R1.2 1 3 300 or 400 mesh au holey carbon grids

Manufactured by Quantifoil

The R1.2/1.3 300 or 400 mesh Au holey carbon grids are a type of lab equipment used in electron microscopy. They provide a support structure for specimens during imaging and analysis. The grids feature a regular pattern of holes in a carbon film, which is coated with a thin layer of gold. This design allows for the efficient transfer of electrons through the specimen, enabling high-quality imaging and data collection.

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Lab products found in correlation

Titan krios, by Thermo Fisher Scientific (2 mentions) Vitrobot mark 4, by Thermo Fisher Scientific (1 mentions) K3 summit direct electron detector, by Ametek (1 mentions)

Spelling variants of this product

Quantifoil Au 300 mesh (6 citations) Au grid (4 citations) Quantifoil 2/2 grids (4 citations)

2 protocols using r1.2 1 3 300 or 400 mesh au holey carbon grids

Cryo-EM Structure Determination of V-ATPase

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Freshly purified 2.5–3.5 mg/ml V-ATPase in buffer C was applied to Quantifoil R1.2/1.3 300 or 400 mesh Au holey carbon grids (Quantifoil). The grids were then blotted and plunged into liquid ethane for flash freezing using a Vitrobot Mark IV (FEI). The grids were imaged in a 300 keV Titan Krios (FEI) with a Gatan K3 Summit direct electron detector (Gatan) in super-resolution mode using the data-collection software Serial EM.
Dark-subtracted images collected at super-resolution mode were first normalized by gain reference and binned twofold, which resulted in a pixel size of 0.833 Å with a counted rate of 23 electrons per physical pixel per second^{48 (link)}. Images were recorded for 1.8-s exposures in 60 subframes with a total dose of 60 electrons per Å² and a defocus range of −1.0 to −2.0 μm.

Wang R., Long T., Hassan A., Wang J., Sun Y., Xie X.S, & Li X. (2020). Cryo-EM structures of intact V-ATPase from bovine brain. Nature Communications, 11, 3921.

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Cryo-EM Imaging of ACAT1 Enzyme

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The nevanimibe-bound ACAT1 sample (4mg/ml native protein, not cross-linked) was applied to Quantifoil R1.2/1.3 300 or 400 mesh Au holey carbon grids (Quantifoil). The grids were then blotted and plunged into liquid ethane for flash freezing using a Vitrobot Mark IV (FEI). The grids were imaged in a 300 keV Titan Krios (FEI) with a Gatan K3 Summit direct electron detector (Gatan). Data were collected at 0.833 Å/pixel with a dose rate of 23 electrons per physical pixel per second. Images were recorded for 1.8 second exposures in 60 subframes with a total dose of 60 electrons per Å².

Long T., Sun Y., Hassan A., Qi X, & Li X. (2020). Structure of Nevanimibe-bound Tetrameric Human Sterol O-acyltransferase 1. Nature, 581(7808), 339-343.

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