The CCK-8 assays were performed according to the manufacturer’s instructions (DOJINDO Laboratories, Kumamoto, Japan). Briefly, the cells were seeded in 96-well plates at a density of 1 × 104 cells/well, and incubated with different doses (0, 0.5, 1, 2, 4, 8 mM) of VC (Alfa Aesar, Ward Hill, MA,United States) for 3, 6, and 9 h. Then, 100 μl CCK-8 solution was added to each well. After that the cells were incubated for an additional 1–2 h. Finally, the light absorbance values were recorded at 450 nm using a Multiskan FC apparatus (Thermo Fisher Scientific, Waltham, MA, United States) and cell viability was calculated.
Multiskan fc apparatus
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Multiskan FC apparatus is a compact and versatile microplate reader designed for absorbance-based assays. It offers a wide wavelength range, flexible measurement modes, and user-friendly software to support a variety of applications in life science research and clinical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Cck 8, by Dojindo Laboratories (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Anti igg2b, by Southern Biotech (1 mentions)
Anti igg3, by Southern Biotech (1 mentions)
Streptavidin hrp, by Thermo Fisher Scientific (1 mentions)
4 protocols using multiskan fc apparatus
1
CCK-8 Assay for Cell Viability
2
Evaluating CRNDE's Impact on HCT-116 Cells
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The effect of CRNDE on the proliferation and cisplatin sensitivity of HCT-116 cells was investigated using the CCK-8 assay. Harvested HCT-116 cells were seeded into 96-well plates at a density of 5×103 cells/well and cultured overnight at 37°C with 5% CO2. After treatment, the cells were incubated with 10 ml of CCK-8 solution (Bioswamp; Wuhan Bienle Biotechnology Co., Ltd.) for 4 h at 37°C with 5% CO2. The optical density of wells was measured at 450 nm using a Multiskan FC apparatus (Thermo Fisher Scientific, Inc.).
3
MTT Cytotoxicity Assay Protocol
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Ncm460 and HCT-116 cells were seeded into a 96-well plate (5 × 103 cells/well) and maintained at 37°C with 5% CO2 until the cells adhered. After treatment, 20 μl of MTT at a concentration of 5 mg/ml was added to each well for 4 h, and 150 μl of dimethyl sulfoxide (Sigma, MO, USA) was then added to each well. The plate was shaken for 10 min, and the absorbance was evaluated using a Multiskan FC apparatus (Thermo, Waltham, MA, USA) at 490 nm.
4
ELISA for Mouse Immunoglobulin Isotypes
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Maxisorp plates were coated using one of the following Abs: anti-IgG1, anti-IgG2a, anti-IgG2b, anti-IgG3, anti-IgM (Southern Biotech). After incubation with mouse sera, revelation was performed using biotin-coupled anti-mouse immunoglobulin (Ig) (H + L) (Jackson IR), streptavidin HRP (ThermoFisher), TMB, and H2SO4. Optical densities were assessed using a ThermoScientific Multiskan FC apparatus.
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