinase, phospho-S473 Akt, Akt, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) and p44/42 MAPK (Erk1/2) from Cell Signaling Technology (Danvers, MA). Anti-tubulin (E7) and anti-LAMP-1 (1D4B) were from Developmental Studies Hybridoma Bank (DSHB). All assays were repeated three times.
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2

Western Blot Antibody Validation

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These were performed as described in our previous study [12] (link), [15] (link). Anti-Atp6v1c1 (H-300) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-tubulin (E7) was from the Developmental Studies Hybridoma Bank (DSHB, Iowa City, IA), and anti-β-actin monoclonal antibody (8H10D10) was purchased from Cell Signaling Technology (Danvers, MA). All assays were repeated 3 times.
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3

Hematoxylin & Eosin Staining and IHC

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Hematoxylin & eosin (H&E) staining and calculation were done as described previously [29 (link), 30 (link)]. Anti-ATP6v1c1 (H-300) (Santa Cruz, CA), rat monoclonal anti-F4/80 (eBioscience, San Diego, CA) and rabbit anti-mouse CD11c (Santa Cruz, Dallas, Texas, USA) were used for Immunohistochemical analysis (IHC) separately. The IHC analyses in the present study were repeated three times separately.
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