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Nistatine

Manufactured by Merck Group
Sourced in Spain

Nystatin is a polyene antifungal medication used to treat fungal infections. It is a naturally occurring compound produced by the bacterium Streptomyces noursei. Nystatin functions by binding to ergosterol, a component of fungal cell membranes, leading to the formation of pores and disruption of the cell membrane's integrity, which ultimately causes fungal cell death.

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4 protocols using nistatine

1

Antioxidant and Lipid Assays Protocol

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(-)-Epicatechin (EC), 2´-7´dichlorofluorescein diacetate (DCFH-DA), ampicillin sodium salt, nistatine, agar, yeast extract, fluorodeoxyuridine (FUdR), phosphate-buffered saline (PBS), cholesterol, Bradford reagent, guanidine hydrochloride (GuHCl), 2,4-dinitrophenylhydrazine (DNPH), malondialdehyde, hexanal, hexenal and 4-HNE were purchased from Sigma-Aldrich (Madrid, Spain). Dimethyl sulfoxide (DMSO) was obtained from Panreac (Barcelona, Spain) and trichloroAcetic acid from Fluka Analytical (Madrid, Spain). HPLC grade acetonitrile was from Carlo Erba (Rodano, Italy). Acetic acid was from Merck (Darmstadt, Germany). Fluorescein thiosemicarbazide was from Carbosynth (Berkshire. UK)
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2

Quercetin-Based Antimicrobial Assay

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Quercetin (Q), ampicillin sodium salt, nistatine, agar, yeast extract, fluorodeoxyuridine (FUdR), phosphate-buffered saline (PBS), cholesterol, and 2-mercaptoethanol, were purchased from Sigma-Aldrich (Madrid, Spain). Dimethyl sulfoxide (DMSO) was obtained from Panreac (Barcelona, Spain). SYBR® SelectMaster Mix and high-capacity cDNA reverse transcription kit were from Applied Biosystems (Carlsbad, CA, USA), and the Illustra™ RNAspin mini isolation kit was from GE Healthcare (Buckinghamshare, UK).
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3

Antioxidant Capacity Evaluation Protocol

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2′,7′-Dichlorofluorescein diacetate (DCFH-DA), 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), ampicillin sodium salt, nistatine, agar, yeast extract, fluorodeoxyuridine (FUdR), phosphate-buffered saline (PBS), and cholesterol were purchased from Sigma-Aldrich (Madrid, Spain). 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Fluka (Madrid, Spain). Folin reagent, dimethyl sulfoxide (DMSO) and iron trichloride (FeCl3·6H2O) were obtained from Panreac (Barcelona, Spain), and gallic acid was from Merck (Darmstadt, Germany).
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4

Bacterial Growth Assay Protocol

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(-)-Epicatechin (EC), quercetin (Quer), ampicillin sodium salt, nistatine, agar, yeast extract, cholesterol, 5-fluoro-2′-deoxyuridine (FUdR), and bovine serum albumin were purchased from Sigma-Aldrich (Madrid, Spain). Sodium chloride, calcium chloride, 10% w/v sodium hypochlorite solution, hydrogen chloride, sodium hydroxide and dimethyl sulfoxide (DMSO) were obtained from Panreac (Barcelona, Spain). Potassium dihydrogen phosphate, potassium monohydrogen phosphate, sodium monohydrogen phosphate, acetic acid and magnesium sulfate were from Merck (Darmstadt, Germany). Petri plates Ø 35 and 60 mm were from Brand GMBH (Wertheim, Germany) and Petri plates Ø 90 mm from Francisco Soria Melguizo (Valdemoro, Madrid). MRS Broth was from Fisher Scientific (Madrid, Spain) and tryptone medium from Fluka Analytical (Madrid, Spain).
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