Bcs coagulation analyzer

Patients II-1 and II-2 were challenged with an intravenous infusion of plasma-derived VWF containing FVIII (Wilate, Octapharma) at 50 IU/kg. VWF antigen, VWF ristocetin cofactor activity, and FVIII activity (i.e., von Willebrand panel) were clinically evaluated at the indicated time points on a Siemens BCS coagulation analyzer by the Clinical Coagulation Laboratory at Boston Children’s Hospital. A similar clearance study of plasma-derived VWF for patient I-1 has been previously reported.^{25 (link)}

Blood samples were obtained from the arterial line. Samples were obtained at baseline (prior to vessel cannulation for ECC) and hourly following initiation of ECC blood flow. Samples obtained were used to determine arterial blood gas values, activated clotting time, white blood cell count, platelet count, platelet aggregation, fibrinogen levels, and thrombin clotting time. Blood gases were analyzed by an ABL 725 blood-gas analyzer and OSM3 Hemoximeter (Radiometer Copenhagen, Copenhagen, DK) with pH, PaCO₂, PaO₂, and hemoglobin recorded. Activated clotting time was measured by a Hemochron Blood Coagulation System Model 801 (International Technidyne, Edison, NJ). White blood cell and platelet counts were analyzed by a Coulter Counter Z1 (Coulter Electronics, Hialeah, FL). Platelet aggregation was measured by a Chrono-Log Optimal Aggregometer Model 490 (Chrono-Log, Haverton, PA). Plasma fibrinogen concentrations and thrombin clotting times were measured using a Dade Behring BCS Coagulation Analyzer (Siemens, Deerfield, IL).