Poly l histidine

Manufactured by Merck Group

Sourced in United Kingdom

Poly L-histidine is a synthetic homopolymer of the amino acid L-histidine. It is commonly used as a laboratory reagent in various applications, such as protein purification and characterization. Poly L-histidine has a specific molecular structure and physicochemical properties that make it useful in these contexts.

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Close spelling variants of this product

L-histidine (185 citations) Histidine (163 citations)

3 protocols using poly l histidine

Recombinant HRP2 and NRG1 Treatment

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Plasmodium falciparum recombinant HRP2 (BioRad cat #PIP001, Hercules, CA) was prepared by expression in E.coli. The vehicle used for this protein is sodium carbonate buffer (ph = 9.6) with <0.1% sodium azide, while NRG1/carrier free (no bovine serum albumin added in its preparation) (Biotechne cat#377-HB-050/CF, Minneapolis, MN) is commercially available as lyophilized powder and was reconstituted in sterile PBS. As negative controls for HRP2, cells/organoids in basal condition were treated with the same dilution (∼1:250) of HRP2 vehicle or 4.2 μg/ml L-histidine, and Poly L-histidine (Sigma, Burlington, MA). In addition, cells or organoids were treated with 4.2 μg/ml HRP2 (Storm et al., 2019 (link)) for 8 hours, then 100 ng/ml NRG1 (concentration within physiological range) was added (Liu et al., 2018 (link)) for 18 hours.

Harbuzariu A., Nti A., Harp K.O., Cespedes J.C., Driss A, & Stiles J.K. (2022). Neuregulin-1/ErbB4 signaling modulates Plasmodium falciparum HRP2-induced damage to brain cortical organoids. iScience, 25(6), 104407.

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Fabrication and Functionalization of Biomimetic Surfaces

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Graphite powder, trypsin from bovine pancreas, poly-l-lysine hydrobromide (wt 4000–15 000), poly-l-arginine hydrochloride (wt 15 000–70 000), poly-l-histidine (wt 5000–25 000), protamine sulfate from salmon, heparin, dextran sulfate sodium salt from Leuconostoc spp. (wt > 500 000), N-acetyl-de-O-sulfated heparin sodium, hyaluronic acid sodium salt from Streptococcus equi, dextran sulfate, chondroitin sulfate A, chitosan (medium molecular weight), trypsin from bovine pancreas (TPCK-treated, essentially salt-free, lyophilized powder, ≧10 000 BAEE units mg⁻¹ protein), and the other chemicals were obtained from Sigma-Aldrich and used without further purification. Spectra/Por 6 dialysis tubing, 1000 MWCO was obtained from Spectrum Europe BV. Water was purified using a Millipore filtration system.

Lin Y., Chapman R, & Stevens M.M. (2015). Integrative Self-assembly of Graphene Quantum Dot and Biopolymer into a Versatile Biosensing Toolkit. Advanced functional materials, 25(21), 3183-3192.

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Multicomponent Biomolecular Characterization

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Poly‐l‐lysine (Br⁻; mean DP by viscometry 115), poly‐l‐arginine (Cl⁻; DP 61), poly‐l‐histidine (Cl⁻; DP 106), poly‐l‐glutamate (Na⁺; DP 240), boric acid, lead nitrate, l‐ascorbic acid, dehydro‐l‐ascorbic acid, sugars, alditols, myo‐inositol, polyamines, l‐lysine and buffer components were all from Sigma–Aldrich (Dorset, UK).

Chormova D, & Fry S.C. (2015). Boron bridging of rhamnogalacturonan‐II is promoted in vitro by cationic chaperones, including polyhistidine and wall glycoproteins. The New Phytologist, 209(1), 241-251.

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