Drp1 antibody

Manufactured by BD

Sourced in United States

The Drp1 antibody is a laboratory reagent used in biochemical and cell biology research. It is an antibody that specifically binds to the Drp1 (Dynamin-related protein 1) protein, which is involved in the regulation of mitochondrial dynamics. The Drp1 antibody can be used to detect and study the expression and localization of the Drp1 protein in various experimental systems.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Cytochrome c, by Cell Signaling Technology (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Poly adp ribose polymerase parp antibody, by Cell Signaling Technology (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions)

Close spelling variants of this product

Mouse monoclonal anti-DRP1 antibody (4 citations) Anti-Drp1 antibody (9 citations)

2 protocols using drp1 antibody

Organelle Localization Immunofluorescence Protocol

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Endogenous NS5A was detected by a mouse monoclonal antibody from Millipore (MAB8694). Drp1 antibody was purchased from BD Biosciences (611112). For co-localization studies, antibodies of several organelle markers were being used. Mitochondrial heat shock protein 70 kDa (mtHSP70) was used as a marker for mitochondria and was detected by mouse monoclonal antibody JG1 (Abcam, AB2799). Calnexin was used as an endoplasmic reticulum (ER) marker and detected with rabbit polyclonal antibodies from Sigma (C4731). PI4KA protein was detected by rabbit polyclonal antibody from Cell Signaling (4902S). Rabbit antibodies against mitofusin 2 (MFN-2) (Cat No. AAS20424C) were purchased from Antibody Verify (USA).
All the secondary antibodies conjugated with fluorophores for immunofluorescence studies were purchase from Molecular Probes. These were Alexa Fluor 488 (A-11017), 568 (A-11019), 635 (A-31575) conjugated goat anti-mouse and 488 (A-11070), 568 (A-21069) conjugated goat anti-rabbit IgG (H+L) antibodies.

Siu G.K., Zhou F., Yu M.K., Zhang L., Wang T., Liang Y., Chen Y., Chan H.C, & Yu S. (2016). Hepatitis C virus NS5A protein cooperates with phosphatidylinositol 4-kinase IIIα to induce mitochondrial fragmentation. Scientific Reports, 6, 23464.

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Breast Cancer Cell Line Culturing

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All human breast cancer cell line protocols were approved by the Ethics Committee Tianjin Medical University. The human breast cancer cell lines MCF-7 and MDA-MB-231 were preserved by our laboratory and cultured in Dulbecco’s Modified Eagle’s Medium (HyClone, Beijing, People’s Republic of China), supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin (Sigma-Aldrich Co., St Louis, MO, USA), and 100 μg/mL streptomycin (Sigma-Aldrich Co.). The Drp1 antibody was purchased from BD Biosciences (San Jose, CA, USA). Cytochrome c, poly (ADP-ribose) polymerase (PARP) antibody, and β-actin antibody were obtained from Cell Signaling Technology (MA, USA).

Jia Y., Zhou L., Tian C., Shi Y., Wang C, & Tong Z. (2015). Dynamin-related protein 1 is involved in micheliolide-induced breast cancer cell death. OncoTargets and therapy, 8, 3371-3381.

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