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Sequence detections software version 1

Manufactured by Thermo Fisher Scientific
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Sequence Detections Software version 1.3 is a software application designed for analyzing and interpreting genetic sequence data. The core function of this software is to enable researchers and scientists to accurately detect and identify specific DNA or RNA sequences within complex samples.

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Lab products found in correlation

Dnase treatment and removal reagent, by Thermo Fisher Scientific (2 mentions) Taqman gene expression assay for influenza matrix, by Thermo Fisher Scientific (2 mentions) Abi 7500 real time pcr system platform, by Thermo Fisher Scientific (2 mentions) Quantitect reverse transcription kit, by Qiagen (2 mentions)

2 protocols using sequence detections software version 1

1

Quantitative RT-PCR for Influenza Virus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitative RT-PCR was performed to assess viral mRNA transcripts from infected ferret tracheal and lung samples used for sequencing. Total RNAs were treated with DNase using DNA-free DNase Treatment and Removal Reagents (Ambion, Inc, Austin, TX). cDNAs from total RNAs were generated using the QuantiTect reverse transcription kit (Qiagen Inc.). A custom-designed TaqMan gene expression assay for influenza Matrix (M) sequence (MPCONS2010), with primers that have complete homology with both 1918 and CA04 M sequences, was ordered from Applied Biosystems, Inc. Taqman experiments were performed on the ABI 7500 Real-Time PCR System platform and each sample was run in quadruplicate. Ribosomal RNA (18S) was used as endogenous control to normalize quantification of each target within tissues using Applied Biosystems Sequence Detections Software version 1.3. The relative amount of viral mRNA (log 10) is presented in the final results.
Peng X., Alföldi J., Gori K., Eisfeld A.J., Tyler S.R., Tisoncik-Go J., Brawand D., Law G.L., Skunca N., Hatta M., Gasper D.J., Kelly S.M., Chang J., Thomas M.J., Johnson J., Berlin A.M., Lara M., Russell P., Swofford R., Turner-Maier J., Young S., Hourlier T., Aken B., Searle S., Sun X., Yi Y., Suresh M., Tumpey T.M., Siepel A., Wisely S.M., Dessimoz C., Kawaoka Y., Birren B.W., Lindblad-Toh K., Di Palma F., Engelhardt J.F., Palermo R.E, & Katze M.G. (2014). The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease. Nature biotechnology, 32(12), 1250-1255.
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2

Quantitative RT-PCR for Influenza Virus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitative RT-PCR was performed to assess viral mRNA transcripts from infected ferret tracheal and lung samples used for sequencing. Total RNAs were treated with DNase using DNA-free DNase Treatment and Removal Reagents (Ambion, Inc, Austin, TX). cDNAs from total RNAs were generated using the QuantiTect reverse transcription kit (Qiagen Inc.). A custom-designed TaqMan gene expression assay for influenza Matrix (M) sequence (MPCONS2010), with primers that have complete homology with both 1918 and CA04 M sequences, was ordered from Applied Biosystems, Inc. Taqman experiments were performed on the ABI 7500 Real-Time PCR System platform and each sample was run in quadruplicate. Ribosomal RNA (18S) was used as endogenous control to normalize quantification of each target within tissues using Applied Biosystems Sequence Detections Software version 1.3. The relative amount of viral mRNA (log 10) is presented in the final results.
Peng X., Alföldi J., Gori K., Eisfeld A.J., Tyler S.R., Tisoncik-Go J., Brawand D., Law G.L., Skunca N., Hatta M., Gasper D.J., Kelly S.M., Chang J., Thomas M.J., Johnson J., Berlin A.M., Lara M., Russell P., Swofford R., Turner-Maier J., Young S., Hourlier T., Aken B., Searle S., Sun X., Yi Y., Suresh M., Tumpey T.M., Siepel A., Wisely S.M., Dessimoz C., Kawaoka Y., Birren B.W., Lindblad-Toh K., Di Palma F., Engelhardt J.F., Palermo R.E, & Katze M.G. (2014). The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease. Nature biotechnology, 32(12), 1250-1255.
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