The apoptosis of RAW264.7 cells was analysed using the flow cytometry assay. The RAW264.7 cells (1 × 105 cells/mL) were collected in a 15 mL tube after treatment. Following staining with Annexin V-APC and propidium iodide (PI) reagent (KeyGen BioTech, China) for 15 min at room temperature in dark and the cell apoptosis was rapidly analysed using a FSCAN flow cytometer (BD Biosciences, USA).
Fscan flow cytometer
Manufactured by BD
Sourced in United States
The FSCAN flow cytometer is an instrument designed for the analysis and sorting of cells or particles in a fluid stream. It utilizes light scattering and fluorescence detection principles to gather data on the properties of individual cells or particles passing through a laser beam. The FSCAN provides measurements of size, granularity, and the presence of specific fluorescent markers on the cells or particles.
Automatically generated - may contain errors
7 protocols using fscan flow cytometer
1
Flow Cytometric Analysis of RAW264.7 Apoptosis
2
Apoptosis Analysis via Flow Cytometry
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Cell apoptosis was conducted using flow cytometry analysis. Briefly, cells were seeded
into 6-well plates at density of 3 × 105/well and were stained with Annexin
V/PI double staining kit (BD Biosciences, Massachusetts) strictly according to the
manufacturer’s instruction. Cell apoptosis was then analyzed using a FSCAN flow cytometer
(BD Biosciences). All experiments were performed in triplicate.
into 6-well plates at density of 3 × 105/well and were stained with Annexin
V/PI double staining kit (BD Biosciences, Massachusetts) strictly according to the
manufacturer’s instruction. Cell apoptosis was then analyzed using a FSCAN flow cytometer
(BD Biosciences). All experiments were performed in triplicate.
3
Cell Cycle and Apoptosis Analysis
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FCM analysis for cell cycle and apoptosis was conducted 72 hours posttransfection, using the nuclear stain DAPI (40,6-diamidino-2-phenylindole) or Annexin V-FITC/7-AAD Kit. Then, the apoptosis characteristics of each group were analyzed using a FSCAN flow cytometer (BD Biosciences, USA) [22 (link)].
4
Apoptosis Quantification by Flow Cytometry
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Cellular apoptosis assay was performed by flow cytometry as previously described [24 (link)]. HL-1 cells with different additional treatments were subsequently stained with Annexin V-FITC paired with 7-AAD (640922, BioLegend, San Diego, CA, USA). The percentage of cellular apoptosis was then determined using the FSCAN flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
5
Annexin V/PI Cell Apoptosis Assay
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Cell apoptosis was measured by flow cytometry analysis. In brief, cells with a density of 2 × 105/well were seeded into six-well plates and stained with Annexin V/PI double-staining kit (BD Biosciences, MA, USA). The cell apoptosis was then determined using a FSCAN flow cytometer (BD Biosciences).
6
Quantifying Apoptosis by Flow Cytometry
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After treatment, the cells (1×10 5 cells/mL) were collected in 15 mL tubes and stained with Annexin V-APC and propidium iodide (PI) reagents (KeyGen BioTech, Nanjing, China) for 15 min at room temperature in the dark. The degree of apoptosis in the three cell groups was then rapidly analyzed using an FSCAN flow cytometer (BD Biosciences).
7
Apoptosis Measurement by Flow Cytometry
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Cell apoptosis was measured by flow cytometry. Cells were stained with Annexin V/PI Double Staining Kit (BD Biosciences, MA, USA) and detected on the FSCAN flow cytometer (BD Biosciences) [56 (link)].
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