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2 protocols using hrp conjugated mouse monoclonal antibody against β actin

1

Investigating TRPV1 and HGF Signaling

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HGF was from R & D (Minneapolis, MN). 5’-Iodoresiniferatoxin (I-RTX), doxorubicin, crizotinib and SB366791 were from Tocris (R & D, Minneapolis, MN); rabbit monoclonal antibodies to, ERK1/2, CREB, pERK1/2, pCREB, p-Met, CAMKIIαand mouse monoclonal antibodies to c-Met, HRP-conjugated horse anti-mouse IgG and goat anti-rabbit IgG antibody were from Cell Signaling Technology (Danvers, MA); bafilomycin A1, rabbit polyclonal antibodies to TRPV1 and HGF, the goat polyclonal antibody against CGRP, and HRP-conjugated mouse monoclonal antibody against β-actin were from Abcam (Cambridge, UK); Alexa fluor 488-conjugated donkey anti-rabbit IgG and Alexa fluor 680-conjugated donkey anti-goat IgG and rhodamine red-X (RRX)-conjugated donkey anti-goat IgG antibody were from Jackson Laboratory (Bar Harbor, ME); Rhodamine phalloidin was from Life Technologies (Carlsbad, CA); and HRP-conjugated goat anti-chicken antibody was from Santa Cruz Biotechnology (Dallas, TX). Anti-a3V-ATPase antibody was a kind gift from Dr. Sun-Wada, Doshisha Women’s College, Japan.
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2

Elucidating Sensory Neuron Signaling

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The sources for the reagents were as follows. APETx2 and SB366791 (Tocris); zoledronic acid monohydrate (Sigma); rabbit monoclonal antibodies to, ERK1/2, CREB, pERK1/2, pCREB, HRP-conjugated horse anti-mouse IgG and goat anti-rabbit IgG antibody (Cell Signaling); bafilomycin A1, rabbit polyclonal antibodies to ASIC3, TRPV1, the goat polyclonal antibody against CGRP, and HRP-conjugated mouse monoclonal antibody against β-actin (Abcam); Alexa fluor 488-conjugated donkey anti-rabbit IgG and donkey anti-chicken IgY, and rhodamine red-X (RRX)-conjugated donkey anti-goat IgG antibody (Jackson); rhodamine phalloidin (Life Technologies); and rabbit polyclonal antibodies to RANKL, IL-6, mouse monoclonal antibody to MIP-1α, and HRP-conjugated goat anti-chicken antibody (Santa Cruz).
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