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Human tnf alpha elisa kit

Manufactured by RayBiotech
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The Human TNF-alpha ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human tumor necrosis factor-alpha (TNF-alpha) levels in biological samples such as cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Catalase from bovine liver, by Merck Group (1 mentions) Sodium arsenite solution, by Merck Group (1 mentions) Prostaglandin e2 eia kit, by Cayman Chemical (1 mentions) Nfat4, by Santa Cruz Biotechnology (1 mentions) Superoxide dismutase sod from bovine liver, by Merck Group (1 mentions) Lamin a c, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

4 protocols using human tnf alpha elisa kit

1

Diverse Reagents for Cell Signaling

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Sodium arsenite solution, catalase from bovine liver, and superoxide dismutase (SOD) from bovine liver were from Sigma-Aldrich (St. Louis, MO). GAPDH, p65, NFAT4, and lamin A/C antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA) with all other antibodies purchased from Cell Signaling Technology Inc. (Beverly, MA). Prostaglandin E2 EIA Kit was from Cayman Chemical Company (Ann Arbor, MI). The human TNF-alpha ELISA Kit was from RayBiotech, Inc. (Norcross, GA).
Wang L., Hitron J.A., Wise J.T., Son Y.O., Roy R.V., Kim D., Dai J., Pratheeshkumar P., Zhang Z., Xu M., Luo J, & Shi X. (2015). Ethanol enhances arsenic-induced cyclooxygenase-2 expression via both NFAT and NF-κB signalings in colorectal cancer cells. Toxicology and applied pharmacology, 288(2), 232-239.
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2

Quantifying TNFα and PGE2 Secretion

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TNF-alpha (TNFα) secreted into culture medium was measured using RayBio Human TNF-alpha ELISA kit following the manufacture’s protocol. Prostaglandin E2 (PGE2) secreted into culture medium was measured using Prostaglandin E2 EIA Kit following the manufacture’s protocol. Concentrations were estimated using a generated standard curve.
Wang L., Hitron J.A., Wise J.T., Son Y.O., Roy R.V., Kim D., Dai J., Pratheeshkumar P., Zhang Z., Xu M., Luo J, & Shi X. (2015). Ethanol enhances arsenic-induced cyclooxygenase-2 expression via both NFAT and NF-κB signalings in colorectal cancer cells. Toxicology and applied pharmacology, 288(2), 232-239.
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3

Plasma Cytokine and CRP Quantification

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Plasma TNF-α, IL-6 and hsCRP were determined using Human TNF-alpha ELISA Kit (Raybiotech, USA), Human Interleukin 6 ELISA Kit (AssayPro, USA) and Human hs-CRP ELISA Kit (Cusabio, USA), respectively.
Ghazali W.S., Romli A.C, & Mohamed M. (2017). Effects of honey supplementation on inflammatory markers among chronic smokers: a randomized controlled trial. BMC Complementary and Alternative Medicine, 17, 175.
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4

Measuring TNF-α Levels by ELISA

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The medium of the different conditions was collected at the end of the assay and centrifuged at 400g for 10 min. Supernatants were collected and stored at −80°C until ELISA analysis. TNF-α was measured with a human TNF-alpha ELISA Kit (RayBio). Immunoassays were performed according to manufacturer's instructions. Absorbance of ELISA plates was read on a microplate reader (Sunrise™ Tecan).
Blázquez-Prunera A., Almeida C.R, & Barbosa M.A. (2017). Human Bone Marrow Mesenchymal Stem/Stromal Cells Preserve Their Immunomodulatory and Chemotactic Properties When Expanded in a Human Plasma Derived Xeno-Free Medium. Stem Cells International, 2017, 2185351.
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